Outbreak of Oropouche virus in frontier regions in western Amazon.

Oropouche virus (OROV) is characterized as a re-emerging arbovirus of great concern for public health, being responsible for several outbreaks of acute fever identified in Latin American countries, registering more than half a million reported cases. The incidence of reports of this virus is intrinsically favored by environmental conditions, in which such characteristics are related to the increase and distribution of the vector population to areas of human traffic. Moreover, there is a problem regarding the lack of diagnosis in Brazil that aggregates the success of the etiologic agent. Thus, by means of molecular techniques, we identified 27 positive cases of the OROV circulating in border locations in western Amazon, with 44.44% (12/27) of the cohort characterized as infected individuals with reported symptoms, mainly ranging from fever, myalgia, and back pain. Among the positive samples, it was possible to obtain a total of 48.14% (13/27) samples to analyze the S and M segments of Oropouche, which showed similarities among the Brazilian sequences. Thus, it was possible to verify the circulation of the OROV in Rondonia and border areas, in which the tracking of neglected arboviruses is necessary for the genomic surveillance of emerging and re-emerging viruses.IMPORTANCEThe western Amazon region is known for outbreaks of acute febrile illnesses, to which the lack of specific diagnostics for different pathogens hinders the management of patients in healthcare units. The Oropouche virus has already been recorded in the region in the 1990s. However, this is the first study, after this record, to perform the detection of individuals with acute febrile illness using a screening test to exclude Zika, dengue, and chikungunya, confirmed by sequencing the circulation of the virus in the state of Rondonia and border areas. We emphasize the importance of including diagnostics for viruses such as Oropouche, which suffers underreporting for years and is related to seasonal periods in Western Amazon locations, a factor that has a direct influence on public health in the region. In addition, we emphasize the importance of genomic surveillance in the elucidation of outbreaks that affect the resident population of these locations.

The emergence of Omicron VOC and its rapid spread and persistence in the Western Amazon.

Genomic surveillance represents a strategy to understanding the evolutionary mechanisms, transmission, and infectivity of different SARS-CoV-2 variants. We evaluated 603 individuals positive for SARS-CoV-2 from 34 municipalities of Rondônia between December 2021 to December 2022. Nasopharyngeal samples were collected, RNA was extracted and screened using RT-qPCR for VOCs. RNA of the samples were sequenced and further analyzed for phylogeny, mutations, and lineages, totaling 96.19% of samples positive for Omicron VOC in this cohort. We observed that most individuals had at least two doses, however 18.97% were not vaccinated with any dose. 554 sequences were amenable to analysis for alignment and phylogenetic characterization; this group corresponded to the 27 subvariants of the Omicron VOC; a total of 100 mutations were identified, 48% of which were found in the S gene. In conclusion, the data demonstrated the rapid spread and persistence of Omicron VOC in Rondônia during the 12-month study period. Although high frequency of mutations was found in the analyzed samples, there were no individuals with a severe clinical profile, demonstrating that vaccination had a positive effect in those cases.

Early identification of Mexican American children who are at risk for becoming obese.

OBJECTIVE: To describe the problem of obesity in Mexican American children and provide a method for identifying Mexican American children at risk for obesity. DESIGN: Cross-sectional and retrospective cohort study. SUBJECTS: Mexican American children, ages 5-11 (kindergarten (K)-5th grade) were studied in cross-sectional (girls = 463, boys = 448) and retrospective (girls = 124, boys = 92) samples. MEASUREMENTS: Weights (kg) and heights (cm) were extracted from each child's school medical records. Measurements were made by the school nurses (one nurse per school). Body mass index (BMI [w/h2]) was calculated from obtained weights and heights. RESULTS: The prevalence of obesity in the cross-sectional sample was 27.4% for girls and 23.0% for boys. The highest prevalence was noted in 4th grade for both the girls (32.4%) and boys (43.4%). The 5 year incidence rates derived from the retrospective sample were 17.7% for girls and 21.1% for boys. The largest increase in the number of girls becoming obese was observed between K and 1st grade (15.6%). The largest increase for boys was found between the 1st and 2nd grades (13.2%). Logistic regression parameters were calculated to estimate the probability of becoming obese in 5th grade given a child's K BMI. Using the generated equation, BMIs in K equal to 16.5, 20.9, and 23.7 would have a 21.0%, 70.0%, and 91.0% probability of being obese by 5th grade. CONCLUSION: This study provides schools with a simple procedure for identifying Mexican American children at risk for obesity. This procedure may assist in the development and implementation of interventions aimed at averting the onset of obesity in this population.