ABSTRACT
Immunomodulating properties of ceruloplasmin were determined in vivo on healthy mice line C57BL/6 and mice with Lewis carcinoma. It was established, that ceruloplasmin at the concentration of 1-40 mg/kg stimulated both T- and B--systems of immunity and prevented immunodepression activity of tumor growth.
Subject(s)
Adjuvants, Immunologic/pharmacology , Ceruloplasmin/pharmacology , Lung Neoplasms/immunology , Neoplasms, Experimental/immunology , Animals , B-Lymphocytes/immunology , Concanavalin A/pharmacology , Lymphocyte Activation , Mice , Mice, Inbred C57BL , T-Lymphocytes/immunology , Tumor Cells, Cultured/immunologyABSTRACT
Alternative principles of regional IL-2-dependent adoptive immunocytotherapy of malignant tumours are suggested. Regional adoptive immunocytotherapy consists in the administration of the short-term IL-2 treated lymphocytes of the lymph nodes removed from the tumour which have tumour-associated suppressor activity and spleen cells after elimination of cells with the immunosuppressive activity.
Subject(s)
Immunization, Passive , Interleukin-2/administration & dosage , Lymphocytes/immunology , Rhabdomyosarcoma/therapy , Sarcoma, Experimental/therapy , Animals , Chemotherapy, Cancer, Regional Perfusion , Lymph Nodes/cytology , Male , Mice , Mice, Inbred BALB C , Neoplasm Transplantation , Spleen/cytologyABSTRACT
Two developed experimental models based on induction of suppression by alloantigens in mice and on testing of its effect on the level of proliferative immune reaction during systemic or local adoptive transfer of spleen suppressor cells to syngeneic mice were used. Formation of immunosuppressive mechanisms has been shown to depend on alloantigen (H-2, Mls) properties. Dynamics of suppression development, a degree of its specificity, role of T cells and macrophages in suppression and sensitivity to cyclophosphamide were studied. The possibility to employ the proposed models in the studies of immunosuppression peculiarities during tumour development is considered.
Subject(s)
Immunity, Cellular , T-Lymphocytes, Regulatory/immunology , Transplantation Immunology , Animals , Immunization, Passive , Isoantigens/immunology , Macrophages/immunology , Mice , Mice, Inbred Strains , Spleen/immunologyABSTRACT
Basic theoretical and experimental preconditions for the creation of the so-called "artificial tumour" are examined. It should be an operated extracorporeal analog of the spontaneously growing blastoma with autologous tumour cells as the main structural and functional element. Construction principles and immunological aspects of the "artificial tumour" are discussed. The real potentialities of its usage for the selective elimination of the tumour-stimulating humoral factors of different origin out of the organism as well as for the specific activation of autologous effector immunocytes are analyzed.
Subject(s)
Tumor Cells, Cultured , Animals , Antibody Formation , Humans , Hybrid Cells , Tumor Cells, Cultured/immunology , Tumor Cells, Cultured/pathologyABSTRACT
The ability of lymph node and spleen cells to secrete and sorb interleukin-2 (IL-2) during the growth of sarcomas induced by 20-methylcholanthrene is studied. Lymph node cells show an increased level of IL-2 production at early stages of the tumour growth whereas spleen cells produced IL-2 in minimal amounts. A decrease in the IL-2 production by lymph node cells with the tumour growth and a relative increase of IL-2 production in the spleen cell pool are observed. At all stages of the tumour growth the ability of lymphoid cells to sorb IL-2 decreased strongly in comparison with the analogous cells of normal animals activated by mitogen. Lymph node cells of animals with tumour sorb IL-2 more effectively than splenocytes.
Subject(s)
Interleukin-2/biosynthesis , Lymphocytes/metabolism , Sarcoma, Experimental/pathology , Animals , Lymph Nodes/pathology , Mice , Mice, Inbred BALB C , Neoplasm Transplantation , Sarcoma, Experimental/chemically induced , Sarcoma, Experimental/metabolism , Spleen/pathologyABSTRACT
The level of proliferative activity is studied in the cells of thymus, spleen and lymphoid nodes of mice with transplanted OH-2 tumour once- or thrice-immunized with vaccines from syngeneic tumour cells. Single immunization is shown to induce the short-term modulation of the proliferative processes in lymphoid organs and to increase somewhat the level of antitumour resistance of vaccinized mice. The triple-inoculation of vaccines evokes a more pronounced rise in the level of the proliferative activity in cells of lymphoid organs.
Subject(s)
Lymphoid Tissue/pathology , Neoplasms, Experimental/pathology , Vaccines/administration & dosage , Animals , Cell Division , Male , Mice , Mice, Inbred A , Neoplasm Transplantation , Neoplasms, Experimental/immunology , Neoplasms, Experimental/therapy , VaccinationABSTRACT
The use of two independent test-systems (intravenous adoptive transfer of immune response on tumour cells by intact syngenic recipient and mixed cultivation of immunocompetent and tumour cells in diffusion chambers) made it possible to establish that exogenous IL-2 possesses an expressive capacity both to the positive and to the negative modulation of immunological mechanisms during the tumour growth.
Subject(s)
Interleukin-2/immunology , Rhabdomyosarcoma/immunology , T-Lymphocytes, Regulatory/drug effects , Animals , Cells, Cultured , Female , Immunity, Cellular/drug effects , Immunization , Lymph Nodes/immunology , Methylcholanthrene , Mice , Mice, Inbred BALB C , Neoplasm Transplantation , Rats , Rhabdomyosarcoma/chemically induced , Spleen/immunology , T-Lymphocytes, Regulatory/immunology , Time FactorsABSTRACT
A study was made of the effect of injection of cyclophosphamide (CP) immunomodulating to BALB/c mice, according to 3 schemes of injection with the whole dose being 200 mg/kg. In all, the state of peripheral blood and spleen, the cellular content of lymph nodes and the number of DNA-synthesizing cells in them were studied, in addition to the ability of lymph node lymphocytes to proliferative reaction on T and B cells mitogens in vivo. It was determined that a 2-fold injection of CP, in contrast to 10- and 5-fold injections, resulted in splenomegaly and leukocytosis in the experimental animals. By means of quantitative tests in vivo, in was demonstrated that the dynamics of restoration of proliferative responses to mitogens depends on the CP injection scheme. These tests proved to be more informative for registration of immunomodulating effect of CP, than the study of cellular content of lymph nodes and the number of DNA-synthesizing cells in them.
Subject(s)
Adjuvants, Immunologic , Cyclophosphamide/administration & dosage , Animals , B-Lymphocytes/drug effects , B-Lymphocytes/immunology , Concanavalin A/pharmacology , DNA/biosynthesis , Dextran Sulfate , Dextrans/pharmacology , Female , Leukocyte Count , Lymph Nodes/drug effects , Lymph Nodes/immunology , Mice , Mice, Inbred BALB C , Mitogens/pharmacology , Spleen/drug effects , Spleen/immunology , T-Lymphocytes/drug effects , T-Lymphocytes/immunology , Time FactorsABSTRACT
The application of a complex of methods for fractionation of mouse spleen cells with transplanted rhabdomyosarcomas has shown that optimal conditions for adoptive intravenous transfer of the tumour-associated suppressor activity are created under joint injection of the primed macrophages and lymphoid cells of spleen (from animals with the progressively growing tumours) interacting with the macrophages to syngenic recipients.
Subject(s)
Immunization, Passive , Rhabdomyosarcoma/immunology , Spleen/immunology , T-Lymphocytes, Regulatory/immunology , Animals , Female , Macrophages/immunology , Mice , Mice, Inbred BALB C , Neoplasm TransplantationABSTRACT
A comparative morphofunctional analysis of lymphoid cells of BALB/c mice with the transplantable tumours was performed after the cyclophosphamide (CP) treatment. CP was injected by 3 schemes in a dose of 200 mg/kg of body weight. It was found that the more effective therapeutic scheme of CP treatment is accompanied by essential normalization of the ratio of lymphocyte populations in the lymph nodes against a background of a comparatively good state of proliferating cells as well as by a higher rate of restoration of proliferative responses to T and B cell mitogens in vivo.
Subject(s)
B-Lymphocytes/immunology , Cyclophosphamide/therapeutic use , Sarcoma, Experimental/drug therapy , Soft Tissue Neoplasms/drug therapy , T-Lymphocytes/immunology , Adjuvants, Immunologic , Animals , Female , Leukocyte Count , Mice , Mice, Inbred BALB C , Neoplasm Transplantation , Sarcoma, Experimental/immunology , Soft Tissue Neoplasms/immunologyABSTRACT
The suppressor activity of the splenic cells in the cyclophosphamide (CP)-treated BALB/c mice with transplanted tumours was studied. CP was injected according to three different therapeutic effective schedules. The analysis of non-specific and antigen-specific tumour-associated suppressor activity in the splenic cell adoptive transfer system demonstrated that CP immunomodulating influence depends on the injection schedule. Usage of the most therapeutically effective CP injection schedule ensured a relative safety of the nonspecific tumour-associated suppressor activity and depression of the specific one.
Subject(s)
Cyclophosphamide/therapeutic use , Sarcoma, Experimental/drug therapy , Spleen/drug effects , T-Lymphocytes, Regulatory/drug effects , Animals , Concanavalin A/pharmacology , Female , Immunity, Innate/drug effects , Immunization, Passive , Methylcholanthrene , Mice , Mice, Inbred BALB C , Neoplasm Transplantation , Sarcoma, Experimental/chemically induced , Sarcoma, Experimental/immunology , Spleen/immunology , T-Lymphocytes, Regulatory/immunology , Time Factors , Transplantation, IsogeneicABSTRACT
A relatively simple and easily reproducible method is presented for testing the activity of tumour-associated suppressor cells in the syngeneic system. It includes adoptive transfer and radioactive assay of the inhibition degree of the specific proliferative response to tumour cells of syngeneic recipients.
Subject(s)
Rhabdomyosarcoma/immunology , T-Lymphocytes, Regulatory/immunology , Animals , Cell Division/drug effects , Cell Division/radiation effects , Concanavalin A/pharmacology , Immunization, Passive , Lymph Nodes/immunology , Lymph Nodes/radiation effects , Lymphocyte Transfusion , Lymphocytes/immunology , Lymphocytes/radiation effects , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Neoplasm Transplantation , Spleen/immunology , Spleen/radiation effects , T-Lymphocytes, Regulatory/radiation effects , Transplantation, IsogeneicABSTRACT
Two experimental models have been elaborated for E. coli 0111:B4 endotoxin effects on the growth of transplanted murine sarcomas induced by Meth A. A correlation is shown between stimulatory and inhibitory effects of endotoxin on the tumour growth and functional activity of T- and B-lymphocytes registered by in vivo tests.
Subject(s)
Endotoxins/immunology , Sarcoma, Experimental/immunology , Animals , B-Lymphocytes/immunology , Escherichia coli , Female , Immunity, Cellular , Mice , Mice, Inbred BALB C , T-Lymphocytes/immunology , Time FactorsABSTRACT
The ratio of T and B lymphocytes and DNA content in population enriched by these cells were analysed in the lymph nodes of BALB/c mice with progressively growing sarcomas induced by the MSV Moloney and in untreated mice. T lymphocyte enriched fractions have a higher DNA content in comparison to fractions enriched with B lymphocytes. Sarcoma development promotes a decrease in the relative number of macronucleolar lymphocytes and simultaneous decrease of micronucleolar ones in the lymph nodes, particularly in the regional ones.
Subject(s)
B-Lymphocytes/metabolism , DNA/metabolism , Lymph Nodes/pathology , Sarcoma, Experimental/pathology , T-Lymphocytes/metabolism , Animals , Leukocyte Count , Mice , Moloney murine leukemia virus , Sarcoma, Experimental/metabolismABSTRACT
Cell surface receptors on human lymphocytes being studied, essential differences were revealed in a relative content of E-, EA- and EAC-rosette-forming cells in peripheral blood and tonsils. In tonsils, part of lymphocytes carrying receptors for sheep erythrocytes have been shown to possess C'3-receptors for sheep erythrocytes. Apparently, C'-receptor,--being B-lymphocyte surface marker,--may also appear at definite stages of T-cell differentiation.
Subject(s)
Binding Sites, Antibody , Lymphocytes/immunology , B-Lymphocytes/immunology , Chronic Disease , Complement C3/immunology , Erythrocytes/immunology , Humans , Immunity, Cellular , Immunoglobulin Fc Fragments/immunology , Receptors, Antigen, B-Cell/immunology , Rosette Formation , Surface Properties , T-Lymphocytes/immunology , Tonsillitis/immunologyABSTRACT
E-, EA-, and EAC-reaction of rosette formation and detection of surface immunoglobulins were applied to the comparative study of the peripheral blood lymphocytes populations and tonsils in man. The relative lymphocyte content with different surface characteristics differed significantly in these populations. In detection of surface immunoglobulins on EAC-rosette forming cells it was revealed that not all the C3-lymphocytes of the tonsils bore surface Ig. A possibility of appearance of the C3-receptor on the activated T-lymphocytes is discussed.
Subject(s)
Lymphocytes/immunology , Palatine Tonsil/cytology , Receptors, Antigen, B-Cell/analysis , Animals , Binding Sites , Cell Count , Complement C3 , Erythrocytes/immunology , Palatine Tonsil/immunology , Rosette Formation , Sheep/immunologyABSTRACT
The results of separation of T- and B-cells of the C3H/Sn and AKR/j micr lymph nodes with AKR-anti-theta3H and C3H-thetaAKR isosera and cellular immunosorbents containing the antigen-antibody complexes were analyzed in this work. The strain differences in the theta+-cells (T-lymphocytes) level were demonstrated in the untreated mice of the noted strains. Populations enriched by B-lymphocytes under different methods of separation differed somewhat in composition. The cellular immunosorption had many advantages in comparison with the cytotoxic-immune sera used for the T- and B-cell separation.