[Immunomodulating activity of exogenous ceruloplasmin in experimental tumor growth]. / Immunomoduliruiushchaia aktivnost' ekzogennogo tseruloplazmina pri éksperimental'nom opukholevom protsesse.

Immunomodulating properties of ceruloplasmin were determined in vivo on healthy mice line C57BL/6 and mice with Lewis carcinoma. It was established, that ceruloplasmin at the concentration of 1-40 mg/kg stimulated both T- and B--systems of immunity and prevented immunodepression activity of tumor growth.

[Evaluation of the effectiveness of regional interleukin-2 dependent adoptive immunocytotherapy of mice with transplanted rhabdomyosarcoma]. / Otsenka éffektivnosti regionarnoi interleikin-2-zavisimoi adoptivnoi immunotsitoterapii myshei s perevivnoi rabdomiosarkomoi.

Alternative principles of regional IL-2-dependent adoptive immunocytotherapy of malignant tumours are suggested. Regional adoptive immunocytotherapy consists in the administration of the short-term IL-2 treated lymphocytes of the lymph nodes removed from the tumour which have tumour-associated suppressor activity and spleen cells after elimination of cells with the immunosuppressive activity.

[Characteristics of cellular immunosuppression models of allotransplantation reactions]. / Kharakteristika modelei kletochnoi immunosupresii allotransplantatsionnykh reaktsii.

Two developed experimental models based on induction of suppression by alloantigens in mice and on testing of its effect on the level of proliferative immune reaction during systemic or local adoptive transfer of spleen suppressor cells to syngeneic mice were used. Formation of immunosuppressive mechanisms has been shown to depend on alloantigen (H-2, Mls) properties. Dynamics of suppression development, a degree of its specificity, role of T cells and macrophages in suppression and sensitivity to cyclophosphamide were studied. The possibility to employ the proposed models in the studies of immunosuppression peculiarities during tumour development is considered.

[The concept of the "artificial tumor"]. / Contseptsiia "iskusstvennoi opukholi."

Basic theoretical and experimental preconditions for the creation of the so-called "artificial tumour" are examined. It should be an operated extracorporeal analog of the spontaneously growing blastoma with autologous tumour cells as the main structural and functional element. Construction principles and immunological aspects of the "artificial tumour" are discussed. The real potentialities of its usage for the selective elimination of the tumour-stimulating humoral factors of different origin out of the organism as well as for the specific activation of autologous effector immunocytes are analyzed.

[Characteristics of production and sorption of interleukin 2 by lymphoid cells during growth of transplanted sarcoma]. / Osobennosti produktsii i sorbtsii interleikina-2 limfoidnymi kletkami v dinamike rosta perevivnykh sarkom.

The ability of lymph node and spleen cells to secrete and sorb interleukin-2 (IL-2) during the growth of sarcomas induced by 20-methylcholanthrene is studied. Lymph node cells show an increased level of IL-2 production at early stages of the tumour growth whereas spleen cells produced IL-2 in minimal amounts. A decrease in the IL-2 production by lymph node cells with the tumour growth and a relative increase of IL-2 production in the spleen cell pool are observed. At all stages of the tumour growth the ability of lymphoid cells to sorb IL-2 decreased strongly in comparison with the analogous cells of normal animals activated by mitogen. Lymph node cells of animals with tumour sorb IL-2 more effectively than splenocytes.

[Changes in the proliferative activity of cells from lymphoid organs of tumor-bearing mice after immunization with antitumoral vaccines]. / Izmeneniia proliferativnoi aktivnosti kletok limfoidnykh organov myshei s opukhol'iu pri immunizatsii protivoopukholevymi vaktsinami.

The level of proliferative activity is studied in the cells of thymus, spleen and lymphoid nodes of mice with transplanted OH-2 tumour once- or thrice-immunized with vaccines from syngeneic tumour cells. Single immunization is shown to induce the short-term modulation of the proliferative processes in lymphoid organs and to increase somewhat the level of antitumour resistance of vaccinized mice. The triple-inoculation of vaccines evokes a more pronounced rise in the level of the proliferative activity in cells of lymphoid organs.

[Effect of exogenous interleukin 2 on the functional activity of immunocompetent cells in mice with transplanted tumors]. / Vliianie ékzogennogo interleikina-2 na funktsional'nuiu aktivnost' immunokompetentnykh kletok myshei s perevivnymi opukholiami.

The use of two independent test-systems (intravenous adoptive transfer of immune response on tumour cells by intact syngenic recipient and mixed cultivation of immunocompetent and tumour cells in diffusion chambers) made it possible to establish that exogenous IL-2 possesses an expressive capacity both to the positive and to the negative modulation of immunological mechanisms during the tumour growth.

[Immunomodulating effect of cyclophosphane under different modes of administration to mice]. / Immunomoduliruiushchee vliianie tsiklofosfana pri razlichnykh skhemakh vvedeniia ego mysham.

A study was made of the effect of injection of cyclophosphamide (CP) immunomodulating to BALB/c mice, according to 3 schemes of injection with the whole dose being 200 mg/kg. In all, the state of peripheral blood and spleen, the cellular content of lymph nodes and the number of DNA-synthesizing cells in them were studied, in addition to the ability of lymph node lymphocytes to proliferative reaction on T and B cells mitogens in vivo. It was determined that a 2-fold injection of CP, in contrast to 10- and 5-fold injections, resulted in splenomegaly and leukocytosis in the experimental animals. By means of quantitative tests in vivo, in was demonstrated that the dynamics of restoration of proliferative responses to mitogens depends on the CP injection scheme. These tests proved to be more informative for registration of immunomodulating effect of CP, than the study of cellular content of lymph nodes and the number of DNA-synthesizing cells in them.

[Characteristics of cells participating in the adoptive transfer of tumor-associated suppressor activity]. / Kharakteristika kletok, uchastvuiushchikh v adoptivnom perenose opukholeassotsiirovannoi suppressornoi aktivnosti.

The application of a complex of methods for fractionation of mouse spleen cells with transplanted rhabdomyosarcomas has shown that optimal conditions for adoptive intravenous transfer of the tumour-associated suppressor activity are created under joint injection of the primed macrophages and lymphoid cells of spleen (from animals with the progressively growing tumours) interacting with the macrophages to syngenic recipients.

[Immunomodulating effect of cyclophosphamide in experimental chemotherapy of transplanted tumors]. / Immunomoduliruiushchee deistvie tsiklofosfana pri eksperimental'noi khimioterapii perevivnykh opukholei.

A comparative morphofunctional analysis of lymphoid cells of BALB/c mice with the transplantable tumours was performed after the cyclophosphamide (CP) treatment. CP was injected by 3 schemes in a dose of 200 mg/kg of body weight. It was found that the more effective therapeutic scheme of CP treatment is accompanied by essential normalization of the ratio of lymphocyte populations in the lymph nodes against a background of a comparatively good state of proliferating cells as well as by a higher rate of restoration of proliferative responses to T and B cell mitogens in vivo.

[Effect of cyclophosphane on the suppressor activity of spleen cells in mice with transplantable tumors]. / Vliianie tsiklofosfana na supressornuiu aktivnost' kletok selezenki myshei s perevivnymi opukholiami.

The suppressor activity of the splenic cells in the cyclophosphamide (CP)-treated BALB/c mice with transplanted tumours was studied. CP was injected according to three different therapeutic effective schedules. The analysis of non-specific and antigen-specific tumour-associated suppressor activity in the splenic cell adoptive transfer system demonstrated that CP immunomodulating influence depends on the injection schedule. Usage of the most therapeutically effective CP injection schedule ensured a relative safety of the nonspecific tumour-associated suppressor activity and depression of the specific one.

[Method of assessing the functional activity of tumor-associated suppressor cells]. / Metodika otsenki funktsional'noi aktivnosti opukholeassotsiiro-vannykh kletok-supressorov.

A relatively simple and easily reproducible method is presented for testing the activity of tumour-associated suppressor cells in the syngeneic system. It includes adoptive transfer and radioactive assay of the inhibition degree of the specific proliferative response to tumour cells of syngeneic recipients.

[Immunological characteristics of a modifying effect of bacterial endotoxins on the growth of experimental tumors]. / Immunologicheskaia kharakteristika modifitsiruiushchego vliianiia bakterial'nykh éndotoksinov na rost éksperimental'nykh opukholei.

Two experimental models have been elaborated for E. coli 0111:B4 endotoxin effects on the growth of transplanted murine sarcomas induced by Meth A. A correlation is shown between stimulatory and inhibitory effects of endotoxin on the tumour growth and functional activity of T- and B-lymphocytes registered by in vivo tests.

[Lymph node cell composition and DNA content in their different lymphocyte populations in mice with tumor induced by murine Moloney sarcoma virus]. / Kletochnyi sostav limfaticheskikh uzlovi soderzhanie DNK v razlichnykh populiatsiiakh ikh limfotsitov u myshei s opukhol'iu, indutsirovannoi myshinym sarkomatoznym virusom Moloni.

The ratio of T and B lymphocytes and DNA content in population enriched by these cells were analysed in the lymph nodes of BALB/c mice with progressively growing sarcomas induced by the MSV Moloney and in untreated mice. T lymphocyte enriched fractions have a higher DNA content in comparison to fractions enriched with B lymphocytes. Sarcoma development promotes a decrease in the relative number of macronucleolar lymphocytes and simultaneous decrease of micronucleolar ones in the lymph nodes, particularly in the regional ones.

[Immunological analysis of human lymphocytes bearing different surface receptors]. / Immunologicheskii analiz limfotsitov cheloveka, nesushchikh rezlichnye poverkhnostnye retseptory.

Cell surface receptors on human lymphocytes being studied, essential differences were revealed in a relative content of E-, EA- and EAC-rosette-forming cells in peripheral blood and tonsils. In tonsils, part of lymphocytes carrying receptors for sheep erythrocytes have been shown to possess C'3-receptors for sheep erythrocytes. Apparently, C'-receptor,--being B-lymphocyte surface marker,--may also appear at definite stages of T-cell differentiation.

[Comparative study of different types of rosette-forming cells in human peripheral blood and tonsils]. / Sravnitel'noe izuchenie razlichnykh vidov rozetkoobrazuiushchikh kletok perifericheskoi krovi i mindalin cheloveka.

E-, EA-, and EAC-reaction of rosette formation and detection of surface immunoglobulins were applied to the comparative study of the peripheral blood lymphocytes populations and tonsils in man. The relative lymphocyte content with different surface characteristics differed significantly in these populations. In detection of surface immunoglobulins on EAC-rosette forming cells it was revealed that not all the C3-lymphocytes of the tonsils bore surface Ig. A possibility of appearance of the C3-receptor on the activated T-lymphocytes is discussed.

[Comparative characteristics of 2 methods of separating mouse T- and B-lymphocytes]. / Sravnitel'naia kharakteristika dvukh metodov razdeleniia T- i B-limfotsitov myshei

The results of separation of T- and B-cells of the C3H/Sn and AKR/j micr lymph nodes with AKR-anti-theta3H and C3H-thetaAKR isosera and cellular immunosorbents containing the antigen-antibody complexes were analyzed in this work. The strain differences in the theta+-cells (T-lymphocytes) level were demonstrated in the untreated mice of the noted strains. Populations enriched by B-lymphocytes under different methods of separation differed somewhat in composition. The cellular immunosorption had many advantages in comparison with the cytotoxic-immune sera used for the T- and B-cell separation.