ABSTRACT
The advances in transplantation of hemopoietic stem cells (HSC) from umbilical human blood (UHB) necessitate introduction of effective methods of HSC isolation and preservation. The studies show that UHB cells remained viable even after several days of storage at room temperature. Density gradients proved effective for isolation of cell precursors from UHB. Most of hemopoietic cell-precursors in UHB have low density (under 1.070 g/ml) which becomes greater with cell maturation.
Subject(s)
Fetal Blood/cytology , Hematopoietic Stem Cell Transplantation , Leukocytes, Mononuclear , Blood Cell Count , Evaluation Studies as Topic , Humans , Infant, NewbornABSTRACT
Characteristics of lymphocyte populations in the bone marrow and peripheral blood were comparatively analyzed in children with non-T acute lymphoid leukemia (ALL) during remission, and in the reference group of children with conditionally normal hemopoiesis, as well as with population of lymphocytes obtained from the femur of 22-32-week fetuses. The analysis has shown pronounced changes in the children during remission. The shifts in the structure of lymphocyte population in the bone marrow of children during remission of non-T ALL are considered as compensatory, under conditions of a secondary immunodeficiency due to an abrupt diminution of the absolute number of bone marrow and circulating lymphocytes.