ABSTRACT
We studied the ability of the polyphenolic complex from Maackia amurensis, the active substance of Maksar, to inhibit the cytopathogenic effect induced by the SARS-CoV-2 and to reduce the concentration of viral RNA in infected Vero E6 cells. Polyphenolic complex showed significant anti-SARS-CoV-2 activity and effectively inhibited viral replication by direct action on viral particles and the early stage of viral infection.
Subject(s)
COVID-19 , SARS-CoV-2 , Animals , Chlorocebus aethiops , Maackia , Vero Cells , Virus Replication , Antiviral Agents/pharmacologyABSTRACT
The quinoid pigment echinochrome A isolated from the sea urchin Scaphechinus mirabilis, the product of its oxidation dehydroechinochrome, and structurally similar antiviral agent oxolin were tested for their ability to inhibit plaque formation induced by herpes simplex virus type 1 (HSV-1) in Vero cells. The tested compounds showed significant anti-HSV-1 activity, mainly due to their direct effect on viral particles and on virus attachment to cells. The antiviral efficacy of the test compounds increased in the following order: oxolinâechinochrome Aâdehydroechinochrome.
Subject(s)
Antiviral Agents/pharmacology , Herpesvirus 1, Human/drug effects , Naphthoquinones/pharmacology , Animals , Chlorocebus aethiops , Herpes Simplex/pathology , Herpes Simplex/prevention & control , Herpes Simplex/virology , Herpesvirus 1, Human/physiology , Naphthoquinones/metabolism , Oxidation-Reduction , Sea Urchins/metabolism , Tetrahydronaphthalenes/pharmacology , Vero Cells , Virus Attachment/drug effects , Virus Internalization/drug effectsABSTRACT
Survival of viruses in the environment is a very important problem in epidemiology, especially for infections with indirect transmission. This work describes the results of the experimental study of adsorption and survival of the hantavirus on different environmental substrates (natural organic and inorganic sorbents). Bovine serum albumin (BSA) solution (5-10%) was effective in the hantavirus elution and phosphate-buffer saline (PBS) pH- 7,2 was optimal for elution of specific RNA. Potential survival of the infectious hantavirus on environmental substrates was observed within up to 14 days at +4°C.
Subject(s)
Orthohantavirus/physiology , RNA, Viral/biosynthesis , Soil Microbiology , Virus Replication/physiology , Adsorption , Animals , Chlorocebus aethiops , Epidemiological Monitoring , Humans , Russia , Serum Albumin, Bovine/chemistry , Vero Cells , Zeolites/chemistryABSTRACT
This paper presents data on the detection of hantavirus in the excretory organs of Apodemus mice which are natural carriers of pathogenic hantaviral serotypes in the Primorye Region. Acute infection periods were detected in naturally infected rodents with the highest hantavirus dissemination to the environment in different phases of rodent population cycles. There was a relationship between the rise of morbidity and the seasonal manifestation of hemorrhagic fever with renal syndrome morbidity and activity of hantavirus reproduction in the murine excretory organs.
