Nigerian indigenous hens show more discomfort-related behavior with visual separation than physical separation from their chicks: An exploratory study.

The Nigerian indigenous hens exhibit their full natural behavior repertoires, including maternal care. The strong maternal bond between the hen and her chicks is established prior to hatching. Maternal care of chickens is essential for both exotic and indigenous chickens. This study compared the behaviors of six hen-chick pairs in a physical (PHY) and visual (VIS) separation test for 10 min. All the six hen-chick pairs were subjected to PHY separation on the 8th day of post-hatch and a VIS separation on the 12th day of post-hatch. The PHY separation involved the use of a wire mesh to separate the hen from her chicks, while the VIS separation involved the use of a trampoline to separate the hen from her chicks. The hen's behavior was recorded during the 10-min separation period. Behaviors recorded included sitting, body shaking, pecking, movements toward the chicks, jumping, pacing, defecation, movements away from the chicks, and preening. We further grouped these nine behaviors into two categories: discomfort-related (pacing, movement toward chicks, body shaking, defecation, and jumping) and comfort-related (sitting, pecking, preening, and movement away from the chicks) behaviors. Before and after each separation, the hens were gently restrained, and a drop of blood was sampled from the wing vein to determine the blood glucose level. Their heart rate and eye temperature were also measured. A two-related samples test (Wilcoxon) was used to compare the behavior of the hens when subjected to the PHY and VIS separation. Eight out of the nine behaviors monitored did not differ between the separation types. However, the frequency of pacing by the hens was greater (z = -2.201, P = 0.028) in the VIS separation than in the PHY separation. Also, discomfort-related behavior was greater (t (5) = -2.717, P = 0.042) during the VIS separation than the PHY separation. Comfort-related behavior did not differ between the separation types. The change in eye temperature, heart rate, and blood glucose was similar in the two separation types. In conclusion, Nigerian indigenous hens displayed more discomfort-related behavior to the VIS separation from their chicks, but this was not associated with physiological responses indicative of stress.

An exploratory study on differences in maternal care between two ecotypes of Nigerian indigenous chicken hens.

The Yoruba (YRE) and Fulani (FLE) are the two notable indigenous chicken ecotypes in Nigeria. They exhibit broodiness and post-hatch care of their chicks. Studies on welfare, productivity, and maternal behaviors of these two ecotypes are scarce, hence the need for this study. Separate flocks of these ecotypes were housed intensively and hens that showed broodiness (ten YRE and five FLE) were monitored. Brooding behaviors were monitored for 3 days in the 1st and 2nd weeks of brooding and daily in the 3rd week of brooding for 6 h/day (07:00-09:00 h, 11:00-13:00 h, and 15:00-17:00 h). During brooding, surface body temperatures (eye, brood patch and under the wings), egg temperature and body weight of the hens were measured. Chicks hatched (44 chicks from the YRE and 24 chicks from the FLE) by these hens were subjected to tonic immobility tests on the 7th, 14th, and 21st days post-hatch and to a simulated predator test on the 8th, 15th, and 22nd days post-hatch to determine their level of fear. In each ecotype, brooding behaviors did not change over the three weeks, but the YRE hens spent longer time sitting on their eggs at the 2nd (U = 5.000, z = -2.454, P = 0.014) and 3rd (U = 9.000, z = -1.961, P = 0.050) week of brooding. The surface body temperatures of both ecotypes, egg temperature, and relative weekly weight loss were similar over the brooding period, but relative weekly weight loss was greater (P < 0.05) at the 3rd than 1st and 2nd week of brooding. The surface body temperatures were positively correlated (P < 0.01) with egg temperature. In both ecotypes, attempts to induce and duration of tonic immobility were similar over the test periods but on the 7th day post-hatch, the duration of tonic immobility was longer (U = 323.000, z = -2.632, P = 0.008) and on the 14th day post-hatch, the number of attempts to induce tonic immobility was less (U = 332.000, z = -2.630, P = 0.009) in the YRE chicks. In conclusion, YRE hens sat more on the eggs and their chicks were more fearful.

Effect of acute heat stress on cognitive performance of chickens in a feed-related discriminant task.

Little is known about immediate and long-lasting effect of acute heat stress on chicken cognition. Thirty-five, 9-week-old birds were trained to differentiate two cone colours; white (rewarded, R; with feed underneath) and black (unrewarded, UR; empty). The sixteen birds that learnt the task were randomly assigned to three temperature regimens (TR: 22-24 °C (control), 30-32 and 36-38 °C for 3h/day) for three consecutive days during which rectal (RT), wing (WT) and eye (ET) temperatures were monitored. After the 3 h of exposure, birds were allowed to rest for 1 h before the commencement of the discriminant task. The latencies to open the cones (R and UR) and proportion of cones opened were recorded. A long-lasting effect was tested a week after exposure to TR. TR had a significant effect on RT, WT and ET. The motivation to turn over R cones was weaker in birds exposed to 36-38 °C than birds exposed to 22-24 °C. Also, the proportion of R cones opened were fewer in birds that experienced TR of 36-38 °C compared to birds exposed to 22-24 °C and 30-32 °C specifically on two out of the three cognitive test days (Days 1 and 3). Latency and proportion of UR cones opened was not affected by TR. RT, WT and ET were all negatively and significantly correlated with latency to open the UR cones. Previous exposure of birds to three TR had no effect on the latency to open both cones but the proportion of R cones opened was greater in birds exposed to 30-32 °C compared to the 22-24 °C birds. In conclusion, an immediate (36-38 °C) and long-lasting effect (30-32 °C) of acute heat stress was associated with a weak motivation to perform feed related discrimination task.

Provision of Additional Cup Drinkers Mildly Alleviated Moderate Heat Stress Conditions in Broiler Chickens.

Sixty-four broilers of a commercial strain in a 2 × 2 factorial design with two environmental conditions: normal (N; 20°C, 50% RH) or MHS (30°C, 70% RH for 6 h/day for 3 consecutive days), and two drinker treatments; standard (B; a bell drinker/pen) or additional (A; as B + two cup drinkers). Each treatment had 16 birds with half of them implanted with temperature-ID chips to estimate the change in core body temperature (ΔCBT). Comb (CT) and feet (FT) temperatures were measured with atemperature probe, behavior was recorded with digital cameras, daily weight gain, feed intakes and litter moisture content (LMC) were also monitored. Data were subjected to General Linear Model (GLM) analysis. MHS broilers had greater ΔCBT, CT, and FT and more time spent panting and drinking compared to N broilers. Provision of additional cup drinkers reduced ΔCBT but enhanced heat loss from the comb and feet. At 6HS, MHS birds showed a tendency to spend more time drinking from the cup drinkers compared to N birds without compromising LMC. MHS suppressed feed intake and not weight gain.

Comparative study of adaptation in three chicken genotypes under humid tropical conditions of Nigeria.

Adaptation of Nigerian indigenous chickens (NIC), FUNAAB-α (F-α) and Transylvanian naked neck chickens (TNN) raised during hot-dry season under humid tropical environment was compared during a 4-week period. A well-adapted NIC (n = 12; 6 males and 6 females), a newly developed F-α (n = 12; 6 male and 6 females) and a newly introduced TNN (n = 12; 6 male and 6 females) chickens aged 36 weeks were used for this experiment. Heart rate (HR), respiratory rate (RR), rectal temperature (RT) and skin temperature on comb (COM), wattle (WAT), breast (BRE) and under wings (WIN) were monitored between 13.00 and 14.00h twice a week for four weeks. Wattle temperature (p = .017) and BRE (p = .000) differ significantly among the genotypes. Transylvanian naked neck chickens recorded higher WAT than NIC, though not different from F-α. Nigerian indigenous chickens had lower BRE temperature than other two genotypes. Significantly higher WAT (p < .001) and WIN (p < .05) were recorded in cocks than in laying hens. In contrast, laying hens had significantly (p < .001) higher RR and HSI than cocks. There was interactive (p < .05) effect of sex and genotype on BRE. Nigerian indigenous cocks and hens exhibited lower BRE than others, except F-α cock. There were positive significant correlations between WIN and WAT in the three genotypes. In NIC, COM had positive correlation with WAT (r = .532) and WIN (r = .696). Heart rate was positively correlated with WAT (r = .608) and WIN (r = .697) in F-α, and WIN (r = .512) in TNN chickens. In conclusion, the three genotypes can adequately adapt to the prevailing conditions of humid tropics. Reduction of feather on the neck and breast and higher heat loss capacity in TNN chickens could help the birds adapt to hot-humid environment. The two sexes could adapt to the tropical region. Cocks lose body heat predominantly via non-evaporative means while hens employ evaporative heat loss mechanism.

Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender.

Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk (TNM) extender was examined in this study. Pooled semen samples obtained from West African Dwarf (WAD) goat bucks were diluted with Tris-based extenders containing different levels of TNM (0, 5, 10, 15 and 20 ml/100 ml extender). The diluted semen samples were subjected to slow and rapid freezing for a period of 7 days and thereafter evaluated for sperm functional attributes (percentage motility, acrosome integrity, membrane integrity, abnormality and livability) and oxidative stress (malondialdehyde [MDA] concentration and acrosin activity) parameters. Results showed that higher (p < 0.05) motility, livability, membrane and acrosome integrities in semen cryopreserved with slow freezing compared to rapid freezing. These parameters (motility, livability and membrane integrity) were higher (p < 0.05) in semen cryopreserved with 15% TNM in both slow and rapid freezing protocols. The results revealed that semen cryopreserved in slow freezing had lower (p < 0.05) abnormality compared to rapid freezing. Acrosin activity was higher in slow freezing compared to rapid freezing. Acrosin activity was higher at 15% TNM in both slow and rapid freezing. Lower (p < 0.05) MDA concentration was observed in semen cryopreserved using slow freezing compared to rapid freezing. The findings revealed improved post-thaw sperm functional attributes and oxidative stress parameters of WAD goat spermatozoa cryopreserved with 15% TNM using slow freezing.

Elevated levels of the stress hormone, corticosterone, cause 'pessimistic' judgment bias in broiler chickens.

Pessimistic judgment biases, whereby humans or non-human animals interpret ambiguous information negatively, are hypothesised to be one of the suite of adaptive changes that comprise the vertebrate stress response. To test this hypothesis, we asked whether experimentally elevating levels of the glucocorticoid stress hormone, corticosterone, in broiler chickens produced a pessimistic judgment bias. We trained young chickens to discriminate a stimulus (paper cone) placed at two locations in an arena, one associated with reward (mealworms) and one with punishment (air puff). During seven days of non-invasive administration of either corticosterone or vehicle control, we tested the birds' responses to the cone placed at ambiguous locations between the trained locations. Corticosterone-treated birds were more likely than controls to respond as if punishment was likely when the cone was placed near to the punished location. The degree of this 'pessimism' was associated with smaller relative spleen weight, which is a documented consequence of chronic stress in chickens. We conclude that changes in corticosterone levels in chickens are sufficient to cause a specific change in decision making, dubbed 'pessimism', whereby corticosterone-treated birds showed an increased expectation of punishment in the face of ambiguous information. Pessimism could be a useful welfare indicator in chickens.