ABSTRACT
DcMaster is a family of PIF/Harbinger-like class II transposable elements identified in carrot. We present a modified Transposon Display molecular marker system allowing amplification of genomic regions containing DcMaster elements. We scored 77 DcMaster Transposon Display (DcMTD) amplicons, of which 54 (70%) were segregating in the F(2) progeny from the cross between wild and cultivated carrot. Segregating amplicons were incorporated into a previously developed molecular linkage map of carrot. Twenty-eight markers were attributed to the wild parent, 23 originated from the cultivated parent, and three markers remained unlinked. The markers were evenly distributed among the nine linkage groups. However, differences in the distribution pattern of DcMaster insertion sites in the genomes of the wild and cultivated parent were observed. Specificity of the obtained amplicons was confirmed by sequencing and three putative DcMaster subfamilies, differing in the sequence of their terminal inverted repeats, were revealed.
Subject(s)
DNA Transposable Elements , Daucus carota/genetics , Base Sequence , Chromosome Mapping , DNA Primers/genetics , DNA, Plant/genetics , Genetic Markers , Genome, Plant , Molecular Sequence Data , Polymorphism, GeneticABSTRACT
We applied the RFLP approach to identify the cytoplasmic genotypes of selected onion breeding materials from Poland. For this purpose, mitochondrial DNA from cytoplasmic male-sterile (CMS) and male-fertile onions were hybridized with the probes for the following mitochondrial genes: atpA, atp6, atp9, cob, cox1, nad3, nad4 and nad6. S-, T- or C-cytoplasm was represented in each analyzed sterile accession. Some new polymorphisms shared by S- and C-cytoplasmic onions were identified. We also used currently available PCR markers to test if cytoplasmic heterogenity occurs within onion inbreds. A fraction of the plants bearing S-cytoplasm were found within two male-fertile lines, but such plants were not detected in the open-pollinated cultivars Sochaczewska, Wolska and Zytawska. Both the RFLP and PCR approaches gave some proof of existing mitochondrial heteroplasmy in onions
