Subject(s)
Brain/physiology , Conditioning, Classical/physiology , Gene Expression Regulation/physiology , Proto-Oncogenes/physiology , Animals , Escape Reaction/physiology , Feeding Behavior/physiology , Male , Motor Activity/physiology , Nucleic Acid Hybridization , RNA/genetics , Rats , Time FactorsABSTRACT
Expression of the c-fos proto-oncogene has been associated with mitosis or differentiation in a number of cultured cells or tissues in vivo. Expression of the c-fos proto-oncogene in adult rat brain cells was studied in the process of learning with the use of Northern hybridization techniques. Our results demonstrate that high levels of c-fos mRNA are detectable in brains of all animals treated. Therefore, c-fos is likely to play an important role in the process of learning.
Subject(s)
Cerebral Cortex/analysis , Gene Expression , Protein-Tyrosine Kinases/genetics , Proto-Oncogene Proteins/genetics , Proto-Oncogenes , Animals , Blotting, Northern , Cerebral Cortex/radiation effects , Male , Nucleic Acid Hybridization , Proto-Oncogene Proteins c-fos , RNA, Messenger/analysis , RatsABSTRACT
Cardiac muscle proteins of four inbred murine strains were analyzed by one- and two-dimensional electrophoresis. Of those, 27 and 161 protein fractions were characterized in terms of molecular mass and relative electrophoretic mobility. The protein fractions were identified as corresponding to creatine phosphokinase, myoglobin and an albumin-like protein. Six polypeptides characterized by interlinear polymorphism were identified.
