Subject(s)
N-Methylaspartate/pharmacology , Phospholipids/pharmacology , Polyamines/pharmacology , Spinal Cord/drug effects , Animals , Cells, Cultured , Dose-Response Relationship, Drug , Insulin/pharmacology , Ion Channel Gating/drug effects , Membrane Potentials , Neurons/drug effects , Neurons/physiology , Rats , Receptors, N-Methyl-D-Aspartate/physiology , Spinal Cord/physiologyABSTRACT
We compare the power spectra of activities of neural cultures from the cortex and spinal cord of embryonic rat. The measurements were made in a microelectrode culture chamber by non-invasive methods. The data suggest that the fundamental frequencies of cortical and spinal cultures significantly differ, while the general patterns of the spectra are rather similar.
Subject(s)
Cerebral Cortex/physiology , Neurons/physiology , Spinal Cord/physiology , Action Potentials/physiology , Animals , Cells, Cultured , Cerebral Cortex/cytology , Evoked Potentials/physiology , Fourier Analysis , Microelectrodes , Rats , Spinal Cord/cytologyABSTRACT
A multi-microelectrode culture chamber system was constructed for monitoring simultaneously morphological and electrophysiological development of neural cells in vitro. The setup consisted of a pattern of gold conductor lines evaporated onto a glass substrate and insulated with polyamide. The width of each electrode was 10 microns, and the distance between the electrodes was 60 microns. The electrode patterns were constructed and the uncovering of the electrode tips were carried out by photo-etching. This system allowed us to record spontaneous activities in both explant- and primary monolayer cultures of either rat or mouse spinal cords and forebrains, during neuronal regeneration and maturation.
