Molecular regulation, breed differences and genes involved in stress control in farm animals.

Stress is a state of disturbed homeostasis evoking a multiplicity of somatic and mental adaptive reactions resulting from any of the 5 freedoms of animals being violated. Many environmental forces disrupt homeostasis in farm animals, such as extreme temperatures, poor nutrition, noise, hunger, and thirst. During stressful situations, neuronal circuits in the limbic system and prefrontal cortex are activated, which lead to the release of adrenalin and noradrenalin. The hormones released during stress are needed for adaptation to acute stress and are regulated by many genes. This review examined molecular regulation, breed differences, and genes involved in stress control in farm animals. Major molecular regulation of stress, such as oxidative, cytosolic heat shock, unfolded protein, and hypoxic responses, were discussed. The responses of various poultry, ruminant, and pig breeds to different stress types were also discussed. Gene expressions and polymorphisms in the neuroendocrine and neurotransmitter pathways were also elucidated. The information obtained from this review will help farmers mitigate stress in farm animals through appropriate breed and gene-assisted selection. Also, information obtained from this review will add to the field of stress genetics since stress is a serious welfare issue in farm animals.

Is an increase of glucocorticoid concentrations related to the degree of arousal or valence experienced by an animal to a stimulus?

Animal welfare is the quality of life as perceived by the animal itself. It is also the state of an animal in its attempt to cope with its environment. Animal welfare has high ethics and economic importance. Thus the need to develop parameters for assessing animal welfare. An acute increase in glucocorticoid (GC) concentration is necessary for adaptation to a stressful situation. Glucocorticoids also play a significant role in metabolic, cardiovascular, and immune systems. Glucocorticoid enhances effective learning through the hippocampus and other normal body functions. That is why we remember events (either positive or negative) associated with strong emotions. Long-term secretion of GCs has catabolic effects. Thus, affecting animal health. Measuring GC is one of the ways of assessing animal welfare. But, high GC concentration does not only indicate pain or suffering. We report that stress and emotion trigger similar physiological responses. So, measuring GC levels cannot differentiate between positive and negative states. We conclude that GC shows circadian rhythms and episodic spikes in some species. Values from a single sample point are not reliable to make conclusions about a condition. Training animals for blood collection may reduce stress. Thus not causing bias in the GC concentration measured.

Selection signatures in melanocortin-1 receptor gene of turkeys (Meleagris gallopavo) raised in hot humid tropics.

Feather colours are used by avian species for defense, adaptation and signaling. Melanocortin-1 receptor (MC1R) gene is one of the genes responsible for feather colour. This study identified selection signatures in MC1R gene of Nigerian indigenous turkeys (NIT) using British United turkeys (BUT) as control breed to investigate the evolutionary processes that have shaped NIT with various feather colours. Complete MC1R gene of 146 NIT (76 males and 70 females) and 32 BUT (18 males and 14 females) were sequenced. Transition/transversion and codon usage biases were predicted using MEGA v6 software. The selective force acting on the gene was predicted using HyPhy software. The FST values were estimated using Arlequin v3.5. The highest transition/transversion bias was predicted for white BUT (1.00) while the lowest was predicted for black NIT (0.50). Negative dN-dS values, indicative of purifying selection, were observed in MC1R gene of all the turkeys. The highest pairwise FST was observed between the MC1R gene of white BUT and black NIT while the least was observed between lavender NIT and white NIT. No recombination event was observed in black NIT and white BUT. The relative synonymous codon usage was the same among different colours for some codons. Presence of purifying selection in MC1R gene of all the turkeys with different feather colours confirms that the gene plays role in many biological processes such as feather colouration, behaviour, pain perception, immunity, growth and adaptation. The results also suggested that the genetic mechanisms generating different feather colours in turkeys are conserved.

Corrigendum to "Which factor is more important for the welfare of broiler chickens: Intensity or duration of episodic heat stress?" [J. Therm. Biol. 99 (2021) 102981].

Due to the effects of global warming, there is a predicted increase in the frequency, intensity and duration of heat waves in the future. Little is known of how this could affect the welfare of broiler chickens. Sixty-four broiler chickens were subjected to either high heat stress (HHS; 32oC, 70% RH for 3 h), moderate heat stress (MHS; 30oC, 70% RH for 6 h), or normal conditions (NC: 20oC, 50% RH for 6 h) for two consecutive days. Half the birds had been subjected to anaesthesia and fitted with a body temperature-ID chip placed in the breast muscle. Core body temperature (CBT) was taken during pre-heat stress (PrHS), at the end of 3 h (3HS) and 6 h (6HS) of heat stress using a pocket reader and used to estimate change in CBT (ΔCBT). Surface body temperatures (SBTs) from under the wing (WT), feet (FT), cloaca (CLT) and comb (CT) were also measured, along with blood parameters, feed intake, daily weight gain and mortality. Data were analysed using General Linear Model and simple linear regression. At 3HS, CBT/ΔCBT and all SBTs showed this trend: HHS > MHS > NC (P<0.001). Blood pH, pCO2, iCa, HCO3- and TCO2 showed the same trend: HHS, MHS > NC (P<0.05). Comparing HHS for 3 h with MHS and NC for 6 h showed that CBT/ΔCBT, WT and CLT in HHS, MHS > control (P<0.001) while FT and CT showed a different trend (HHS > MHS > NC, P<0.001). Exposure of broiler chickens to 3 hours of HHS had dramatic effects on core and surface body temperatures. The effects of MHS were initially more modest yet, after a further 3 hours exposure, resulted in an increase in CBT which was close to that which HHS birds experienced after just 3 hours. This illustrates that duration of exposure to heat stress can have a critical effect, achieving similar life-threatening changes in body temperature that were observed under higher levels of heat stress but for half the time.

Environmental stress and livestock productivity in hot-humid tropics: Alleviation and future perspectives.

Tropical environments are characterized by persistently high temperature and relative humidity and the harsh environmental conditions pose a serious limitation on the optimal performance of the animals raised in this region. Heat stress causes deleterious effects on welfare, immunology and physiology of farm animals with a resultant impact on their productivity as the use of body resources is re-organized and the metabolic priorities of animals shift away from production, growth, health and reproduction. It is imperative to understand the mechanisms involved in the thermoregulation of animals under tropical conditions in order to develop appropriate strategies for their improvement. This review focuses on the available data on the increasing global temperature and the adverse impact of tropical conditions on animals' adaptive mechanism affected during thermal stress on production performance, intestinal and ileal microbiome, physiological responses, antioxidant system, metabolic responses, cellular and molecular response, adaptive mechanism strategies to heat stress and also strategies to palliate environmental stress on livestock under humid tropical conditions including environmental manipulation, genetic opportunity, epigenetic and feeding modification. Overall, the present review has identified the disturbance in the physiological indices of tropical livestock and the need for concerted efforts in ameliorating the adverse impacts of high ambient temperature aggravated by high humidity on livestock in tropical environments. Further research is needed on genotype-by-environment interaction on the thermotolerance of different livestock species in the tropics.

Which factor is more important: Intensity or duration of episodic heat stress on broiler chickens?

With the current global warming, there is a predicted increase in frequency, intensity and duration of heat waves in future. Little is known of how this could affect the welfare of broiler chickens. Sixty-four broiler chickens were subjected to either high heat stress (HHS; 32 °C, 70% RH for 3 h), moderate heat stress (MHS; 30 °C, 70% RH for 6 h), or normal conditions (NC: 20 °C, 50% RH for 6 h) for two consecutive days. On both days, the temperature-ID chips on all chipped birds were scanned during pre-heat stress (PrHS), end of 3 h (3HS) and 6 h (6HS) of heat stress using a pocket reader. Half of the chip birds' CBT was measured at the end of each hour of heat stress (HS: 1st -3rd hour). Surface body temperatures (SBTs) from under the wing (WT), feet (FT), cloaca (CLT) and comb (CT) were measured. Blood samples, feed intake, daily weight gain and mortality was also monitored. Data was analysed using General Linear Model and simple linear regression. At 3HS, CBT/ΔCBT and all SBTs showed this trend: HHS>MHS>NC (P<0.001). The regression equations to predict ΔCBT in HHS and MHS are ΔCBT = 0.917 + 0.663 h, P<0.05 and ΔCBT = 0.371 + 0.338 h, P<0.05 respectively. Blood pH, pCO2, iCa, HCO3- and TCO2 showed same trend: HHS, MHS > NC (P<0.05). Comparing HHS for 3 h with MHS and NC for 6 h shows that CBT/ΔCBT, WT and CLT in HHS, MHS>control (P<0.001) while FT and CT showed a different trend (HHS > MHS > NC, P<0.001). pCO2, feed intake and daily weight gain showed same trend (HHS, MHS > control). Temperature-ID chip (a less invasive technique) gave CBT/ΔCBT values that corresponded with the degree of heat stress experienced by the birds. Broilers were more tolerant to MHS than HHS after 3 h but MHS for 6 h and HHS for 3 h had similar impact.

Discriminant analysis of response to Newcastle disease and heat tolerance among chicken genotypes in hot humid tropical environment.

Newcastle disease and heat stress reduce the productivity of local chickens of Nigeria (LCN). This study compared the antibody response to Newcastle disease and heat tolerance among different LCN genotypes in hot humid tropics using multivariate discriminant analysis. A total of 299 birds were used for the study. Geometric mean titre against Newcastle disease before vaccination (GMTB), geometric mean titre against Newcastle disease after vaccination (GMTA), rectal temperature at week 4 (RT4), pulse rate at week 4 (PR4), respiratory rate at week 4 (RR4), heat stress index at week 4 (H4), rectal temperature at week 13 (RT13), pulse rate at week 13 (PR13), respiratory rate at week 13 (RR13) and heat stress index at week 13 (H13) were measured. All the traits were significantly (p < 0.05) affected by the genotype while sex differences were only observed in GMTB, GMTA and RR13. The stepwise discriminant analysis revealed RR4, PR13, RT13, H4, GMTA, GMTB, H13 and RT4 to be effective in differentiating the three chicken genotypes. Two canonical variables that accounted for 60.21% and 39.79% of the total variation were revealed. Linear discriminant functions for differentiation of the three chicken genotypes were also developed. 87.39% of normal feather, 76.58% of naked neck and 100% of frizzle feather chickens were correctly assigned into their genotypes. The longest Mahalanobis distance was observed between normal feather and frizzle feather chickens. The discriminant functions developed in this study could be used to differentiate the three genotypes of LCN using antibody response to Newcastle disease and heat tolerance.

In ovo injection of black cumin (Nigella sativa) extract on hatching and post hatch performance of thermally challenged broiler chickens during incubation.

The objective of this study was to investigate the effects in ovo injection of black cumin (BC) extract on chick's quality and response of thermally challenged broiler chickens. A total of 700 hatching eggs of broiler chickens (Marshall) were assigned to 7 treatments of 100 eggs each and incubated using the conventional protocol (37.8°C) for the first 10 d and then exposed to a high temperature (39.6°C) for 6 h daily from day 10 until day 18 of the incubation. At embryonic day 17.5, the eggs were randomly allotted to 7 treatment groups, viz.: eggs without in ovo injection (WA), eggs injected with 0.9% saline solution (SA), 3 mg ascorbic acid (AA), 2 mg BC (TB), 4 mg BC (FB), 6 mg BC (SB), and 8 mg BC (EB) extracts. Experiment was laid out in a Completely Randomized Design. After hatching, the chicks were reared separately according to in ovo treatments for 8 wk. Data were collected on hatchability, chick quality, internal organs, growth performance, plasma superoxide dismutase, malondialdehyde, and triiodothyronine (T3). The results showed that the hatchability of the eggs in the AA group was similar to that of SB eggs and higher than that of the other treatment groups. The intestinal weights of SB and EB birds were significantly higher (P < 0.05) than those of TB, SA, and WA. The final weights of the birds of SB and AA were higher (P < 0.05) than those of other treatments. The feed conversion ratio of the birds of TB and FB was comparable to that of EB and WA but higher than that of SB and AA. At hatch, the creatinine of the birds in SA and WA was similar to that of EB, FB, and TB but higher (P < 0.05) than that of AA and SB. Also, the plasma malondialdehyde, T3, and superoxide dismutase of SB and AA birds were better (P < 0.05) than those of the control groups. Overall, it was concluded that 6 mg of BC extract improved the antioxidant status and posthatch performance of thermally challenged broiler chickens.

Age- and sex-dependent regression models for predicting the live weight of West African Dwarf goat from body measurements.

The relationships between live weight and eight body measurements of West African Dwarf (WAD) goats were studied using 211 animals under farm condition. The animals were categorized based on age and sex. Data obtained on height at withers (HW), heart girth (HG), body length (BL), head length (HL), and length of hindquarter (LHQ) were fitted into simple linear, allometric, and multiple-regression models to predict live weight from the body measurements according to age group and sex. Results showed that live weight, HG, BL, LHQ, HL, and HW increased with the age of the animals. In multiple-regression model, HG and HL best fit the model for goat kids; HG, HW, and HL for goat aged 13-24 months; while HG, LHQ, HW, and HL best fit the model for goats aged 25-36 months. Coefficients of determination (R(2)) values for linear and allometric models for predicting the live weight of WAD goat increased with age in all the body measurements, with HG being the most satisfactory single measurement in predicting the live weight of WAD goat. Sex had significant influence on the model with R(2) values consistently higher in females except the models for LHQ and HW.