[The relief characteristics of the apical cell surface of endometrial epitheliocytes in women suffering from sterility]. / Osobennosti rel'efa apikal'noi kletochnoi poverkhnosti épiteliotsitov éndometriia zhenshchin, stradaiushchikh besplodiem.

Functional changes in the surface topography of the uterus in women of reproductive age have been studied using scanning electron microscopy. The state of the cell surface relief was estimated at different phases of the menstrual cycle and in sterile women. The presence of wide and short ruffles of epithelial cells covered with mucus is typical of the secretory phase of the cycle. No microvilli or cilia are seen on the epitheliocyte surface. In contrast, the follicular phase is characterized with numerous deep ruffles of epithelium covered with cylindrical cells. The apical surface is studded with numerous microvilli and cilia persisting even in sterile women at all the stages of the cycle. The disturbances of nidation processes in the fertilized ovule may apparently be associated with the patterns of the cell surface relief.

[Human extracorporeal fertilization with subsequent successful embryo implantation and the birth of a child]. / Ekstrakorpoal'noe oplodotvorenie u cheloveka s poslediushchei uspeshnoi implantatsiei émbriona i rozhdeniem rebenka.

With the aim to use a previously elaborated method of extracorporeal fertilization of human ova for treating sterility, resulted from obstruction or absence of the uterine tubes, ova were obtained at laparoscopy after a previous hormonal stimulation of women. From 119 women 159 ova were obtained. Fertilization of the ova was performed in the medium Ham F-10 previously capacitated with the husband's spermatozoa. Fertilization rate was 66%. Embryos at the stage of 2-8 blastomeres were implanted transcervically into the uterine cavity with a special catheter. The embryo transfer was performed in 37 women with tubular infecundity. Menoschesis up to 3 weeks was noted in 5 women. In one woman on the 35th day after implantation, by means of hormonal-biological reaction and ultrasound investigation, pregnancy was diagnosed, it terminated in timely birth of a boy with body mass 3,500 gr. A conclusion was made that the main cause of failures at the embryo transfer was an inadequate preparation of the uterine mucosa (resulted from the main disease) to accept the fertilized ovum.