Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
FEBS Lett ; 583(4): 831-4, 2009 Feb 18.
Article in English | MEDLINE | ID: mdl-19187779

ABSTRACT

Epidemiological evidence suggests a link between chronic oxygen starvation and fat accumulation/obesity, however the underlying mechanism remains unclear. Using Caenorhabditis elegans we found extended oxygen deprivation resulted in activation of SBP-1, the worm homologue of SREBP1, a transcription factor important in maintaining lipid homeostasis. SBP-1 knockdown prevented hypoxia-induced fat accumulation and the associated increase in worm width/length ratio, demonstrating that SBP-1/SREBP1 plays an essential role in hypoxia-induced lipid accumulation and body shape alteration. This study provides the first evidence suggesting that activation of SREBP1 may be a critical pathogenic factor contributing to chronic hypoxia associated excessive fat accumulation/obesity in humans.


Subject(s)
Body Size , Caenorhabditis elegans Proteins/physiology , Caenorhabditis elegans/physiology , Lipids/biosynthesis , Oxygen/metabolism , Transcription Factors/physiology , Animals , Caenorhabditis elegans/genetics , Caenorhabditis elegans/metabolism , Caenorhabditis elegans Proteins/genetics , Caenorhabditis elegans Proteins/metabolism , Lipids/analysis , RNA Interference , Transcription Factors/genetics , Transcription Factors/metabolism
2.
J Pharm Biomed Anal ; 49(2): 319-26, 2009 Feb 20.
Article in English | MEDLINE | ID: mdl-19155153

ABSTRACT

Oversulfated chondroitin sulfate (OSCS), an impurity found in some porcine intestinal heparin samples was separated from intact heparin by capillary electrophoresis (CE) using a 600mM phosphate buffer, pH 3.5 as the background electrolyte in a 56cm x 25microm i.d. capillary. This method was confirmed in two separate labs, was shown to be linear, reproducible, robust, easy to use and provided the highest resolution and superior limits of detection compared to other available CE methods. Glycosoaminoglycans such as dermatan sulfate and heparan sulfate were separated and quantified as well during a single run. The heparin peak area response correlated well to values obtained using the official assay for biological activity. A high speed, high resolution version of the method was developed using 600mM lithium phosphate, pH 2.8 in a 21.5cm x 25microm i.d. capillary which provided limits of detection for OSCS that were below 0.1%.


Subject(s)
Anticoagulants/chemistry , Chondroitin Sulfates/chemistry , Dermatan Sulfate/chemistry , Drug Contamination , Heparin/chemistry , Heparitin Sulfate/chemistry , Phosphates/chemistry , Animals , Anticoagulants/analysis , Buffers , Calibration , Chondroitin Sulfates/analysis , Chondroitin Sulfates/isolation & purification , Dermatan Sulfate/analysis , Dermatan Sulfate/isolation & purification , Electrolytes/chemistry , Electrophoresis, Capillary/instrumentation , Electrophoresis, Capillary/methods , Heparin/analysis , Heparitin Sulfate/analysis , Heparitin Sulfate/isolation & purification , Hydrogen-Ion Concentration , Molecular Structure , Osmolar Concentration , Reproducibility of Results , Sensitivity and Specificity , Sus scrofa , Temperature , Time Factors
SELECTION OF CITATIONS
SEARCH DETAIL
...