ABSTRACT
Under action of growth-stimulating concentrations of bioregulator stifun on wheat plants, an increase of functional activity of nucleoli of meristematic cells; contents of lectin (wheat germ agglutinin); and activity of proteinases, tripsin inhibitors, and ATPase activity was established. The pool of free amino acids was increased under bioregulator use. Levels of methionine, phenylalanine, cysteine, lysine, and tyrosine were increased. It is likely that stifun could activate protein biosynthesis in wheat plants.
Subject(s)
Amino Acids/biosynthesis , Meristem/drug effects , Plant Growth Regulators/pharmacology , Seedlings/drug effects , Seeds/drug effects , Triticum/drug effects , Wheat Germ Agglutinins/biosynthesis , Adenosine Triphosphatases/biosynthesis , Adenosine Triphosphatases/metabolism , Dose-Response Relationship, Drug , Meristem/growth & development , Peptide Hydrolases/biosynthesis , Peptide Hydrolases/metabolism , Plant Extracts/chemistry , Plant Extracts/pharmacology , Plant Growth Regulators/chemistry , Plant Proteins/biosynthesis , Secale/chemistry , Seedlings/growth & development , Seeds/growth & development , Triticum/growth & developmentABSTRACT
We studied the pattern of embryo formation in wheat and buckwheat interspecific hybrids. Formation of non-reduced gametes in the embryo sac was induced by low temperature (4-7 degrees C). Changed thermal conditions during meiosis proved to favor formation of hybrid embryos. Vital embryos have been obtained. Karyotypic analysis on the metaphase plates from the root meristem demonstrated different genotypes. Heterogeneous embryos caring both maternal and paternal chromosomes as well as the embryos caring solely maternal or paternal chromosomes have been revealed. Spermatozoa penetrate the embryo sac as a cell, which increases aminopeptidase activity. Sometimes low temperature induces formation of somatic embryos from nucellus cells in the plants.