[Study of a plate apparatus for membrane extraction in detoxication of biological fluids]. / Issledovanie raboty tarel'chatogo apparata membrannoi ékstraktsii dlia detoksikatsii biologicheskikh zhidkostei.

The structure of a plate apparatus for detoxication of biological fluids has been described. The operation of the apparatus for cholesterol recovery from blood and artificial biological fluid by the extracting emulsions "water in oil" has been investigated. The parameters of the apparatus, such as the number of plates, the height of the apparatus, the volume of extracting emulsion, have been determined. The blood flow velocity which excludes the entry of extracting emulsion drops into biological fluid has been chosen. The values of cholesterol recovery (22.4%) and of red blood cell hemolysis when blood is present in the working apparatus chamber have been defined.

[Study of the mode of operation of the equipment for membrane extraction of substances from the blood in emulsions]. / Issledovanie rezhimov raboty apparata dlia membrannoi ékstraktsii vo mnozhestvennoi ému'lsii iz krovi.

The authors suggest an apparatus make-up for cholesterol extraction in multiple emulsion from blood. It is intended for homeostasis correction according to the hemoperfusion scheme with the use of this apparatus instead of the hemoperfusion one. The influence of the velocity of the blood flow, the rate of stirring and emulsion volume on the modes of operation of the apparatus was investigated. The most efficient mode of operation was chosen, according to which the entry of the extracting agent into the body was completely excluded whereas emulsion saturation with cholesterol was reached after 1.5 h. A series of animal experiments were carried out using the same apparatus but 10 times smaller, with the emulsion containing ethanol and diethyl ether in the internal phase. The mean degree of cholesterol extraction amounted to 22.4%, while the main biochemical components of blood remained unchanged.

[Extraction of cholesterol from the blood using multiple emulsions]. / Ekstraktsiia kholesterina iz krovi krolika vo mnozhestvennye émul'sii.

The process of cholesterol extraction from the blood of rabbits with experimental atherosclerosis was investigated, with the following reinjection of the blood with reduced amount of cholesterol. Liquid membranes were applied to extract free cholesterol. Emulsion consisting of blood-compatible membrane (oil and sorbitan oleate) and the inner phase (water solution of digitonin) was used as an extractant. The average degree of cholesterol extraction was 23.4%. Pretreatment of the emulsion, its composition and the way of preparation make it possible to perform the extraction from the blood without a considerable increase in the degree of hemolysis.

[Extraction of lipoprotein components with emulsions]. / Izvlechenie komponentov lipoproteidov émul'siiami.

Extraction of lipoprotein components from water solutions by means of emulsions similar to "water in oil" was studied, where either suspension of glycosides in buffer or water solution of diethyl ether and ethanol were used as an inner phase of the emulsion. The first type of extraction enabled to isolate selectively free cholesterol from lipoproteins and the second type--lipids, respectively. The rate of cholesterol isolation from lipoproteins of various classes, dependent on the strength of cholesterol binding in lipoproteins was increased as follows: alpha less than beta less than pre-beta-lipoproteins; the procedure allowed to isolate selectively cholesterol from atherogenous lipoproteins.