[Quantitative cytoarchitecture point differences in experimental cortical hemiatrophy found by automatic image analysis]. / Quantitative Erfassung zytoarchitektonischer Seitendifferenzen durch automatische Bildverarbeitung am Beispiel einer experimentellen kortikalen Hemiatrophie.

For more than ten years automatic analysis procedures have been applied to explore cortical cytoarchitectonics of the brain. The investigations presented were carried out by a method described as "automatic morphocorticography". Attempts to find out quantitative criteria for early unilateral cortical alterations were carried out on a pig's brain with experimental cortical hemiatrophy caused artificially by wide spread meningeal fibrosis. Lateral differences of the relative volume density (V alpha) of all cells could be analysed for each cortical layer in detail by the automatically plotted morphocorticographs (MCG) of selected cortical parts from both hemispheres. Partial increases in the V alpha-values could be found in the whole cross-section of the cerebral cortex from the experimentally damaged hemisphere and the same value increases of between 10 and 15% were found in the 3rd an 5th cortical layer in particular. In contrast the mean values of the relative numerical cellular density in the whole cortical cross-section remained without significant changes. It can be concluded that the early alterations caused by this type of damage are predominantly due to changes in the neuropil structures.

[The effect of large-faced cerebral meningeal fibroses on the development of hemispheric volume. Experimental and morphometric studies of swine brains]. / Einfluss grossflächiger Hirnhautfibrosen auf die Entwicklung des Hemisphärenvolumens. Experimentelle und morphometrische Untersuchungen an Schweinegehirnen.

Alterations that occurred to the hemispheric structure as a result of experimentally induced meningeal fibrosis are reported in this paper. The rinsing function of cerebrospinal fluid was unilaterally stopped in the subarachnoidal space of domestic pigs by means of a viscous medium which contained soluble fatty acid salts. The animals were sacrificed 35 or 95 d after the experiments. Series of frontal sections of both cerebral hemispheres were planimetrically measured to establish differences between hemispheric cross-sections. Left-side deficits between 6 and 29% were recordable from all cases. Microscopic findings included large meningeal fibroses without visible changes to the cortex and others with destruction of cortical regions. Early alterations to cortical cytoarchitecture were investigated by means of automatic image analysis. Increased cell volume densities were recorded from the third and fifth cortical layers in cortex parts with no grossly visible alterations. The authors feel that the above alterations were primarily attributable to gradual occlusion of the lesser meningeal and cortical veins.

The effects of amphetamine on synaptic plasticity in rat's medial prefrontal cortex.

Morphometric analysis of medial prefrontal cortex (layer VI) of rats treated daily with amphetamine in a dose of 2.5 mg/kg during 3 weeks was performed on the electron microscopic level. The efficacy of the amphetamine dosage was tested on behavioral observation. Synapses on dendritic shafts and spines were studied. The density of axo-dendritic synapses increase on 74%, while the density of synapses on spine's neck decreased on 53%. Most synaptic parameters measured in axo-dendritic (1) and axo-spinous (2) synapses increased significantly under the influence of 2.5 mg/kg dose of AMPH: area of presynaptic terminal increased on 35% (1) and 21% (2), length of postsynaptic density increased on 13% (1) and 12% (2), area of spine increase on 25%. But the density of synaptic vesicles near the active zone decrease (1-on 16.5%, 2-on 20%).

The effects of haloperidol on synaptic plasticity in rat's medial prefrontal cortex.

Morphometric analysis of Medial prefrontal cortex (layer VI) of rats treated daily with haloperidol in a dose of 0.1 mg/kg during 3 weeks was performed on the electron microscopic level. The efficacy of the haloperidol dosage was tested on the amphetamine psychosis model. Synapses on dendritic shafts and dendritic spines were studied. The density of synapses on dendritic shafts increased on 51%, while on spine's neck it decreased on 19%. There were significant changes of some synaptic parameters only in axo-dendritic synapses: area of presynaptic terminal decreased on 13% (p less than 0.05), length of postsynaptic density decreased on 15% (p less than 0.05), but the density of synaptic vesicles near the active zone increased on 10% (p less than 0.05).

[Ultrastructure of the prefrontal cortex in long-term amphetamine administration]. / Ul'trastruktura prefrontal'noi kory mozga pri dlitel'nom vvedenii amfetamina.

In animals three weeks of chronic amphetamine administration at 2.5 mg/kg daily doses elicited either functional or dense-type degenerative changes in nerve cells. The former were prevailing, the latter rarely occurred in the single cells. Morphometric analysis showed sharp density increase in axodendritic synapses and density decrease in axospinal ones at the spine's neck. Apart of this, the postsynaptic dense structures were expanded considerably in axodendritic and axospinal synapses. The authors believe this ultrastructural reorganization to result from the amphetamine-elicited dopaminergic hyperfunction.

[Quantitative cytoarchitectonic analysis of human cerebral cortical fields based on the data of automatic morphocorticography]. / Kolichestvennyi tsitoarkhitektonicheskii analiz nekotorykh polei kory golovnogo mozga cheloveka po dannym avtomaticheskoi morfokortikografii.

The authors have demonstrated the stability and reproducibility of the main characteristics (form, position, and relationship between the amplitudes of characteristic extreme values) of the profiles of three fields of the human cerebral cortex (10, 17, and 44) constructed according to the findings of automatic layer-by-layer scanning of a series of sections at an interval of about 15 mm along the layer and measurements of the main morphometric parameters (volumetric ratio, number and the mean area of sections). The study has shown quantitative differences of the cytoarchitectonic structures of fields 10 and 44 which are in agreement with notions about the peculiarities of their morphofunctional organization.

[Visualization of the cytoplasmic segregation structure by using computed microscopy]. / Vizualizatsiia segregatsionnoi struktury tsitoplazmy s pomoshch'iu komp'iuternoi mikroskopii.

Cytoplasm organella segregation accompanies virtually all the changes occuring in embryonic and differentiated cells. The general scheme of mapping spatial distribution of cytoplasm texture parameters, realized using computed microscope LEITZ-T.A.S., allowed the imaging of geometrical relationship between yolk granules in the Rana Temporaria fertilized egg. The development of dorsoventral polarity in amphibian embryos was shown to be related to shape and location asymmetry of cytoplasm area containing clusters of yolk granules.

[Principles of self-adjusting staining of sections of cerebral cortex for automatic structural analysis]. / Printsipy adaptivnoi okraski srezov kory golovnogo mozga dlia avtomaticheskogo strukturnogo analiza.

The development of methods of an automatic structural analysis which uses modern methods of image processing has put forward requirements to standardize the staining of the preparations studied. However, the examination of autopsy material of the human brain shows that the standard procedure of staining does not always lead to standard results. The author presents a formalized description of changes in the quality of preparation staining at different stages, which is based on the measurement of distributions of the mean gradients of the section image obtained with the help of the textural analysis system TAS produced by Leitz. The procedure of adaptive staining based on this description is proposed, which will help to compensate for differences in tinctural properties of different samples of autopsy material.

[Automated analysis of the cerebral cortex using a television image analyzer]. / Avtomatizirovannyi analiz kory golovnogo mozga s pomoshch'iu televizionnogo analizatora izobrazheniia.

In the context of the development of approaches to a quantitative automated analysis of neural tissue, the use of television image analyzers seems to be fairly promising. A complex of programmes for the television analysis system (TAS) by "Leitz Company" has been developed to implement an automated analysis of cerebral cortex sections. At the first stage of investigation, the operator sets a plan of scanning the object in the interactive regimen and with a small magnification of the microscope. The second stage involves the focusing and discrimination of images, selection of objects for analysis and measurement of the principal stereological parameters of neurons with a large magnification and at an automatic regimen. The curves obtained of changes of the stereological characteristics of the cortex in relation to the distance to the surface are in good agreement with the cytoarchitectonic pattern observed. Thus, the use of the TAS allows for the effective automated processing of neuromorphological preparations.

[Use of a "Microvideomat" television analyzer to evaluate the functional state of a population of rat duodenal enterochromaffin cells]. / Primenenie televizionnogo analizatora "Mikrovideomat" dlia otsenki funktsional'nogo sostoianiia populiatsii énterokhromaffinnykh kletok dvenadtsatiperstnoi kishki krysy.

Application of a "Microvideomat" television image analyzer for quantitative evaluation of the Masson-Gamperl reaction enabled one to disclose slight shifts in the enterochromaffin cell population function of the rat duodenum. These shifts indicate intensification of the synthetic processes and accumulation of the secretory product 3 hours after a single food stimulation.