Effect of combined treatment with aspirin and dipyridamole on oxidative homeostasis in mouse serum.

Using the method of peroxidative luminol-dependent chemiluminescence we showed that combined treatment with aspirin in low dose and dipyridamole corrects imbalance in oxidative homeostasis in mouse serum. This state resulted from a sharp increase in prooxidant processes after platelet disaggregation with normal dose of aspirin or dose-dependent inhibition of free radical processes with dipyridamole.

Effect of imbalance in plasma oxidant homeostasis on the choice of stromal differentiation of polypotent bone marrow stem cells in mice receiving estrone injection.

The choice of stromal differentiation by polypotent stem cells was studied by morphological and chemiluminescent methods. The differentiation pathway is selected on day 7 after heterotopic bone marrow transplantation and injection of estrone. The imbalance in plasma oxidant homeostasis in mice on day 7 after estrone injection is associated with drastic intensification of free-radical oxidation and blockade of antioxidant activity.

Effect of disturbed plasma oxidant homeostasis on hemopoietic differentiation of polypotent bone marrow stem cells in mice treated with dipyridamole.

The choice of hemopoietic differentiation by polypotent bone marrow stem cells was studied by morphological and chemiluminescent methods. This choice occurred on day 7 after heterotopic bone marrow transplantation and dipyridamole administration, under the effect of oxidative homeostasis imbalance in the plasma. Dipyridamole increased total antioxidant activity and 10-fold reduced the intensity of free radical oxidation. Disaggregation therapy with dipyridamole reduced platelet count in the peripheral blood and decreased total plasma antioxidant activity.

Free radical oxidation and antioxidant activity in blood plasma and myocardium during long-term dipyridamole treatment.

Activation of free radical oxidation and inhibition of antioxidant activity in mouse myocardium after long-term dipyridamole treatment were demonstrated using chemiluminescent techniques. At the same time, dipyridamole 10-fold inhibited free radical oxidation and slightly increased antioxidant activity in the plasma. Dipyridamole-induced platelet disaggregation was accompanied by an increase in platelet count.

[A clinical and experimental validation of the use of Stimuloss in patients with chronic generalized periodontitis]. / Kliniko-éksperimental'noe obosnovanie primeneniia u bol'nykh khronicheskim parodontitom "Stimulossa".

The pharmacological effect of hydroxyapatite and a composition based on it in the form of Stimulos sponge has been studied in human osteogenic tissue culture. Hydroxyapatite stimulated the biosynthetic processes in the cells. Addition of chlorohexidine and thymogen to the composition in order to increase its antibacterial activity did not decrease the osteoinductive properties of the drug. Trials of Stimulos in 66 patients with chronic generalized periodontitis demonstrated its high efficacy, particularly of the compositions containing immunostimulants: inflammation disappeared and the height of alveolar osseous tissue increased, as did bone compactness.

[The regulation of bone marrow hematopoiesis in splenectomy and splenic autotransplantation in mice]. / Reguliatsiia kostnomozgovogo krovetvoreniia pri splenéktomii i autotransplantatsii selezenki u myshei.

The method of heterotopic transplantation of the bone marrow was used to study the influence of splenectomy and autotransplantation of the spleen onto the hemopoietic and osteogenic tissues of the bone marrow in mice. After splenectomy at early stages of the formation of the heterotopic hemopoiesis focus the amount of myelokaryocytes increased while in the stationary bone marrow population (the femur bone) their amount dropped by the same time. Autotransplantation of the spleen made the amount of myelokaryocytes equal to the level of control, but did not eliminate the effect of decrease of the mass of osteogenic tissue in the heterotopic hemopoiesis focus arising after splenectomy, which might be related with the influence of splenectomy on the stem osteogenic cells-precursors of the bone marrow.

[The action of dipyridamole on megakaryocytopoiesis in regenerating and stationary bone marrow cell populations]. / Deistvie dipiridamola na megakariotsitopoéz v regeneriruiushchei i statsionarnoi populiatsiiakh kletok kostnogo mozga.

The effect of dipyridamole on megakaryocytopoiesis in regenerating and stationary populations of mouse bone marrow cells has been studied by heterotopic transplantation of the bone marrow using histological, electron microscopic and biochemical techniques. It is shown that drug administration induced destruction of megakaryocytes. In megakaryocytic cytoplasm giant lipid granules were found whose growth and number increase resulted in megakaryocytes kill. Gas-liquid chromatography was used to evaluate the effect of dipyridamole on distribution of lipid fatty acids of the stationary and regenerating populations of the bone marrow cells. A marked increase of the percentage of docosahexaenoic acid was found in lipids of the stationary population. Chronic dipyridamole administration caused an increase of percentage of myristic, palmitic oleic acids, and decrease of percentage of arachidonic and eicosapentaenoic acids in lipids of regenerating bone marrow cells population.

[The effect of hydroxylapatite on the experimental healing of the tooth socket]. / Vliianie gidroksilapatita na zazhivlenie lunki zuba v éksperimente.

Synthetic hydroxylapatite, a drug made in this country, enhanced reparative osteogenesis if introduced in the dental well of a white rat. The regenerate represented solid bone structure in 4 months after tooth removal.

[Compensation-adaptation reactions of hemopoiesis-inducing microenvironment of bone marrow in stress]. / Kompensatorno-prisposobitel'nye reaktsii gemopoézindutsiruiushchego mikro-okruzheniia kostnogo mozga pri stresse.

The time course of compensation-adaptation reactions of bone marrow hemopoiesis-inducing microenvironment (HIM) was studied in F1 (CBA x C57BL/6) mice subjected to 10-hour immobilization. The number of macrophage and fibroblastoid colony-forming units, committed and stem precursor stroma cells responsible for HIM transport was shown to increase in the hemopoietic tissue during adaptation syndrome. HIM activation in stress was found to be macrophage- and lymphocyte-dependent process.

[Effect of dipyridamole on the interrelations of the stromal and hematopoietic tissues of the bone marrow in experimental studies]. / Deistvie dipiridamola na vzaimootnosheniia stromy i gemopoieticheskoi tkani kostnogo mozga v éksperimente.

By means of heterotopic transplantation of the bone marrow interrelations of the stromal and hemopoietic tissues of the mice bone marrow have been studied at administration of dipiridamol. Effect of the drug to the hemopoiesis is realized via stem stromal cells of the bone marrow. Under the influence of dipiridamol a focus of heterotopic hemopoiesis the osteogenic component in it is present only in 30% of cases in comparison with the control. Inhibition of the stromal component proliferation is accompanied with increasing mitotic activity of the hemopoietic elements against the background of the bone marrow cellularity decrease both in the femoral bone and in the focus of heterotopic hemopoiesis. At administration of dipiridamol a phenomenon of noneffective megakaryocytopoiesis with the intrabone marrow destruction of megakaryocytes, resulting in local release of thrombocyte growth factor, which has a compensatory character.

[Role of stromal microenvironment in the regulation of bone marrow hemopoiesis after curantyl administration]. / Izuchenie roli stromal'nogo mikrookruzheniia v reguliatsii kostnomozgovogo gemopoéza pri vvedenii kurantila.

Role of the stromal microenvironment in regulation of bone marrow hemopoiesis at the administration of the thrombocyte disaggregant curantyl was studied by the method of heterotopic transplantation of the mice bone marrow. It is shown that the action of curantyl on hemopoiesis is realised through the stem stromal cells of the bone marrow. It is noted that the inhibitory action of the preparation on proliferation of osteogenic precursor-cells is followed by activation of bone resorption processes in regenerating ectopic hemopoietic organ. Under the action of curantyl at low bone marrow cellularity in the focus of heterotopic hemopoiesis and femur an increase of mitotic activity in hemopoietic elements is noted. It is revealed that a phenomenon of ineffective megakaryocytopoiesis with intramedullary destruction of megakaryocytes leads to the local excretion of the thrombocyte released growth factor (TRGF) which has a compensatory character.

[The role of the thymus in regulating the stromal cells responsible for the transfer of the hemopoiesis-inducing microenvironment in stress]. / Rol' timusa v reguliatsii kletok stromy, otvetstvennykh za perenos gemopoézindutsiriushchego mikrookruzheniia pri stresse.

The role of thymus in the regulation of stromal elements, responsible for haemopoiesis inducing microsurrounding transfer of animals, subjected to 10-hours immobilization, was studied. The development of bone marrow hyperplasia and stimulation of functional activity of stromal cells, responsible for haemopoiesis inducing microsurrounding transfer, were shown to be thymus dependent processes during stress.

[Interrelation of the osteogenic and hemopoietic tissues of the bone marrow during the development of estrogenic myelofibrosis]. / Vzaimootnosheniia osteogennoi i gemopoéticheskoi tkanei kostnogo mozga v protsesse razvitiia éstrogenovogo mielofibroza.

By means of estrogenic myelofibrosis, using the method of heterotopic transplantation of the bone marrow, interrelations of osteogenic and hemopoietic tissues have been studied. Under estrone effect disorders in stromal microenvironment take place at the level of stem osteogenic cells of the bone marrow. Deficiency in cells of monocytic-macrophagal line, inhibiting proliferation of the bone marrow connective tissue cells, contributes to development of myelofibrosis. The hormone acts mainly in committed hemopoietic cells, singly launching them from G0- into S-period of the cycle, and then--into differentiation, accompanied with an enhanced discharge of cells from the bone marrow. There is neither direct, nor indirect dependence between the osteogenic mass and the cellularity of the hemopoietic tissue of the bone marrow. The changes, that take place in the bone marrow under estrone effect, are reversible.

[The role of glycosaminoglycans in regulation of medullary hematopoiesis in stress]. / Rol' glikozaminoglikanov v reguliatsii kostnomozgovogo krovetvoreniia pri stresse.

The role of the constituents of hematopoiesis inducing bone marrow microenvironment in myelopoiesis regulation was studied. It was found that during the development of the general adaptation syndrome the functional activity of stroma cells and granulomonocytopoiesis precursor cells intensifies and the content of acid glycosaminoglycans in the bone marrow increases. It is shown that acid glycosaminoglycans produce an effect on the myelokaryocytes in intimate interrelationship with the prostaglandin system, changing the level of intracellular calcium and cAMP in the hematopoietic cells.

[Effect of a deficiency in the mononuclear phagocyte system and the administration of yeast polysaccharide on the development of a heterotopic source of hematopoiesis]. / Vliianie defitsita sistemy mononuklearnykh fagotsitov i vvedeniia drozhzhevogo polisakharida na postroenie geterotopnogo ochaga krovetvoreniia.

Mononuclear phagocyte system (MPS) deficiency was induced by repeated peritoneal lavage in (C57Bl x CBA) F1 mice. The animals were then used as donors or recipients in heterotopic bone marrow transplantation. Yeast polysaccharide (YP) produced by Cryptococcus luteolus strain 228 was injected weekly (25 mg/kg) during 30 days after bone marrow transplantation under the kidney capsule. Bone marrow transplantation from MPC-deficient mice to intact mice 30 days later resulted in no variations from the control in cellularity and ossicle weight. YP produced an increase in cellularity, but not in ossicle weight. In the opposite experimental scheme (transplantation from intact mice to MPS-deficient mice) an increase in both cellularity and weight was not noticed. YP injections in this case resulted in the reduction of heterotopic organ size to the control level. Possible mechanisms of this phenomenon are discussed.

[Role of the stromal microenvironment in regulating bone marrow hemopoiesis under stress]. / Rol' stromal'nogo mikrookruzheniia v reguliatsii kostnomozgovogo krovetvoreniia pri stresse.

The role of stromal microenvironment in bone marrow hemopoiesis during stress has been studied. The stimulation of functional activity of stromal cells, responsible for the transfer of hemopoiesis-inducing microenvironment has been found. The time required for the development of microenvironment coincide with the moment of T-lymphocyte appearance in the bone marrow. The induction of microenvironment preceded the development of hemopoiesis hyperplasia at the level of committed precursor cells of myelopoiesis and their more mature, morphologically differentiated progeny.

[Formation of a heterotopic hemopoiesis focus from the administration of a cryptococcal heteropolysaccharide to bone marrow donors and recipients]. / Postroenie geterotopnogo ochaga krovetvoreniia v usloviiakh vvedeniia donoram i retsipientam kostnogo mozga geteropolisakharida kriptokokkov.

The effect of Cryptococcus luteolus (strain 228) heteropolysaccharide on heterotopic bone marrow organ formation was studied. Heteropolysaccharide (Hp) injections were initiated on the next day after bone marrow transplantation and were performed weekly for 30 days. This resulted in the increase in both the cellularity and the weight of the ossicle. The injections performed twice weekly produced the decrease of cellularity while ossicle weight did not change. Higher dose of Hp produced no changes in heterotopic bone marrow organ. Hp injections to donor mice did not result in any changes in heterotopic organ in bone marrow recipients. Thus, this kind of Hp was capable both of stimulating and inhibiting heterotopic bone marrow organ formation in relation to the dose and scheme of administration.

[Effect of estrone on formation of the focus of heterotopic hematopoiesis]. / Vliianie éstrona na obrazovanie ochaga geterotopnogo krovetvoreniia.

The effect of estrone injections on the heterotopic bone marrow organ formation was studied in (C57Bl X CBA)F1 mice by bone marrow shaft transplantation under the kidney capsule. Estrone injections resulted in femur marrow fibrosis, the increase in the weight of femur and heterotopic organ ossicle, drastic reduction in heterotopic organ cellularity. Depression of hemopoiesis in ectopic organ cannot be attributed to mechanical displacement of hemopoietic cells because a newly formed bone cavity under the kidney capsule was not closed. Thus, estrone had a detrimental effect on the creation of microenvironment in the heterotopic organ.