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1.
Pediatr Pol ; 70(10): 803-8, 1995 Oct.
Article in Polish | MEDLINE | ID: mdl-8649926

ABSTRACT

Systematic quality assessment of cytogenetic studies is very important for maintaining high standards of diagnostic testing and adequate genetic service. Results of quality assessment for 714 pre- and 907 postnatal cytogenetic studies performed in the Genetic Department of the National Research Institute of Mother and Child are presented. The assessment included: reporting time, quality of chromosome preparations, reliability and success rate of the studies. Mean reporting time for blood samples was 20 days and for amniotic fluid 23 days. Chromosome banding quality was adequate to reasons for referral in 86.9% of blood samples and in 100% of amniotic fluids. The success rate for pre- and postnatal studies was 99.4 and 96.6%, respectively. The need to develop a quality assessment scheme for clinical cytogenetics in Poland is discussed.


Subject(s)
Diagnostic Services/standards , Genetic Techniques/standards , Evaluation Studies as Topic , Genetic Testing/standards , Humans , Poland , Prenatal Diagnosis/standards , Quality Control , Reproducibility of Results
2.
Ginekol Pol ; 64(4): 204-9, 1993 Apr.
Article in Polish | MEDLINE | ID: mdl-8282244

ABSTRACT

Prenatal diagnosis of chromosomal abnormalities by chorionic villus (CVS) in the first trimester of pregnancy is presented. Trophoblast biopsy techniques, methods of cytogenetic analysis of this tissue as well as technical and diagnostic problems in fetal karyotyping are described.


Subject(s)
Chromosome Aberrations/diagnosis , Fetal Diseases/diagnosis , Prenatal Diagnosis , Trophoblasts/cytology , Biopsy , Chorionic Villi Sampling , Chromosome Disorders , Cytogenetics , Female , Humans , Karyotyping , Pregnancy , Pregnancy Trimester, First
3.
Pol Tyg Lek ; 45(38-39): 773-7, 1990.
Article in Polish | MEDLINE | ID: mdl-2095544

ABSTRACT

Characteristics of the chromosomal aberrations diagnosed in 959 prenatal tests in the II trimester of pregnancy is presented. Chromosomal aberrations were diagnosed in 33 tests (3.4%). Twenty one out of these aberrations (2.2%) were of labile character. Six aberrations resulted from the parental segregation, translocation or chromosomal inversion. In 12 cases fetus inherited stable aberration from one of parents. It amounted to 1.2% of all tested cases. Chromosomal aberrations were diagnosed in 2.7% cases tested due to the risk related to the mother's age. Half of them was trisomy of chromosome 21. Chromosomal aneuploidy in the progeny of families with a child with the same abnormality was diagnosed in 1.6% of cases. Chromosomal mosaicism was diagnosed in 2.2% of cases including 0.2% of cases with true mosaicism and 1.98% of cases with pseudomosaicism. Incidence and type of the diagnosed chromosomal aberrations coincided with foreseen aberrations for each group of the genetic risk.


Subject(s)
Amniotic Fluid/cytology , Chromosome Aberrations/genetics , Adult , Amniocentesis/methods , Chromosome Aberrations/diagnosis , Chromosome Aberrations/prevention & control , Chromosome Disorders , Female , Genetic Counseling/methods , Humans , Maternal Age , Pregnancy , Pregnancy Trimester, Second , Pregnancy, High-Risk , Risk Factors
4.
Ginekol Pol ; 60(7-9): 373-9, 1989.
Article in Polish | MEDLINE | ID: mdl-2702196

ABSTRACT

Laboratory problems and diagnostic difficulties are analysed in 1135 prenatal cytogenetic examinations of amniotic fluid cells in the Genetics Laboratory of the Mother and Child Institute. The effectiveness and the reliability of the method for the purposes of prenatal diagnosis of chromosomal aberrations were evaluated. They were comparable to those reported from other similar centres in the world.


Subject(s)
Chromosome Aberrations/genetics , Prenatal Diagnosis/methods , Amniocentesis/methods , Amniotic Fluid/cytology , Female , Humans , Karyotyping , Pregnancy , Pregnancy Trimester, Second , Reproducibility of Results
5.
Article in English | MEDLINE | ID: mdl-2896562

ABSTRACT

1. Some haematological and biochemical blood parameters in female Indian elephants were investigated. 2. Haematological data were as follows: Ht = 39.2 +/- 2.36%, Hb = 10.1 +/- 0.54 g%, RBC = 2.66 +/- 0.32 x 10(6)/mm3, WBC = 5.43 +/- 0.48 x 10(3)/mm3. Lymphocytes, determined on blood smears were mainly leucocytes from (67.0 +/- 1.59%). Data for MCV, MCH and MCHC are also given. 3. Blood plasma was separated into 5 main fractions, total plasma protein concentration was 6.98 +/- 0.53 g%, A/G ratio was 0.69 +/- 0.1. 4. Plasma minerals concentration was as follows: Na, 3044 +/- 194 micrograms/ml; K, 529 +/- 38.5 micrograms/ml; Mg, 33.0 +/- 3.43 micrograms/ml; Ca, 181.0 +/- 17.8 micrograms/ml; InP, 44.6 +/- 6.1 micrograms/ml. Ca: P ratio was 3.25 +/- 0.34. 5. Some seasonal differences in investigated parameters were observed. Ht values, WBC and neutrophils number as well as Ca, and Mg concentrations were higher during winter, whereas RBC and Na and InP concentrations were lower in winter.


Subject(s)
Elephants/blood , Animals , Animals, Domestic , Blood Proteins/analysis , Electrolytes/blood , Erythrocyte Count , Female , Hematocrit , Hemoglobins/analysis , Leukocyte Count , Seasons
6.
Article in English | MEDLINE | ID: mdl-2882892

ABSTRACT

Plasma lysozyme activity changed during the 2 years of the investigation period. In some species (e.g. ponies and goats) there are only seasonal fluctuations with the highest levels during summer and autumn in ponies and during winter in goats. In two Camelidae species, guanacos and llamas, only, downward trends were found, whereas in donkeys, both types of changes: seasonal variations with the maximal level during autumn and an upward long-term trend were found.


Subject(s)
Artiodactyla/immunology , Camelids, New World/immunology , Goats/immunology , Horses/immunology , Muramidase/blood , Animals , Female , Male , Muramidase/immunology , Periodicity , Seasons , Species Specificity
7.
Article in English | MEDLINE | ID: mdl-2435451

ABSTRACT

Twenty-four hour changes in lysozyme level, total plasma protein concentration, gamma-globulin concentration, leucocyte number and different forms of leucocyte in the peripheral blood of male chinchillas and rabbits were investigated. All investigated parameters in both species showed statistically significant 24-hr changes, but only total plasma protein concentration in rabbits and gamma-globulin concentration and leucocyte number in chinchillas have a circadian rhythm character. The cosine curve characteristics for these rhythms are as follows; acrophases 0.45 + 2.18 hr, 10.02 + 4.29 hr, 2.14 + 3.20 hr; amplitudes 0.48 + 0.32 g%, 0.34 + 0.41 g%, 1.12 + 0.88 X 10(3)/min3; mesors 6.10 + 0.22 g%, 2.23 + 0.21 g%, 8.78 + 0.65 X 10(3)/mm3, respectively.


Subject(s)
Blood Proteins/metabolism , Chinchilla/blood , Circadian Rhythm , Granulocytes/cytology , Leukocyte Count , Lymphocytes/cytology , Muramidase/blood , Rabbits/blood , Animals , Male , Species Specificity , gamma-Globulins/metabolism
8.
Article in English | MEDLINE | ID: mdl-2864198

ABSTRACT

In 34 pure breed Arabian horses divided into four groups (Gr. I--10 pregnant mares, Gr. II--7 barren mares, Gr. III--10 foals born in 1981, Gr. IV--7 foals born in 1982) seasonal changes in total blood serum protein, its electrophoretic fractions and the activity of AspAT and AlAT were studied. Seasonal cyclicity was found in all groups in the amount of total serum proteins, and alpha 2- and beta 1-globulin fractions. Cyclicity was found in the level of albumin and activity of AspAT in three groups, not Gr. II, and in gamma-globulin, not Gr. IV. beta 2-globulin and AlAT cyclicity was found in two groups and alpha 1-globulin cyclicity was found only in Gr. II. Out of nine indices studied, cyclicity was found in eight of them in pregnant mares, Gr. I; in seven in older foals, Gr. III; in six in the young foals, Gr. IV; and in five in barren mares, Gr. II.


Subject(s)
Acclimatization , Alanine Transaminase/blood , Aspartate Aminotransferases/blood , Horses/growth & development , Animals , Blood Proteins/analysis , Female , Horses/blood , Periodicity , Seasons , Serum Albumin/analysis , Serum Globulins/analysis
9.
Article in English | MEDLINE | ID: mdl-2866888

ABSTRACT

Changes in some trace minerals concentrations (calcium, inorganic phosphorus, magnesium, zinc, iron, copper, sodium and potassium) in blood plasma were investigated during a three-year period in Shetland pony mares. Blood plasma mineral concentrations were determined by the atomic absorption method and colorimetric method. The three-year averages were in micrograms/ml; Na 4630 +/- 168; K 277 +/- 3.8; Ca 171 +/- 3.8; P 31.5 +/- 0.74; Fe 1.92 +/- 0.14; Zn 1.07 +/- 0.04 and Cu 1.06 +/- 0.02. Two trace minerals (magnesium in inorganic phosphorus) showed only long-term tendency changes--upward or downward trends were not connected with the season. Most of the investigated minerals showed both a long-term change tendency and more or less marked seasonally related concentration changes, being higher during autumn or winter (zinc, sodium), or during spring and summer (calcium, copper, iron and potassium). Some interrelationships between particular trace mineral concentrations in blood plasma were also observed.


Subject(s)
Horses/blood , Trace Elements/blood , Animals , Calcium/blood , Copper/blood , Female , Iron/blood , Magnesium/blood , Phosphates/blood , Potassium/blood , Seasons , Sodium/blood , Spectrophotometry, Atomic , Zinc/blood
10.
Article in English | MEDLINE | ID: mdl-2863038

ABSTRACT

In 34 pure-breed Arabian horses divided into four groups (Gr. I, ten pregnant mares; Gr. II, seven barren mares; Gr. III, ten foals born in 1981; Gr. IV, seven foals born in 1982) seasonal changes in the white blood cell system, cortisol level and lyzozyme activity were studied. Seasonal periodicity was found in all groups for the number of lymphocytes, segmented neutrophils and eosinophils and cortisol level. Leukocyte periodicity was found in three groups, but not in the barren mares. In lyzozyme activity there was periodicity in three groups but not in the youngest foals. In the stab neutrophils, basophils and monocytes no cycle was observed. The behaviour of the indices studied showed the influence of age of the horses (mature vs young) and the physiological state of the mares (pregnancy or barrenness).


Subject(s)
Horses/blood , Hydrocortisone/blood , Leukocyte Count , Muramidase/blood , Animals , Eosinophils/cytology , Female , Leukocytes/cytology , Periodicity , Pregnancy , Seasons
11.
Article in English | MEDLINE | ID: mdl-6149060

ABSTRACT

Changes in nine haematological indices in adult female and male chinchilla were investigated over a period of 18 months. All haematological values were in the same range both for females and males, although seasonal fluctuations were more evident in the females. High levels of the red blood cells count (RBC), haemoglobin concentration (Hb), mean cell haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) during the winter months and low levels of these parameters during the summer months were found. The white blood cells count (WBC) and lymphocyte number (L) also decreased in summer, their values increasing during autumn and spring. The haematocrit values (Ht) and mean cell volumes (MCV) are high during the summer and low during the winter.


Subject(s)
Chinchilla/blood , Seasons , Animals , Environment , Erythrocyte Count , Erythrocyte Indices , Female , Hematocrit , Hemoglobins/metabolism , Leukocyte Count , Male , Sex Characteristics
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