ABSTRACT
Diffuse large B-cell lymphoma with MYC rearrangement is defined as double/triple-hit lymphoma (DHL/THL) or single-hit lymphoma (SHL) by the inclusion of the BCL2 and BCL6 rearrangements status. DHL/THL is called as "high-grade B-cell lymphoma with MYC and BCL2 and/or BCL6 rearrangements" in the World Health Organization 2017 Classification of Tumors of Hematopoietic and Lymphoid Tissues. To find a prognostic biomarker of DHL/THL, we firstly examined 19 cases (molecular analysis series;10 cases of DHL/THL and 9 cases of SHL) with gene expression profile analysis. The gene expression profile analysis showed that the high expression of AICDA was associated with an adverse prognosis in DHL/THL, but not in SHL. Then, we evaluated immunohistochemical expression of AID, the protein product of AICDA, in 50 cases (molecular analysis series of 19 cases and additional immunohistochemistry series of 31 cases; 12 cases of DHL/THL and 19 cases of SHL) and confirmed that its expression was also associated with an adverse prognosis in DHL/THL. Therefore, AICDA and AID can be a predictor of an adverse clinical outcome in DHL/THL and immunohistochemistry of AID is useful to find DHL/THL-adverse prognosis group.
Subject(s)
Cytidine Deaminase/analysis , Lymphoma, Large B-Cell, Diffuse , Prognosis , Adult , Aged , Aged, 80 and over , Biomarkers, Tumor/analysis , Chromosome Aberrations , Female , Gene Expression Profiling , Gene Rearrangement , Humans , Immunohistochemistry , In Situ Hybridization, Fluorescence , Lymphoma, Large B-Cell, Diffuse/genetics , Lymphoma, Large B-Cell, Diffuse/pathology , Male , Middle Aged , Proto-Oncogene Proteins c-bcl-2/genetics , Proto-Oncogene Proteins c-bcl-6/genetics , Proto-Oncogene Proteins c-myc/geneticsABSTRACT
We have investigated the role assignment and radiation exposure of medical workers (including receptionists) in PET (positron emission tomography) facilities in Japan using a questionnaire. The survey period was from October 1st to November 15th 2006. The response rate for the questionnaire was 60.0% (72/120 facilities). Nurses were engaged in the intravenous administration of radioactive FDG in 66.9% of PET facilities. In 89.5% of PET facilities, radiological technologists mainly performed the PET examination. The average radiation exposure to medical workers was 0.13 mSv/month (n: 709, S.D.: 0.16) as the effective dose. It was shown that radiation exposure was significantly different depending on the occupation and content of work (p<0.01). The radiation exposure of cyclotron operators and radiological technologists was higher than that of the other occupations (p<0.01). The highest radiation dose to one worker per a PET facility was 0.60 [mSv month(-1)], which was 4.6 times higher than the average dose of 0.13 [mSv month(-1)]. We have clarified the actual conditions of radiation protection in PET facilities in Japan for the first time.
