[Study of viral contamination of Japanese quail and cell cultures from their embryos]. / Izuchenie virusnoi kontaminatsii iaponskikh perepelov i kul'tury kletok iz ikh émbrionov.

Agents identified as adenovirus CELO were isolated from organ suspensions of 1500 Japanese quails (JQ) in 4 experiments. No contaminating viruses were found in examinations of cell cultures prepared from the kidneys of 80 JQ. Negative results were obtained in examinations of 163 antigens from JQ organs in the COFAL test. Agents identified as mycoplasma were isolated in 16 cases from 736 specimens of the virus-containing fluid used for manufacture of measles virus. According to the results of the CFT, 59 antigens prepared from Japanese quail embryo cell cultures contained no oncornavirus antigens and were negative in the COFAL test. Among 1848 JQ sera examined for the presence of antibody to leukemia-sarcoma complex viruses only 2 sera were found in 1976 to contain antibody to Rous sarcoma virus. No antibody to Marek disease virus in 414 serum samples or to Newcastle disease virus in 554 sera were detected. Among 500 sera tested 30.8% contained antibody to adenovirus CELO.

[Isolation and study of several properties of mycoplasma from Japanese quail embryos]. / Vydelenie i izuchenie nekotorykh svoistv mikoplazmy iz embrionov iaponskogo perepela.

An agent isolated from Japanese quail embryos was found to be pathogenic for chick embryo and quail embryo fibroblast cultures, to cause hemadsorption and hemagglutination of guinea pig erythrocytes. Reproduction of the agent was inhibited by tetracycline and 5-bromodeoxyuridine. The agent incorporated both 3H-thymidine and 3H-uridine and had a buoyant density in sucrose gradient of 1.22 g/ml. Mature mycoplasma forms were determined electron microscopically in gradient fractions in cell sections.