Altered States of Consciousness during Exercise, Active-Alert Hypnosis, and Everyday Waking State.

This retrospective study was a nonrandomized comparison of exercisers' (runners and participants of a spinning class) states of consciousness with subjects of active-alert hypnosis (AAH) and students in a class (control). Three hundred and seventy-five participants completed the Phenomenology of Consciousness Inventory (PCI). Runners, spinners, and participants of AAH scored higher on the altered experience and altered state of awareness dimensions of the PCI than the control group. Runners scored higher than participants of AAH and the control condition on the rationality dimension, and spinners scored higher than participants in the AAH condition. The AAH group scored lowest on the self-awareness dimension. On the volitional control dimension, the spinning and control groups scored significantly higher than the runner and AAH groups. The results suggest that exercise may lead to states of consciousness similar to AAH and thus increase responsiveness to a coach's training suggestions.

Phenomenological Experiences during Active-Alert Hypnosis: Comparison of Hypnotist and Subject.

There has been increasing clinical interest in active-alert hypnosis (AAH). However, relatively few studies have been devoted to studying its properties systematically. The present study compared the subjective experiences of subjects (31) and hypnotists (5) during AAH, using Pekala's Phenomenology of Consciousness Inventory (PCI), the Dyadic Interactional Harmony (DIH) scale and the Archaic Involvement Measure (AIM). Results demonstrated similarities between the experiences of subjects and hypnotists. The only significant difference between the subjects' and the hypnotists' experiences was shown by the PCI, which highlighted the differences stemming from the different roles of hypnotist and subject during the AAH. The study suggests it may be important to examine subjective descriptors in the exploration of personal experiences in studies of AAH.

RECALLED PARENTAL REARING STYLE AND DIMENSIONS OF HYPNOTIC RESPONSE.

So far, only a few studies have investigated how memories of parental rearing style are associated with hypnotic response, and these were either qualitative or confined to the behavioral aspect of hypnotizability. The present study aims to employ standardized, quantitative measures to investigate the associations between recalled parental rearing style and the behavioral, phenomenological, and emotional dimensions of hypnotic response. Two samples of healthy adult subjects (N = 438) completed a questionnaire on their parents' behavior and participated in a standard group hypnosis session in which their hypnotizability score, hypnotic experiences, and archaic involvement were assessed. Memories of cold and punishing parental behavior were associated with negative experiences related to the hypnotic state and negative emotions toward the hypnotist. The authors conclude that assessing parental behavior may be important in planning hypnotherapeutic interventions.

A Neutral Control Condition for Hypnosis Experiments: "Wiki" Text.

A new control condition called Wiki is introduced. Key themes of each test suggestion of the Stanford Hypnotic Susceptibility Scale, Form C, were matched by a corresponding extract from Wikipedia.org. The authors compared phenomenological reports of participants across 4 conditions: hypnosis split into high and low hypnotizable subgroups, music, and Wiki condition, using the Phenomenology of Consciousness Inventory. High hypnotizables undergoing hypnosis reported higher altered experience and altered states of awareness than individuals in the Wiki condition, supporting the authors' hypothesis that the Wiki condition does not evoke an altered state of consciousness (internal dialogue, volitional control, and self-awareness did not differ). Wiki might be a viable control condition in hypnosis research given further examination.

Investigation of a role for Glu-331 and Glu-305 in substrate binding of tripeptidyl-peptidase II.

The aim of this study was to investigate the mechanism by which tripeptidyl-peptidase II (TPP II) can specifically release tripeptides from the free N-terminus of an oligopeptide. The subtilisin-like N-terminal part of TPP II was modelled using subtilisin as template. Two glutamate residues (Glu-305 and Glu-331) appeared to be positioned so as to interact with the positively charged N-terminus of the substrate. In order to test this potential interaction, both residues were replaced by glutamine and lysine. The catalytic efficiency was reduced 400-fold for the E331Q variant and 20000-fold for the E331K variant, compared with the wild-type (wt). A substantial part of this reduction was due to decreased substrate affinity, since the K(M) for both mutants was at least two orders of magnitude greater than for the wt. This decrease was linked specifically to interaction with the free N-terminal amino group, based on inhibition studies. Glu-305 appears to be essential for enzymatic activity, but the extremely low activity of the E305Q variant prevented an investigation of the involvement of Glu-305 in substrate binding. The present work is, to our knowledge, the first report to investigate a mechanism for a tripeptidyl-peptidase activity through site-directed mutagenesis.

Identification of the catalytic triad in tripeptidyl-peptidase II through site-directed mutagenesis.

Tripeptidyl-peptidase II (TPP II) is a 138-kDa subtilisin-like serine peptidase forming high molecular mass oligomers of >1000 kDa. The enzyme participates in general protein turnover and apoptotic pathways, and also has specific substrates such as neuropeptides. Here we report the site-directed mutagenesis of amino acids predicted to be involved in catalysis. The amino acids forming the putative catalytic triad (Asp-44, His-264, Ser-449) as well as the conserved Asn-362, potentially stabilizing the transition state, were replaced by alanine and the mutated cDNAs were transfected into human embryonic kidney (HEK) 293 cells. In clones stably expressing the mutant proteins, TPP II activity did not exceed the endogenous activity, thus confirming the essential role of the above amino acids in catalysis. Mutant and wild-type TPP II subunits co-eluted from a gel filtration column, suggesting that the subunits associate and that the native subunit conformation was retained in the mutants. Interestingly, the S449A and a H264A mutant enzyme affected the quaternary structure of the endogenously expressed TPP II, resulting in formation of an active, larger complex of >10,000 kDa.