ABSTRACT
The COVID-19 pandemic has become the leading societal concern. The pandemic has shown that the public health concern is not only a medical problem, but also affects society as a whole; so, it has also become the leading scientific concern. We discuss in this treatise the importance of bringing the world's scientists together to find effective solutions for controlling the pandemic. By applying novel research frameworks, interdisciplinary collaboration promises to manage the pandemic's consequences and prevent recurrences of similar pandemics.
Subject(s)
Biomedical Research/organization & administration , Coronavirus Infections/epidemiology , Delivery of Health Care, Integrated/organization & administration , Emergencies , Health Services Needs and Demand , Pandemics , Pneumonia, Viral/epidemiology , Betacoronavirus/pathogenicity , Biomedical Research/methods , COVID-19 , Coronavirus Infections/therapy , Coronavirus Infections/virology , Delivery of Health Care, Integrated/methods , History, 21st Century , Humans , Interdisciplinary Communication , Interdisciplinary Studies , Pneumonia, Viral/therapy , Pneumonia, Viral/virology , Public Health/history , Public Health/standards , SARS-CoV-2ABSTRACT
Currently, there is a great deal of interest in the study of natural compounds with free-radical-scavenging activity because of their potential role in maintaining human health and preventing diseases. In this paper, we report the antioxidant and cytoprotective properties of 2,4,8-trihydroxybicyclo [3.2.1]octan-3-one (TBO) isolated from the aqueous extract of Decalepis hamiltonii roots. Our results show that TBO is a potent scavenger of superoxide (O(2)·-), hydroxyl (·OH), nitric oxide (·NO) and lipid peroxide (LOO·) - physiologically relevant free radicals with IC(50) values in nmolar (42-281) range. TBO also exhibited concentration-dependent secondary antioxidant activities such as reducing power, metal-chelating activity and inhibition of protein carbonylation. Further, TBO at nmolar concentration prevented CuSO(4)-induced human LDL oxidation. Apart from the in vitro free-radical-scavenging activity, TBO demonstrated cytoprotective activity in primary hepatocytes and Ehrlich ascites tumour (EAT) cells against oxidative-stress-inducing xenobiotics. The mechanism of cytoprotective action involved maintaining the intracellular glutathione (GSH), scavenging of reactive oxygen species (ROS) and inhibiting lipid peroxidation (LPO). Based on the results, it is suggested that TBO is a novel bioactive molecule with implications in both prevention and amelioration of diseases involving oxidative stress as well as in the general well-being.
Subject(s)
Bridged Bicyclo Compounds/pharmacology , Free Radical Scavengers/pharmacology , Animals , Carcinoma, Ehrlich Tumor , Cell Line, Tumor/drug effects , Drug Evaluation, Preclinical , Hepatocytes/drug effects , Humans , Hydroxyl Radical/metabolism , Iron Chelating Agents/pharmacology , Lipid Peroxidation/drug effects , Lipoproteins, LDL/analysis , Liver/drug effects , Male , Medicine, Ayurvedic , Mice , Molecular Structure , Oxidants/pharmacology , Plant Roots/chemistry , Plants, Medicinal/chemistry , Protein Carbonylation/drug effects , Rats , Superoxides/metabolism , Xenobiotics/toxicityABSTRACT
The steam-distilled oil of cinnamon (Cinnamomum zeylanicum) flowers was analyzed by GC and GC-MS. It consists of 23% hydrocarbons and 74% oxygenated compounds. A total of 26 compounds constituting approximately 97% of the oil were characterized. (E)-Cinnamyl acetate (41.98%), trans-alpha-bergamotene (7.97%), and caryophyllene oxide (7.2%) are found to be major compounds. This is the first report on the chemical composition of the flower oil of Cinnamomum zeylanicum.
Subject(s)
Cinnamomum zeylanicum , Plant Oils/chemistryABSTRACT
Curcumin, the yellow color pigment of turmeric, is produced industrially from turmeric oleoresin. The mother liquor after isolation of curcumin from oleoresin contains approximately 40% oil. The oil was extracted from the mother liquor using hexane at 60 degrees C, and the hexane extract was separated into three fractions using silica gel column chromatography. These fractions were tested for antibacterial activity by pour plate method against Bacillus cereus, Bacillus coagulans, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Fraction II eluted with 5% ethyl acetate in hexane was found to be most active fraction. The turmeric oil, fraction I, and fraction II were analyzed by GC and GC-MS. ar-Turmerone, turmerone, and curlone were found to be the major compounds present in these fractions along with other oxygenated compounds.