Subject(s)
Bacteriophage Typing , Salmonella Infections/microbiology , Salmonella Phages/physiology , Salmonella paratyphi A/classification , Salmonella typhi/classification , Salmonella typhimurium/classification , Salmonella/classification , Salmonella/virology , Humans , India , Salmonella/isolation & purification , Salmonella paratyphi A/isolation & purification , Salmonella paratyphi A/virology , Salmonella typhi/isolation & purification , Salmonella typhi/virology , Salmonella typhimurium/isolation & purification , Salmonella typhimurium/virologySubject(s)
Anti-Bacterial Agents/pharmacology , Cross Infection/drug therapy , Drug Resistance, Multiple , Staphylococcal Infections/drug therapy , Staphylococcus/drug effects , Anti-Bacterial Agents/therapeutic use , Coagulase/metabolism , Cross Infection/epidemiology , Humans , India/epidemiology , Microbial Sensitivity Tests , Staphylococcal Infections/epidemiology , Staphylococcus/classification , Staphylococcus/metabolismABSTRACT
A total of two hundred blood samples collected from patients presenting with febrile illness were followed by culture using traditional and biphasic culture methods. Use of biphasic medium was observed to be of dual advantage as it grew pathogens earlier (28.5 percent in 18 hours, 63 percent in 36 hours of incubation) as well as in more number of cultures i.e. higher isolation rate (78 percent) when compared to traditional method.
Subject(s)
Bacteremia/diagnosis , Bacteriological Techniques , Bacteremia/microbiology , Bacteria/drug effects , Bacteria/isolation & purification , Culture Media , Drug Resistance, Microbial , Evaluation Studies as Topic , HumansABSTRACT
Twenty two HIV antibody ELISA reactive serum samples from blood donors were subjected to Western blot (WB) test, out of which 12 sera were found WB positive, five were WB-indeterminate and five were WB-negative. Electrophoresis on agar gel and polyacrylamide gel (PAG) was done in all ELISA reactive sera. Two of the 12 WB-positive and one of the five WB-indeterminate donors showed presence of monoclonal (M) immunoglobulin component and which was identified as of IgG isotype on immunoelectrophoresis (IEP). Sera with 'M' component demonstrated decreased levels of IgA and IgM whereas other sera showed polyclonal increase of immunoglobulins. The paraproteins observed in these patients are possibly a reflection of B-cell overactivation.
Subject(s)
HIV Seropositivity/complications , Paraproteinemias/etiology , Humans , ImmunoelectrophoresisABSTRACT
The occurrence of G. vaginalis in patients with non-specific vaginitis (NSV) was studied. Of the 200 women with vaginitis screened, 84 were diagnosed to have non-specific vaginitis. G. vaginalis was isolated from 33 (39.28%) patients either alone or in combination with other organisms, while one of the healthy controls with no vaginal discharge yielded G. vaginalis on culture. The nature of vaginal discharge could not be correlated with the type of infection. Clue cells and amine test gave inconsistent results in the presence of G. vaginalis infection. A varying susceptibility pattern of G. vaginalis was observed against various antimicrobials, metronidazole (95%) and gentamicin (75%) showing highest sensitivity.
Subject(s)
Gardnerella vaginalis/isolation & purification , Haemophilus Infections/diagnosis , Pregnancy Complications, Infectious/diagnosis , Vaginitis/diagnosis , Female , Humans , Incidence , PregnancySubject(s)
Lung Diseases, Obstructive/blood , alpha 1-Antitrypsin/analysis , Adolescent , Adult , Child , Female , Humans , Male , Middle AgedABSTRACT
In our earlier study (Yemul et, al, 1983) we have reported elevation of serum alpha one antitrypsin levels in patients of lepromatous leprosy and lepra reaction. In this study estimation of serum alpha one antitrypsin levels in fifty lepra reaction patients (8 of type 1 and 42 of type II) and fifty age and sex matched healthy controls is described. Alpha one antitrypsin levels were elevated in lepra reaction patients (type I--mean value of 332 mg% and S.D. +/- 118.8 and type II--mean value of 450 mg% and S.D. +/- 73.7) when compared with the healthy controls (mean value of 285 mg% and S.D. +/- 66.05). The increase in levels in type II lepra reaction patients was statistically significant. The results are discussed to correlate the increased levels of alpha one antitrypsin and the high bacterial load leading to the release of various proteases in type II lepra reaction.
