Diosgenin, a steroidal saponin, exhibits anticancer activity by attenuating lipid peroxidation via enhancing antioxidant defense system during NMU-induced breast carcinoma.

Diosgenin, a natural steroidal saponin, has been reported to be found predominantly in fenugreek and has diverse biological properties. N-Methyl-N-nitrosourea (NMU) is a mammary gland-specific carcinogen that closely mimics human breast cancer in many aspects. The aim of this study was to investigate the anticarcinogenic property of diosgenin with reference to lipid peroxidation, status of antioxidants, and activities of marker enzymes against NMU-induced experimental mammary carcinogenesis. Breast cancer was induced in female Sprague Dawley rats by an intraperitoneal administration of a single dose of NMU (a concentration of 50 mg/kg body weight) diluted in 0.9% saline, and the rats were treated with oral diosgenin, 20 mg/kg body weight, for 45 days. The results were interesting, and the diosgenin treatment remarkably downregulated the peroxidation reaction and marker enzymes and extraordinarily enhanced the indigenous antioxidant defense system. The factor for this remarkable restoration might be due to the effect of the intervention strategy on the downregulation of the peroxidation reaction through the strong antioxidant nature, which ultimately reflected in the downregulation of marker enzyme activities. The histopathological study of breast and liver tissues inevitably confirms the biochemical changes. Thus, it can be concluded that diosgenin exhibits anticarcinogenic activity via reducing peroxidation reaction and marker enzymes through enhancing the intrinsic antioxidant defense system.

Bifid vermiform appendix: a case report.

INTRODUCTION: Appendiceal anomalies are extremely rare: they have a reported incidence of between 0.004% and 0.009% of appendectomy specimens. The authors report a case of a patient who was found to have 2 appendices at emergency laparotomy, review the classification system used, and discuss the potential clinical pitfalls of similar cases. CASE REPORT: A 23-year-old man was admitted as an emergency with abdominal pain and vomiting. The operative finding was of a bifid appendix. One appendix was grossly gangrenous and lacked a mesoappendix, whereas the other had a mesoappendix and appeared macroscopically normal. The appendices shared a common base that arose from the cecum in the typical anatomical position. No other intra-abdominal malformations were present at inspection during laparotomy. Histology confirmed features of gangrenous appendicitis. His postoperative recovery was uneventful, and he was discharged after 4 days. CONCLUSION: Although rare, it is important for several reasons that surgeons are aware of the potential anatomical anomalies and malpositions of the vermiform appendix: first, a missed second appendix may result in serious clinical and medico-legal consequences; second, a double appendix can be confused with other intra-abdominal conditions; and finally, they can be associated with other congenital abnormalities.