ABSTRACT
AIM: Study the prevalence and mechanisms of resistance in circulating C. diphtheriae strains. MATERIALS AND METHODS: 664 C. diphtheriae strains isolated in 1987 - 2013 in various regions of Russia and sent to the reference center of Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology were the object of the study. Antibiotic sensitivity of the strains was studied by disk-diffusion and E-test methods using 10 antimicrobial preparations. Nucleotide sequence analysis was carried out by using BLAST program and EMBL/GenBank database. RESULTS: Most of the studied strains turned out to be sensitive to all the antibacterial preparations used. 1.2% of C. diphtheriae strains turned out to be resistant to penicillin and 6.0% had intermediate level of resistance. 0.4 - 0.6% of the strains had intermediate level of resistance to macrolides, and 4.0 - 4.4% were resistant. 2.0% of the strains had multiple resistance. Erm(X)-specific PCR carried out in this study showed that all the C. diphtheriae strains resistant to macrolide antibiotics carry erm(X) gene. CONCLUSION: The results of the study indicate a fairly high level of prevalence for C. diphtheriae strains resistant to antibiotics.
Subject(s)
Anti-Bacterial Agents/administration & dosage , Corynebacterium diphtheriae/drug effects , Diphtheria/drug therapy , Drug Resistance, Bacterial/genetics , Corynebacterium diphtheriae/classification , Corynebacterium diphtheriae/pathogenicity , Humans , Macrolides/administration & dosage , Microbial Sensitivity Tests , RussiaABSTRACT
The article deals with the results of studying the growth characteristics of nine nutrient mediums for primary plating of pathologic material. It is demonstrated that for successful and proper functioning of bacteriologic laboratory providing bacterial analysis for diphtheria the permanent quality control is needed to monitor the nutrient mediums for primary plating. The quality control is applied to evaluate the growth characteristics on such criteria as germination of isolated culture, intensity of its growth in 24 and 48 hours, optimal size of colonies characterized by their cultural characteristics, inhibiting activity concerning concurrent microflora.
Subject(s)
Bacterial Typing Techniques/standards , Corynebacterium diphtheriae , Culture Media/standards , Diphtheria , Bacterial Typing Techniques/methods , Corynebacterium diphtheriae/classification , Corynebacterium diphtheriae/growth & development , Corynebacterium diphtheriae/isolation & purification , Diphtheria/diagnosis , Diphtheria/microbiology , Female , Humans , Male , Quality ControlABSTRACT
AIM: Genotyping of B. pertussis strains isolated from pertussis patients in Moscow. MATERIALS AND METHODS: 53 strains of B. pertussis isolated from pertussis patients in Moscow in 2007 - 2010 as well as 3 vaccine strains currently used in Russia for the production of DTP vaccine were studied by multilocus sequencing (MLST) based on allele combinations of ptxA, ptxC and tcfA genes. RESULTS: A genetic characteristic of B. pertussis strains isolated from pertussis patients in Moscow by using MLST is presented. Allele profile analysis of the studied B. pertussis strains was performed, 4 sequence types (ST) were identified--ST1, ST2, ST3 and ST5, most of the circulating strains (86.7%) were shown to belong to ST5, equal percentage of cases (5.7%)--to ST2 and ST3, and 1.9%--to ST1, while 2 vaccine production strains belong to ST2 and 1 - to ST1. CONCLUSION: Clonal structure of contemporary Moscow strains was shown to be different from strain structure used for the production of DTP vaccine.
