ABSTRACT
Changes in fluorescence intensity of blood lymphocytes of patients with somatic and pretumorous diseases of the gastrointestinal system were detected: fluorescence intensity of lymphocytes fluorochrome-stained with acridine orange was increased in chronic gastric and pancreatitis whereas in patients with pretumorous diseases this parameter could be both increased and decreased. Exposure to an extra stress factor, such as low-dose ionizing radiation, manifested by increased fluorescence intensity of normal subjects' blood lymphocytes, a certain normalization of this parameter in patients with somatic diseases, and an increase of the amplitude of shifts vs. the normal range in patients with pretumor gastrointestinal diseases. Fluorescent analysis of lymphocytes helps integrally assess the functional activity of immunohomeostasis.
Subject(s)
Fluorescence , Gastrointestinal Diseases/blood , Lymphocytes , Acridine Orange , Adult , Female , Gastrointestinal Neoplasms/blood , Humans , Lymphocytes/radiation effects , Male , Middle Aged , Precancerous Conditions/bloodABSTRACT
The authors demonstrate the possibility of using the fluorescent analysis of the peripheral blood lymphocytes for the detection of the specific sensitization of the body to allergens and of immune deficiency. A positive correlation has been revealed between intensified fluorescence (at 640 nm) of blood lymphocytes stained with fluorochrome acridine orange, in vitro stimulated with a specific allergen, and the blood serum antihapten humoral antibodies, as well as deviations of some other laboratory parameters from the norm. High informative value of the fluorescent analysis, use of small quantities of blood recommend this method for screening examinations and formation of groups at risk of developing occupational diseases.
Subject(s)
Allergens/immunology , Hypersensitivity/immunology , Luminescent Measurements , Lymphocytes , Occupational Diseases/immunology , Adult , Animals , Guinea Pigs , Humans , Hypersensitivity/blood , Male , Middle Aged , Models, Biological , Occupational Diseases/blood , Risk FactorsABSTRACT
The author emphasizes the informative value and high sensitivity of fluorescent analysis of blood lymphocytes, fluorochromium-treated with acridine orange, to changes in the body status. Use of blood microvolumes makes this method promising for screenings of the population and for monitoring the patients.
Subject(s)
Health Status , Lymphocytes/pathology , Microscopy, Fluorescence , Population Surveillance/methods , Acridine Orange , Adolescent , Adult , Aged , Child , Child, Preschool , Female , Humans , Male , Middle AgedABSTRACT
Heparin treatment of the blood changes the peripheral blood lymphocyte chromatin nuclear structure inducing a dissociation of the nucleoprotein complexes; this fact should be borne in mind when examining the white blood cellular functional activity and in cytodiagnostic investigations.
