ABSTRACT
Organelles, cells, tissues, or any other biological construction can be preserved using a method called cryopreservation, in which samples are cooled to extremely low temperatures. The reaction of the living cell to the formation of ice is both theoretically intriguing and practically useful. Since osmotic shock, membrane damage, and ice crystal formation during freezing and thawing will result in cell death, other viable tissues and stem cells, which are of much importance for uses in basic research and medical applications, may not be preserved by simple cooling or freezing for large periods. With the aid of cryoprotective agents (CPAs) and temperature control technology, the successful cryopreservation of cells and tissues have been rising in recent time. Sometimes excessive use of cryoprotective agents may damage the original structure of preserved tissue. Therefore, cryoprotective agents should be used appropriately, and their quantities should be regulated. Excessive cooling may damage the membrane structure of the cell, so cooling should be done appropriately. Slow freezing and vitrification method are the two procedures that may be used for cryopreservation. Vitrification's main benefit is that it significantly reduces the likelihood of freeze damage, making it possible to maintain a high enough cell survival rate. Good manipulation skills are also required, and there is a considerable risk of infection with pathogenic pathogens. [As1] Fruitful cryopreservation of cells or tissues and their therapeutic use will require ongoing knowledge of the physical and chemical features which take place in the freezing and thawing cycle. We briefly discuss representative cryopreservation techniques and their clinical uses in this study.
