Process Intensification of Enzymatic Synthesis of Flavor Esters: A Review.

The conventional flavor synthesis method suffers from low yields, time inefficiency, and extreme reaction conditions. Therefore, there is a necessity for the green and novel synthesis approach to overcome these limitations. The current review presents a holistic insight into different aspects associated with the synthesis of flavor esters using the immobilized enzyme. The application of process intensification tools such as ultrasound and microwave irradiation can enhance the reaction efficiency because of higher product recovery, less formation of by-products, and decreased energy consumption. This review presents the process intensification of value-added flavor esters synthesis and the mechanism of ultrasound and microwave action on the enzyme to enhance the enzyme activity and increase the reaction rate. It also summarizes the role of process intensification in enzymatic flavor ester synthesis, followed by specific examples as reported in the literature.

Intensification of Enzymatic Synthesis of Decyl Oleate Using Ultrasound in Solvent Free System: Kinetic, Thermodynamic and Physicochemical Study.

The present study evaluates the potential use of ultrasound irradiation to synthesize decyl oleate using Fermase CALBTM10000 under the solvent-free system (SFS). The optimal condition to achieve a maximum yield of 97.14% was found to be 1:2 oleic acid:decanol ratio, 1.8% (w/w) enzyme loading, 45°C temperature, 200 rpm agitation speed, 50 W power input, 50% duty cycle, 22 kHz frequency and reaction time of 25 minutes. The thermodynamic study was done to determine the change in entropy, Gibb's free energy, and change in enthalpy at various temperatures. The experimental results and kinetic study showed that the reaction followed ordered bi-bi model with kinetic parameters as rate of reaction (V max ) = 35.02 M/min/g catalyst, Michaelis constant for acid (K A ) = 34.47 M, Michaelis constant for alcohol (K B ) = 3.31 M, Inhibition constant (Ki) = 4542.4 M and sum of square error (SSE) = 0.000334. The application of ultrasound irradiation combined with biocatalyst and the absence of solvent intensified the process compared to the traditional stirring method using hexane as solvent.

Acoustic cavitation promoted lipase catalysed synthesis of isobutyl propionate in solvent free system: Optimization and kinetic studies.

The present work highlights the effect of ultrasound on enzymatic synthesis of isobutyl propionate, a rum flavor by esterification of isobutanol and propionic acid in non-aqueous, solvent free system (SFS) using Fermase CALB™10000. The optimization study for different variables in presence of ultrasound showed a maximum conversion of 95.14% at 60°C temperature, 4% w/w enzyme dose, 1:3 acid:alcohol ratio, 40W power, 25kHz frequency, 50% duty cycle and 150rpm speed in 3h as compared to 10h of conventional method. The optimal enzyme loading was reduced to 4% w/w using ultrasound irradiation compared to 5% w/w of conventional. The efficiency of enzyme improved notably and can be reused up to seven cycles preserving its former activity. The application of ultrasound greatly enhanced esterification reactions by maintaining enzyme stability and improving the production yield. Moreover, owing towards a green approach, the synthesis is carried out in SFS for development of lucrative flavor ester. Bisubstrate kinetic models like random bi-bi, ping pong bi-bi and ordered bi-bi were applied to the experimental data using non-linear regression analysis. The experimental data and kinetic study revealed that reaction obeyed Ping-Pong bi-bi model with kinetic parameters, Vmax=50.0M/min/gcatalyst, KA=4.87×10-2M, KB=9.06×10-4M, KiA=9.8×10-1M, KiB=1.05×10-3M &SSE=2.74×10-4 for lipase catalysed synthesis of isobutyl propionate under ultrasound with inhibition by both acid and alcohol.