Subject(s)
Rubella/diagnosis , Serologic Tests , Adult , Antibodies, Viral/analysis , Complement Fixation Tests , Female , Hemagglutination Inhibition Tests , Humans , Immunoglobulin G/analysis , Immunoglobulin M/analysis , Infant, Newborn , Maternal-Fetal Exchange , Neutralization Tests , Pregnancy , Rubella/congenital , Rubella/immunology , Rubella/prevention & control , Rubella Vaccine , Rubella virus/immunology , Time Factors , VaccinationSubject(s)
Antibodies , Rubella virus/immunology , Viral Vaccines , Child , Humans , Methods , Rubella Vaccine , School Health Services , VaccinationABSTRACT
The efficacy of an attenuated rubella virus vaccine, Cendevax, was tested on 65 school children. Forty-nine of them (75%) had pre-existing antibodies and in these there was no increase in the HAI antibody titres after administration of the vaccine. Sixteen children (25%) had no demonstrable rubella HAI antibody prior to vaccination. From the latter group, postvaccination serum samples were available from only 11, and 10 of these seronegative children showed seroconversion after vaccination. The geometric mean HAI titre was 1:180. Seven of the 10 postvaccination serum samples had complement-fixing antibodies and specific IgM antibodies were detected by the immunofluorescence test in 8. No correlation was observed between the CF and the IgM antibodies.
Subject(s)
Antibodies/analysis , Rubella virus , Viral Vaccines , Child , Complement Fixation Tests , Female , Fluorescent Antibody Technique , Hemagglutination Inhibition Tests , Humans , Immunoglobulin M/analysis , Injections, Subcutaneous , Male , Pharynx/microbiology , Rubella/immunology , Rubella Vaccine/administration & dosage , Rubella virus/isolation & purificationABSTRACT
The applicability of the immunofluorescence (IF) test to the diagnosis of primary rubella infection was investigated. The test is based on the detection of rubella-specific antibodies in the IgM fraction of immunoglobulins. The results indicate the usefulness of the IF test for the diagnosis of primary rubella infection on a single serum specimen collected at a proper time. The test is also of value in the differentiation of primary infection from reinfection, since in reinfection no rubella-specific antibodies are found in the IgM fraction. The test is also valuable for the detection of fetal infection in utero since the persistence of IgM antibodies in pregnant women is indicative of fetal infection.