ABSTRACT
Nodular fasciitis rarely arises in the hand. We describe 4 cases that were histologically diagnosed as nodular fasciitis on biopsy specimens arising in the hands. The masses of 2 patients were excised due to rapid growth. Both of these patients had no recurrence. The other 2 patients were followed without surgical excision, and the masses partially regressed without functional impairment.
Subject(s)
Fasciitis/diagnosis , Fingers/pathology , Hand/pathology , Adult , Biopsy, Needle , Fasciitis/rehabilitation , Fasciitis/surgery , Female , Follow-Up Studies , Humans , Immunohistochemistry , Magnetic Resonance Imaging/methods , Male , Middle Aged , Sampling Studies , Treatment OutcomeABSTRACT
Increasing attention has been given to vascular tissue engineering in recent years. Although cell seeding onto tubular scaffolds is the first step for constructing three-dimensional vascular grafts, the tubular geometry of the grafts hinders the efficient delivery of cells onto the scaffold. To overcome these limitations, we present here a novel cell-seeding technique using magnetic force and magnetite nanoparticles, termed Mag-seeding. NIH/3T3 fibroblasts (3T3s) were labeled magnetically using our original magnetite cationic liposomes (MCLs), which have a positive surface charge, to improve adsorption onto cell surface. In this study, porcine decellularized common carotid artery (dCCA) was used as one of the most promising scaffolds, because dCCA consists of a mixture of structural and functional proteins that constitute the extracellular matrix. When a cylindrical magnet was inserted into the lumen of dCCA and the dCCA was immersed into a suspension of magnetically labeled 3T3s, almost all the 3T3s attached onto the dCCA, whereas a low cell-seeding efficiency was achieved without using a magnet. When the magnetite uptake rate per cell increased, cell-seeding efficiency by Mag-seeding was enhanced. Furthermore, to construct a vascular graft for humans, the porcine dCCA, which was reseeded with two human cells (smooth muscle cells and dermal fibroblasts), was successfully constructed by Mag-seeding. These results indicate that Mag-seeding can be used for vascular tissue engineering.
Subject(s)
Blood Vessel Prosthesis , Blood Vessels/cytology , Blood Vessels/growth & development , Cell Culture Techniques/methods , Ferrosoferric Oxide/metabolism , Micromanipulation/methods , Tissue Engineering/methods , Animals , Cell Adhesion , Cell Proliferation , Magnetics , Mice , NIH 3T3 Cells , Stress, MechanicalABSTRACT
Thirty-eight patients with advanced Kienböck's disease treated by limited wrist arthrodesis (LWA: n = 10) or radial osteotomy (RO: n = 28) for a fragmented lunate were retrospectively examined after an average of 47.9 and 68.1 months, respectively. Compared with pre-operative values, the active flexion-extension range of motion decreased by about 16.0 degrees in LWA and increased approximately 9.7 degrees in RO and the grip strength improved by approximately 7.5 kg in LWA and 8.0 kg in RO. In both groups, radiographs showed no significant progression of carpal collapse. Although LWA caused some decrease in wrist flexion-extension, both procedures are appropriate for surgical treatment of advanced Kienböck's disease. Most patients experienced a reduction in pain and were able to return to work.
Subject(s)
Arthrodesis , Lunate Bone/surgery , Osteonecrosis/surgery , Osteotomy , Radius/surgery , Adolescent , Adult , Aged , Female , Follow-Up Studies , Humans , Male , Middle Aged , Osteonecrosis/diagnostic imaging , Radiography , Retrospective Studies , Treatment OutcomeABSTRACT
Long-term denervation of muscles results in fibrosis and fat replacement, which prevent muscles from regaining contractile function despite reinnervation. Therefore, prevention of muscle atrophy between nerve repair and muscle reinnervation may improve the functional outcome. A variety of growth factors play significant roles in muscle mass modulation and muscle regeneration. The purpose of the present study was to investigate the effect of fibroblast growth factor-2 (FGF2) and nerve growth factor (NGF) on muscle mass modulation after denervation and reinnervation using a nerve-to-muscle neurotization model. Growth factors were injected into the anterior tibial muscle after direct neurotization of muscles every 7 days up to 4 weeks after surgery. FGF2 significantly increased the amplitude of compound muscle action potentials (CMAPs), wet muscle weight, and the number of motor endplates, especially at higher concentration, compared to the vehicle. In contrast, NGF did not increase either the amplitude of CMAPs or muscle weight, although it significantly increased the number of motor endplates. These findings indicate that both growth factors enhance reinnervation of muscles; however, only FGF2 is involved in muscle mass modulation.
