ABSTRACT
In our systematic screening programme for marine actinomycetes, a bioactive Streptomycete was isolated from marine sediment samples of Bay of Bengal, India. The taxonomic studies indicated that the isolate belongs to Streptomyces chibaensis and it was designated as S. chibaensis AUBN1/7. The isolate yielded a cytotoxic compound. It was obtained by solvent extraction followed by the chromatographic purification. Based on the spectral data of the pure compound, it was identified as quinone-related antibiotic, resistoflavine (1). It showed a potent cytotoxic activity against cell lines viz. HMO2 (Gastric adenocarcinoma) and HePG2 (Hepatic carcinoma) in vitro and also exhibited weak antibacterial activities against Gram-positive and Gram-negative bacteria.
Subject(s)
Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/toxicity , Benzopyrenes/isolation & purification , Benzopyrenes/toxicity , Streptomyces/chemistry , Anti-Bacterial Agents/pharmacology , Benzopyrenes/pharmacology , Cell Line, Tumor , Chromatography , Geologic Sediments/microbiology , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Humans , India , Microbial Sensitivity Tests , Molecular Structure , Spectrum Analysis , Streptomyces/classification , Streptomyces/isolation & purification , Streptomyces/physiologyABSTRACT
In our systematic screening programme for marine actinomycetes, a bioactive streptomycete was isolated from marine sediment samples of the Bay of Bengal, India. The isolate yielded a new cytotoxic compound. This was obtained by solvent extraction followed by chromatographic purification. The pure compound was identified from spectroscopic data as a quinone-related antibiotic, 1-hydroxy-1-norresistomycin (1). It showed a potent cytotoxic activity against cell lines viz. HMO2 (gastric adenocarcinoma) and HePG2 (hepatic carcinoma) in vitro. It also exhibited antibacterial activities against Gram-positive and Gram-negative bacteria.
