[Gas-mass spectrometry in the identification and determination of ethanol in blood]. / Primena gasno-masene spektrometrije u identifikaciji i odredivanju etanola u krvi.

A contemporary analytical method for positive identification and quantitative toxicological--chemical analysis of ethanol in blood samples using the methods of gas extraction, thermal adsorption/desorption and gas-mass spectrometry, was developed. Maximal sensitivity of ethanol detection was 0.27 mmol/l.

Endogenous regulation of endothelial cell proliferation.

Bovine aortic endothelial cells (BAEC) in culture have the ability to regulate their own proliferation. We have found that a fraction below 100,000 daltons obtained from the media of confluent cultures of BAEC inhibits tritiated thymidine [3H]TdR incorporation as well as their proliferation. The inhibition is dose- and time-dependent; maximum inhibition of [3H]TdR incorporation occurs 8 hr after cells are released from synchronization and the inhibitory fraction is added. Inhibition is evident at concentrations as low as 50 micrograms/ml and reaches a maximum at 600 micrograms/ml. The blockage of [3H]TdR incorporation is reflected in the inhibition of cell proliferation. In the presence of 400 micrograms of endogenous inhibitor per ml of media, added at the time of plating, the average population doubling time increases from 19 to 41 hr. These findings indicate that, in culture, BAEC can regulate their own proliferation by synthesizing an endogenous inhibitor(s) of proliferation.