1.
J Cell Physiol
; 110(1): 101-4, 1982 Jan.
Article
in English
| MEDLINE
| ID: mdl-7068764
ABSTRACT
WEHI-3 cell-conditioned medium with the capacity to stimulate megakaryocyte colony formation was separated by Sephadex G-150 column chromatography. The development of colonies containing megakaryocytes was observed only when mixing experiments were performed. Individual fractions did not support megakaryocyte colony growth. The two factors in WEHI-3 CM required for megakaryocyte colony growth had apparent average molecular weights of 35,000 daltons (megakaryocyte CSF) and 100,000 daltons (megakaryocyte potentiator). The results were confirmed in serum-free conditions in which colonies were directly identified in the cultures by acetylcholinesterase staining. Two growth factors may be necessary for the genesis of megakaryocytic colonies.
Subject(s)
Growth Substances/physiology , Leukemia, Experimental/physiopathology , Megakaryocytes/physiology , Animals , Bone Marrow/physiology , Cell Line , Culture Media , Growth Substances/isolation & purification , Kinetics , Mice , Mice, Inbred C57BL
2.
Primary epithelial cell culture of adult rat kidney, enhancement of cell growth by ammonium acetate.
Exp Cell Res
; 121(1): 47-54, 1979 Jun.
Article
in English
| MEDLINE
| ID: mdl-36281
Subject(s)
Cell Division/drug effects , Quaternary Ammonium Compounds/pharmacology , Acetates/pharmacology , Ammonium Chloride/pharmacology , Animals , Cell Survival/drug effects , Cells, Cultured , Cytoplasmic Granules/drug effects , Epithelial Cells , Kidney/cytology , Male , Nucleotidases/metabolism , Rats , gamma-Glutamyltransferase/metabolism
3.
JAMA
; 234(12): 1221, 1975 Dec 22.
Article
in English
| MEDLINE
| ID: mdl-1242739
4.
Br Vet J
; 127(10): 51-3 passim, 1971 Oct.
Article
in English
| MEDLINE
| ID: mdl-4943868
5.
J Anim Sci
; 33(4): 872-7, 1971 Oct.
Article
in English
| MEDLINE
| ID: mdl-5092780
Subject(s)
Basal Metabolism , Potassium/administration & dosage , Sheep/growth & development , Sodium/administration & dosage , Adipose Tissue/analysis , Analysis of Variance , Animal Nutritional Physiological Phenomena , Animals , Body Weight/drug effects , Fats/analysis , Feeding Behavior/drug effects , Food Additives , Muscles/analysis , Nutritional Requirements , Proteins/analysis
6.
Am Surg
; 34(6): 442-5, 1968 Jun.
Article
in English
| MEDLINE
| ID: mdl-5651498