[Mycetoma: attempt at intra-arterial management]. / Myzetom: Versuch einer intraartericellen Behandlung.

A 28 year old African student had acquired a mycetoma of his left foot after an injury in 1975. As the surgical and antimycotic therapy had no effect, he was brought to Düsseldorf for the amputation of his foot. Madurella mycetomi was isolated from the skin, pus and bone. Before the amputation was made a last attempt to treat him with the antimycotic agent miconasol nitrate, administered intraarterially through the arteria tibialis posterior, which had been shown i arteriography to perfuse the whole foot. On the 23rd day of therapy pain and fever indicated bacterial infection. The fungus could still be isolated from the bone tissue. The therapy was terminated on the 30th day. The catheter was blocked and amputation of the food became inevitable. The patient received an artificial limb and is able to move freely. In similar cases of mycotic bone infection an attempt with intraarterial infusion of an antimycotic agent should be made immediately.

[Neisseria gonorrhoeae culture: development of an easy method]. / Neisseria-gonorrhoeae-Züchtung: Entwicklung einer vereinfachten Methode

An easy and cheap method for culturing Neisseria is developed. The medium for gonococci is prepared as proposed by the producer (BBL, Oxoid, Hoechst). About 8 ml of the medium are poured in sterile air-tight stool tubes of 25 ml volume. Materials to be examined for gonococci are taken from the cervix, ureter or anus and are inoculated on the medium. A small piece (20-30 mg) of the GasPak tablet (BBL) is then deposited in the tube and closed immediately. The GasPak tablet consists of sodium bicarbonate and citric acid which, if they come in contact with humidity, produce CO2 gas. The inoculated tube is then put in an incubator at 37 degrees C for 14-24 h. This method gives a good microbiological result. With the aid of the oxidase reaction the colonies take a brown-black color. For further differentiation of the species the sugar fermentation method is necessary.

[Experimental inoculation of Leishmaniasis tropical from man to man (author's transl)]. / Experimentelle Inoculation der Leishmaniasis tropical von Mensch zu Mensch

Experimental transformation of leishmaniasis from man to man was demonstrated. The infection occurs through direct inoculation of the leishmania organism in the amastigote form: from an ulcer of a leishmania papule some materials were obtained and were inoculated intradermally in the thigh of one of the authors. Within weeks a red papule has developed in the place of inoculation from which the microorganisms were frequenctly demonstrated. The papule was cured spontaneously within 70 weeks under a formation of a flat pigmented scar. In the electronenmicroscopy the microorganisms were seen to be located intracellular. The microorganism had a double outer membrane, an oval nucleus, and a kinetoplast out of which (in the phlebotomus fly or in the culture) the future flagellum would be formed.