[Discovery and quantitative determination of intestinal viruses by the detection of viral antigens in infected cells using an immunoperoxidase method]. / Obnaruzhenie i kolichestvennoe opredelenie kishechnykh virusov s pomoshch'iu detektsii virusnykh antigenov v zarazhennykh kletkakh immunoperoksidaznym metodom.

The use of immunoperoxidase method for detection of virus antigens in a fixed infected cell monolayer on microplates (cellular immunoperoxidase method, CIPM) for poliomyelitis and hepatitis A virus as well as for human and simian SA II rotaviruses is described, as well as the method of quantitation of rotaviruses by counting of virus multiplication foci. The CIPM combines the advantages of the methods based on the infectivity determinations and of immunochemical methods, and may be used both for diagnosis and for detection and quantitation of enteric viruses.

Peculiarities of tick-borne encephalitis virus reproduction in Haemaphysalis inermis ticks and their explants.

Virological, electron microscopic and immunomorphological investigation on the reproduction of tick-borne encephalitis (TBE) virus in Haemaphysalis inermis ticks and in tissue explants from nymphs during their metamorphosis revealed that the virus reproduced in the cells of different tissues and organs of ticks which were in different phases and stages of life cycle. During the eclipse phase of TBE virus persistence in ticks, when no virus could be demonstrated by infectivity assay, viral particles were detected by electron microscopy.