Development of curcumin-loaded nanoemulsion stabilized with texturized whey protein concentrate: Characterization, stability and in vitro digestibility.

The impacts of pH (2.8, 4.5, and 7.2) and extrusion cooking temperature (60°C, 85°C, and 110°C) on properties of native whey protein concentrate (NWPC) were evaluated, followed by delivering of curcumin through a nanoemulsion system stabilized with extruded WPC (EWPC). Protein solubility, surface hydrophobicity, and emulsion properties such as emulsion activity index (at 1% [w/w] protein concentration), stability index (at 0.5%, 1%, 2%, and 4% [w/w] protein concentration) and creaming index (evaluated at different protein concentrations [0.5%, 1%, 2%, and 4% w/w] and oil levels [20%, 40%, 60%, and 80%]) were improved as a function of the extrusion process. It was found that both covalent and non-covalent interactions contributed to the stabilization of the extrudates. The rheological investigation of the emulsions stabilized with EWPC (at different oil levels [20%, 40%, 60%, and 80%]) revealed high viscosity and shear thinning behavior as well as much higher G' and G″ values. Encapsulation efficiency was increased from 90.8% to 95.7% when NWPC and EWPC were used, respectively. The curcumin-loaded nanoemulsion containing EWPC presented high stability in confronting with ionic strength (NaCl salt with a concentration of 0.1-1 M), pH (3, 5, and 7), thermal treatments (pasteurization at 63°C for 30 min and sterilization at 95°C for 10 min) and storage time (1 month at 4°C and 25°C). In vitro release behavior revealed that samples stabilized with EWPC showed burst release in simulated intestine conditions. However, it was more stable in stomach conditions.

Impact of ultrasound treatment on the physicochemical and rheological properties of acid hydrolyzed sorghum starch.

The present study aimed to evaluate the influence of ultrasonication on the physicochemical properties of native and acid-hydrolyzed white sorghum starch. Sorghum starch exhibited improved freeze-thaw stability, solubility, swelling power, and paste clarity after mild sonication. Starches sonicated at 30 % amplitude for 10 and 20 min increased the peak viscosity to 249 and 240 BU, gel firmness to 140.23 and 131.62 (g), ΔH to 13.4 and 13.1 (J/g), crystallinity to 29.51 and 29.10 (%), double helix content to 1.11 and 1.07 and degree of ordered structures to 1.16 and 1.09. The sonicated dual-treated samples (sonicated-acid hydrolyzed) exhibited reduced swelling power, peak viscosity, gelatinization temperatures and gel firmness. In contrast, the solubility, paste clarity, ΔH, percentage of crystallinity, double helix content and degree of ordered structures improved. Ultrasonic treatment made cracks and holes in the granule surface, whereas dual-treated starches were more porous and rougher, with deep depressions. All sorghum starches displayed shear-thinning behavior (n < 1). The pseudoplastic behavior and consistency indices of the starch paste decreased with increasing sonication time and amplitude. The G' was always higher than G" and tanδ was <1 for all samples, indicating a more solid/elastic behavior. The increased sonication time and amplitude, as well as the dual-treatment, caused the gel to become more susceptible to shear forces, which resulted in a decrease in G' and G" and an increase in tanδ.

System network analysis of Rosmarinus officinalis transcriptome and metabolome-Key genes in biosynthesis of secondary metabolites.

Medicinal plants contain valuable compounds that have attracted worldwide interest for their use in the production of natural drugs. The presence of compounds such as rosmarinic acid, carnosic acid, and carnosol in Rosmarinus officinalis has made it a plant with unique therapeutic effects. The identification and regulation of the biosynthetic pathways and genes will enable the large-scale production of these compounds. Hence, we studied the correlation between the genes involved in biosynthesis of the secondary metabolites in R. officinalis using proteomics and metabolomics data by WGCNA. We identified three modules as having the highest potential for the metabolite engineering. Moreover, the hub genes highly connected to particular modules, TFs, PKs, and transporters were identified. The TFs of MYB, C3H, HB, and C2H2 were the most likely candidates associated with the target metabolic pathways. The results indicated that the hub genes including Copalyl diphosphate synthase (CDS), Phenylalanine ammonia lyase (PAL), Cineole synthase (CIN), Rosmarinic acid synthase (RAS), Tyrosine aminotransferase (TAT), Cinnamate 4-hydroxylase (C4H), and MYB58 are responsible for biosynthesis of important secondary metabolites. Thus, we confirmed these results using qRT-PCR after treating R. officinalis seedlings with methyl jasmonate. These candidate genes may be employed for genetic and metabolic engineering research to increase R. officinalis metabolite production.

Preparation and characterization of active Cirish fructans-fish gelatin film: Physicochemical, antioxidant, and antimicrobial properties.

This study was aimed to evaluate the film-forming ability and characterization of ultrasonically extracted Cirish fructans (CF) and CF-cold-water fish gelatin (G) composite films. The film-forming solutions were prepared at different levels (CF100-0G, CF75-G25, CF50-G50, CF25-G75, and CF0-G100) and the corresponding data were analyzed based on one-way analysis of variance. The results indicated that CF addition led to an impressive increase in composite films thickness (69.38-86.45 µm), moisture content (16.05%-27.8%), surface hydrophobicity, tensile strength (5.73-17.89 MPa), elongation at break (0.83%-1.66%), Young's modulus (77.12-88.15 MPa), and Tg (38.83-47.4°C) which CF75-G25 had the highest values. Meanwhile, the solubility (77.12%-88.15%), WVP (1.89-2.86 × 10-10gm-1 s-1 Pa-1), and oxygen permeability (1.53-3.26 × 1014 cm3 m-1 s-1 Pa-1) of the composite films decreased. The FTIR spectra indicated the protein-polysaccharide interactions and revealed that the secondary structure of gelatin was shifted from triple-helix structure (1661 cm-1) toward α-helix structure (1650-1657 cm-1) when CF was incorporated. The microstructure observations indicated that unlike gelatin film, CF film exhibited the smooth and uniform surface without cracks and phase separation. It was found that CF films had high total phenolic content (6.73 mg GEA g-1) and showed DPPH radical scavenging activity (67.86%). On the other hand, it showed inherent antimicrobial activity against both gram-positive and gram-negative bacteria. The results indicated that CF and CF-cold fish gelatin have great potential as active films with improved physical, mechanical, and barrier properties.

Accuracy of magnetic resonance imaging in assessing knee cartilage changes over time in patients with osteoarthritis: A systematic review.

Magnetic resonance imaging (MRI) is a technique useful for the diagnosis of cartilage damage due to high sensitivity to identify subchondral bone abnormalities and full-thickness cartilage lesions. The lack of a study on knee cartilage changes over time in patients with osteoarthritis (OA) by MRI technique led us to investigate the accuracy of MRI in identifying knee cartilage changes over time in patients with OA in a systematic review. In the present systematic review, started from the beginning of 2020 in one of the University Hospitals in Iran, the databases of CINAHL, Ovid, Elsevier, Scopus, PubMed, Science Direct, and Web of Science were searched using the keywords MRI, OA, Cartilage Lesion, Imaging Techniques. A total of 169 articles were retrieved in the initial search, and after reviewing the titles, abstracts, and full-texts, finally, seven were enrolled in the systematic review. Review of the selected papers showed that a total of 1091 subjects were studied, of which 355 were males. The results of all the studies, except one, indicated the high accuracy of MRI to identify knee cartilage changes over time. MRI technique can show cartilage changes with high accuracy in patients with knee OA over time. We proved the potential of MRI to identify articular cartilage injuries in patients with OA and its importance to the evaluation of articular cartilage lesions along with other available techniques.

Defining the Best Nasal Tip Projection among Iranian Women.

Rhinoplasty is one of the most complicated aesthetic surgeries. One important factor in nasal profile analysis before surgery is the NTP (Nasal Tip Projection). There has been controversy over defining the best tip projection and due to cultural differences there is a need to find the best formulation for Iranian noses. We selected 50 randomized patients. Lateral nasal views were captured from all of the patients. In order to equalize the photos, all tip rotations changed first to 105. We selected four methods for measuring NTP (Goode, Crumley 1, Crumley 2, and Powell and Humphreys). Based on these methods NTP was shown in four pictures. A questionnaire was designed for rating the pictures. Questionnaires were filled in by 3 different groups: rhinoplasty surgeons, general people, and artists. A total of 73 questionnaires were filled in. The analysis and comparison were done. Crumley 2 is the best NTP measurement method from the surgeons' and artists' view. Goode is the method preferred by general people. Powell & Humphreys method seems to be the worst method from all 3 groups' view. It seems that general people prefer smaller noses, because projection in Goode method is almost less than Crumley 2.