ABSTRACT
We studied the role of the additives trehalose and poly(vinyl pyrrolidone) in the physical and pharmacokinetic properties of peptide drug incorporated hyaluronic acid microneedles. Poly(vinyl pyrrolidone) increases the mechanical strength of microneedles and ameliorates drug bioavailability in vivo, suggesting that poly(vinyl pyrrolidone) can be a promising additive in the fabrication of peptide drug-encapsulated fully dissolving microneedles.
Subject(s)
Drug Delivery Systems , Hyaluronic Acid/administration & dosage , Needles , Parathyroid Hormone/administration & dosage , Povidone/administration & dosage , Trehalose/administration & dosage , Animals , Hyaluronic Acid/chemistry , Hyaluronic Acid/pharmacokinetics , Microinjections , Parathyroid Hormone/blood , Parathyroid Hormone/chemistry , Parathyroid Hormone/pharmacokinetics , Povidone/chemistry , Povidone/pharmacokinetics , Rats , Trehalose/chemistry , Trehalose/pharmacokineticsABSTRACT
Tissue engineered biomaterials have biodegradable and biocompatible properties. In this study, we have fabricated sponges using duck's feet derived collagen (DC) and gellan gum (GG), and further studied its inflammatory responses. The as-prepared duck's feet DC/GG sponges showed the possibility of application as a tissue engineering material through in vitro and in vivo experiments. The physical and chemical properties of sponges were characterized by compression strength, porosity, and scanning electron microscopy, etc. In vitro cell viability were investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. An inflammatory response was studied after seeding RAW264.7 cells on as-fabricated sponges using reverse transcriptase-polymerase chain reaction. In vivo studies were carried out by implanting in subcutaneous nude mouse followed by extraction, histological staining. Collectively, superior results were showed by DC/GG sponges than GG sponge in terms of physical property and cell proliferation and thus can be considered as a potential candidate for future tissue engineering applications.
