Linking trauma to mental health in the statewide Texas Youth Depression and Suicide Research Network (TX-YDSRN).

Rates of youth depression and suicide are rising worldwide and represent public health crises. The present study examined the relationship between trauma history and symptoms of depression, suicidal ideation, and anxiety among suicidal and depressed youth. A diverse group of 1000 8-20-year-olds enrolled in the statewide Texas Youth Depression and Suicide Research Network (TX-YDSRN) reported their trauma history (Traumatic Events Screening Inventory for Children) and symptoms of depression (Patient Health Questionnaire for adolescents; PHQ-A), anxiety (Generalized Anxiety Disorder scale; GAD-7), and suicidality (Concise Health Risk Tracking scale; CHRT-SR). Nearly half of the sample reported exposure to multiple categories of traumatic experiences. Number of trauma exposure categories significantly predicted PHQ-A and GAD-7 scores. Exposure to interpersonal trauma and to sexual trauma were significantly associated with PHQ-A, GAD-7, and CHRT-SR scores. The number of trauma exposure categories was associated with increased levels of anxiety and depression; however, only exposure to interpersonal or sexual trauma was associated with more suicidality. Clinicians should assess trauma exposure in patients seeking psychiatric care, especially for interpersonal and sexual trauma, which may be predictive of increased risk for suicidality in depressed youth. Future work should disentangle the effects of specific trauma types from multiple trauma exposure.

Automated Immunoassay Performed on a 3D Microfluidic Paper-Based Device for Malaria Detection by Ambient Mass Spectrometry.

Microfluidic paper-based analytical devices (µPADs) are emerging as a prominent platform for disease detection, specifically in developing countries. This paper device offer simplicity and affordability not typically seen in centralized laboratory settings. However, detection limits in µPADs are inadequate and often require test results to be read within a specific time interval to ensure accuracy. To overcome these challenges, we are developing an on-chip mass spectrometry (MS) detection strategy for immunoassays performed on paper substrates. Herein, we present our initial results from a proof-of-concept study toward the development of µPADs capable of storing immunoassay reagents within the confinements of the 3D device, automatic splitting of biofluid into four individual test zones, immuno-capture of the disease biomarker, and on-chip MS detection of the captured species. The reported study encourages the development of point-of-care and direct-to-customer testing using disposable µPADs to collect samples, followed by sensitive analysis using portable MSs. We demonstrate this capability using malaria Plasmodium falciparum histidine-rich protein 2 (PfHRP2) antigen detection.

Enhanced thread spray mass spectrometry: a general method for direct pesticide analysis in various complex matrices.

Determination of pesticide residues in a wide variety of matrices is an ongoing challenge due to low concentration and substantial amounts of interfering endogenous compounds that can be coextracted with the analytes. Herein, we describe the use of cellulose thread both as a suitable sampling medium for various matrices and as a direct analysis platform through an improved thread spray mass spectrometry (MS) approach. Enhanced extraction and the subsequent generation of tiny nanodroplets, after the application of DC potential to the wet thread, enabled ultra-sensitive detection of pesticides without prior sample treatment. This methodology was applied to quantify glyphosate and its metabolite, aminomethylphosphonic acid, in surface water at 12.2 µg mL-1 limit of detection (LOD) via standard addition calibration. The method was also used for an internal standard calibration for the analysis of atrazine, which resulted in a LOD of 0.74 ng mL-1. The enhanced thread spray MS platform also proved effective when applied for direct analysis of diphenylamine and thiabendazole, which enabled the evaluation of post-harvest pesticide treatment of fruits (surface and interior) without complete destruction of the fruits.

Direct differentiation of whole blood for forensic serology analysis by thread spray mass spectrometry.

Direct analysis of whole blood on bloodstained textiles is achieved with thread spray mass spectrometry (MS). This capability satisfies investigators' first priority in crime scene investigations, which is determining if a stain is blood. This thread spray method explores the use of evidentiary fabric threads for rapid determination of hemoglobin directly from whole blood within textiles without prior extraction steps. The multiplicity of information that can be derived from the thread spray MS method distinguishes it from the current presumptive Bluestar® method, by enabling the detection of hemoglobin (both α- and ß-chains), the heme co-factor and lipids all from a single blood sample. Lipid composition was found to differ for blood samples originating from human, canine, and horse species. The robustness of the thread spray MS method as a forensic analytical platform was evaluated in three ways: (1) its successful applicability to samples previously tested by the Bluestar® presumptive method, offering a confirmatory test without prior sample pre-treatment, (2) successful detection of heme from previously washed fabrics, which demonstrated the unprecedented sensitivity of the thread spray method, and (3) the ability to analyze samples stored under ambient conditions for up to 30 days. These results attest to the potential capabilities of the thread spray MS platform in forensic serology, and its application for direct analysis of evidentiary garments, which confer the advantages of rapid analysis and the reduction of the false positive and negative identification rates for blood on textiles.

Microsampling with cotton thread: Storage and ultra-sensitive analysis by thread spray mass Spectrometry.

Storage and quantitative analysis of small volumes of biofluids are challenging, especially when low concentrations of analytes are to be detected in the presence of complex matrices. In this study, we describe an integrated thread-based approach for stabilizing small blood volumes in the dry-state at room temperature, while also offering direct analysis capabilities via thread spray mass spectrometry. The analytical merits of this novel microsampling platform was demonstrated via the direct analysis of diazepam and cocaine in dried blood samples stored for 42 days. In-situ in-capillary blood processing from hydrophobic threads enabled limits of detection as low as parts-per-quadrillion to be reached. We validated this ultra-sensitivity by analyzing small tissue-like residues collected after pushing a thread through the sample once. The implications of this sample collection, storage, and analysis platform can be extensive with direct applications in forensics and clinical studies.

Thread spray mass spectrometry for direct analysis of capsaicinoids in pepper products.

Thread spray ambient ionization uses single threads as a medium for sampling and ionization. This approach was demonstrated through the detection of various capsaicinoids from the interior of pepper fruits without destruction of the sample. Pepper residues present on the thread were analyzed by the application of DC voltage and solvent to cause field-induced charged droplet generation. Capsaicinoids extracted from the sample are contained in the electrosprayed droplets and transported to the mass spectrometer for characterization. The thread spray mass spectrometry method was optimized using commercially available materials like 100% cotton, cotton:polyester (35/65), 100% polyester, and nylon fabrics and subsequently applied for in-situ analysis of six different pepper fruits and pepper spray residues on fabrics. The results indicated that the special physico-chemical characteristics of threads allowed a rapid and convenient sampling and ionization of pepper products for analysis by mass spectrometry. The total capsaicinoid ion yields for the various pepper products correlated very well with that reported in Scoville Heat Units, suggesting that quantitative assessment of pungency levels may be achieved via the direct sample analysis without prior separation.