ABSTRACT
Onychomycosis is a common nail problem in our country. Diabetic patients are more prone to develop onychomycosis. Various types of fungi are the causative agents of nail infections. This cross sectional explorative study was designed to find out the pattern of onychomycosis among diabetic and non-diabetic patients attending the out patient Department of Dermatology and Venereology and Endocrinology of Bangabandhu Sheikh Mujib Medical University Hospital, Dhaka, Bangladesh from July 2012 to June 2013. Clinically diagnosed patients of onychomycosis with diabetic or non-diabetic were included purposively in this study. Scraping or clipping from infected nail materials were processed for microscopy and culture in Sabouraud's dextrose agar media and Dermatophyte test media. Clinical features, microscopic examination results and culture interpretations were recorded and compared in two groups. A total of 87 clinically diagnosed patients of onychomycosis were included in this study. Out of 87 patients of onychomycosis, 54 patients were diabetic and 33 patients were non-diabetic. Trichophyton rubrum and Trichophyton mentagrophytes were found in 24(44.44%) and 19(35.18%) diabetic patients. Candida albicans and non-albicanscandida species were found in 1(1.85%) and 2(3.70%) diabetic patients. On the other hand, Trichophyton rubrum and Trichophyton mentagrophytes were found in 1(3.03%) and 2(6.06%) non-diabetic patients. Candida albicans and non-albicanscandida species were found in 8(24.24%) and 8(24.24%) non-diabetic patients. Growth of fungus was found in 46(85.19%) diabetic patients which was significant (p=0.004) compared to that found in 19(57.58%) non-diabetic patients. No growth was found in 8(14.81%) diabetic and in 14(42.42%) non-diabetic patients. Dermatophytes were more found in diabetic patients and Candida albicans and non-albican Candida spp. were more found in non-diabetic patients. So, the pattern of onychomycosis was different in diabetics compared to non-diabetics. Further study may be done with large number of sample to determine more accurate pattern of onychomycosis among diabetics.
Subject(s)
Diabetes Complications , Foot Dermatoses , Fungi , Hand Dermatoses , Nails/microbiology , Onychomycosis , Aged , Bangladesh/epidemiology , Cross-Sectional Studies , Diabetes Complications/diagnosis , Diabetes Complications/epidemiology , Diabetes Complications/microbiology , Female , Foot Dermatoses/diagnosis , Foot Dermatoses/epidemiology , Foot Dermatoses/microbiology , Fungi/classification , Fungi/isolation & purification , Hand Dermatoses/diagnosis , Hand Dermatoses/epidemiology , Hand Dermatoses/microbiology , Humans , Male , Middle Aged , Mycology/methods , Onychomycosis/diagnosis , Onychomycosis/epidemiology , Onychomycosis/etiologyABSTRACT
Alopecia areata is a common disorder affecting any of the hair bearing areas of the body. Most of the cases are diagnosed on the clinical ground only. Histopathological changes may help in the diagnosis in difficult cases. The present study was designed to observe the histopathologic changes of alopecia areata at different stages. Thirty consecutive patients suffering from alopecia areata were enrolled. Face to face interview was performed using a structured pre-tested questionnaire. At the initial visit, one 4mm punch scalp biopsy specimen was taken from an area of hair loss. Biopsy specimens were sent in a test tube filled with formalin to the department of Pathology, Bangabandhu Sheikh Mujib Medical University (BSMMU) where tissue was processed, sectioned and slides were prepared. Haematoxylin and eosin stains were used. Aanagen hairs were decreased in all stages of alopecia areata. Telogen hairs increased in acute and chronic stages whereas catagen hairs increased markedly in sub-acute stage. Although miniaturized (atrophic) follicle was frequently found in chronic stage. It was absent in acute and sub-acute stages. Moderate to dense peribulbar infiltration of lymphocytes were observed in acute stage and mild to moderate infiltration in sub-acute stage. In chronic stage either no or mild infiltrations were observed. Peribulbar infiltration of eosinophils and macrophages were seen in all stages of alopecia areata. Alopecia areata can be diagnosed with some confidence, even when inflammatory infiltrate is absent, based on increased numbers of telogen hairs in the acute and chronic stages, increased miniaturized hairs in chronic stage and markedly increased catagen hairs in sub-acute stage.
Subject(s)
Alopecia Areata/pathology , Adolescent , Adult , Alopecia Areata/etiology , Alopecia Areata/therapy , Bangladesh , Cohort Studies , Cross-Sectional Studies , Female , Humans , Male , Middle Aged , Young AdultABSTRACT
A prospective experimental study was conducted in a postgraduate institute in Dhaka, Bangladesh, from April '05 to September '05. A total of 214 doctors of a postgraduate medical institute were included in the study who was involved in research works. A written questionnaire was given to the respondents to assess their knowledge, attitude and practice about informed consent (IC). The data was analyzed statistically. Chi-Square test was done to see the level of significance. Regarding knowledge of IC, we found maximum participants were more or less knowledgeable about it. The attitude of the respondents was also in favour of taking IC. In practice, most of them had taken informed consent that was verbal (39.3%) and written (47.6%). But some of the researcher (13.1%) had not taken any informed consent. By the present study, it may be concluded that the researchers of the concerned institute were aware of IC.
