ABSTRACT
The unique structure of G4.0 PAMAM dendrimers allows a drug to be enclosed in internal spaces or immobilized on the surface. In the conducted research, the conditions for the formation of the active G4.0 PAMAM complex with doxorubicin hydrochloride (DOX) were optimized. The physicochemical properties of the system were monitored using dynamic light scattering (DLS), circular dichroism (CD), and fluorescence spectroscopy. The Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) method was chosen to determine the preferential conditions for the complex formation. The highest binding efficiency of the drug to the cationic dendrimer was observed under basic conditions when the DOX molecule was deprotonated. The decrease in the zeta potential of the complex confirms that DOX immobilizes through electrostatic interaction with the carrier's surface amine groups. The binding constants were determined from the fluorescence quenching of the DOX molecule in the presence of G4.0 PAMAM. The two-fold way of binding doxorubicin in the structure of dendrimers was visible in the Isothermal calorimetry (ITC) isotherm. Fluorescence spectra and release curves identified the reversible binding of DOX to the nanocarrier. Among the selected cancer cells, the most promising anticancer activity of the G4.0-DOX complex was observed in A375 malignant melanoma cells. Moreover, the preferred intracellular location of the complexes concerning the free drug was found, which is essential from a therapeutic point of view.
Subject(s)
Dendrimers , Doxorubicin , Dendrimers/chemistry , Doxorubicin/chemistry , Doxorubicin/pharmacology , Humans , Cell Line, Tumor , Drug Carriers/chemistry , Drug Delivery Systems/methods , Drug Liberation , Cell Survival/drug effectsABSTRACT
In the present paper, new pyrimidine derivatives were designed, synthesized and analyzed in terms of their anticancer properties. The tested compounds were evaluated in vitro for their antitumor activity. The cytotoxic effect on normal human dermal fibroblasts (NHDF) was also determined. According to the results, all the tested compounds exhibited inhibitory activity on the proliferation of all lines of cancer cells (colon adenocarcinoma (LoVo), resistant colon adenocarcinoma (LoVo/DX), breast cancer (MCF-7), lung cancer (A549), cervical cancer (HeLa), human leukemic lymphoblasts (CCRF-CEM) and human monocytic (THP-1)). In particular, their feature stronger influence on the activity of P-glycoprotein of cell cultures resistant to doxorubicin than doxorubicin. Tested compounds have more lipophilic character than doxorubicin, which determines their affinity for the molecular target and passive transport through biological membranes. Moreover, the inhibitory potential against topoisomerase II and DNA intercalating properties of synthesized compounds were analyzed via molecular docking.
Subject(s)
Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Pyrimidines/chemistry , Pyrimidines/pharmacology , ATP Binding Cassette Transporter, Subfamily B, Member 1/metabolism , Cell Cycle/drug effects , Cell Line, Tumor , Cell Movement/drug effects , Cell Proliferation/drug effects , Dose-Response Relationship, Drug , Humans , Molecular Conformation , Molecular Docking Simulation , Molecular Dynamics Simulation , Molecular Structure , Structure-Activity RelationshipABSTRACT
Mucin 7 (encoded byMUC7) is a human salivary protein that has a role in the natural immune system. Fragments of mucin 7 exhibit antimicrobial activity against bacteria and yeast. Although the antimicrobial properties of peptides have been known and studied for decades, the exact mechanism of action of antimicrobial peptides (AMPs) is still unclear. It is known that some AMPs require divalent metal ions to activate their activity. Herein, we investigated three 15-mer MUC7 peptides, one of which (mother peptide, sequence, L3) is a synthetic analog of a fragment naturally excised from MUC7 (with His3, His8, and His 14) and its two structural analogs, containing only two histidine residues, His3, His13 and His8, His13 (L2 and L1, respectively). Since there is a correlation between lipophilicity, the presence of metal ions (such as Cu(II) and Zn(II)) and antimicrobial activity of AMP, antimicrobial properties of the studied peptides, as well as their complexes with Cu(II) and Zn(II) ions, were tested for activity against Gram-positive (Enterococcus faecalis, Staphylococcus epidermidis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria and fungi (Candida albicans). The results were correlated with their lipophilicity. Coordination and thermodynamic studies (potentiometry, UV-Vis, CD) revealed the formation of mainly mononuclear complexes in solution for all studied systems with different stability in the physiological pH range.
Subject(s)
Antimicrobial Peptides/pharmacology , Coordination Complexes/pharmacology , Mucins/pharmacology , Salivary Proteins and Peptides/pharmacology , Amino Acid Sequence , Antimicrobial Peptides/chemistry , Humans , Microbial Sensitivity Tests , Mucins/chemistry , Salivary Proteins and Peptides/chemistry , ThermodynamicsABSTRACT
Mucin 7 (called MUC7 or MG2) is a salivary protein whose fragments exhibit a strong antimicrobial activity and is a natural protection of organisms against pathogens. Up to date however the exact mechanism of their actions is unknown. One hypothesis covers an involvement of biologically occurring metal ions in this process. Herein, three 12-mer peptides have been included in the study, one of which (mother-peptide sequence, L3) is a direct analogue of a fragment naturally cut out from the MUC7 (with His3 and His8) and its two structural analogues, containing only one histidine residues His3 or His8 (L2 and L1, respectively), which are essential for binding to metal ions. Antimicrobial properties of the studied peptides as well as their complexes have been tested against bacterial and fungal strains revealing activity towards Gramm-positive bacteria (Enterococcus faecalis and Staphylococcus epidermidis) which were slightly enhanced upon Cu(II) or Zn(II) ions coordination. The study of both the biological and thermodynamic properties of considered systems has not been measured before. Exact coordination studies (potentiometry, ultraviolet/visible spectroscopy - UV-Vis, circular dichroism spectroscopy - CD) revealed the formation of mainly mono-nuclear complexes in solution for all studied systems in which the binding abilities turned out to be inversely proportional to the antimicrobial properties exhibited. However, distinctly different lipophilicity of peptides, the reduction of which (also upon metal coordination) increases the ability to inhibit the growth of E. faecalis and S. epidermidis strains, seems to be the most important factor of their activity.
Subject(s)
Anti-Infective Agents/pharmacology , Antimicrobial Cationic Peptides/pharmacology , Mucins/chemistry , Oligopeptides/pharmacology , Salivary Proteins and Peptides/chemistry , Anti-Infective Agents/chemistry , Antimicrobial Cationic Peptides/chemistry , Candida albicans/drug effects , Copper/chemistry , Enterococcus faecalis/drug effects , Humans , Hydrophobic and Hydrophilic Interactions , Oligopeptides/chemistry , Pseudomonas aeruginosa/drug effects , Streptococcus/drug effects , Zinc/chemistryABSTRACT
The binding mode provided by an unprotected peptide with non-coordinating side-chains is simple and well understood. However, when particular residues are inserted into the peptide sequence, they can have a significant impact on the stability of the formed complexes. The presence of non-bonding side chains of amino acids close to the metal binding centre in the peptide/protein can provide special interactions which result in increasing the stabilization of the formed species. Moreover, these interactions can play a crucial role in generating particular protein structures and in influencing biological activity. In the present paper it is shown how peptides with no specific predisposition for metal binding, like ANF peptides, can form metal complexes with a very high thermodynamic stability. For better understanding this peculiar behavior, a combined pH-metric and spectroscopic method was used to determine the stability and the solution structure of Cu(2+) and Ni(2+) complexes with NSFRY-NH(2) (ANF peptide) and a series of analogue peptides. All obtained data support the hypothesis that the complex-formation process is very similar for both metal ions and all the ligands, involving some intramolecular interactions among the different side chains. The two-dimensional NMR analysis of nickel complexes showed the occurrence of many inter-residue correlations and suggested the presence of a direct interaction between the d electrons of the metal ion and the π-ring system of the aromatic side-chains of the ligand.
Subject(s)
Copper/chemistry , Nickel/chemistry , Organometallic Compounds/chemistry , Peptide Fragments/chemistry , Amino Acid Sequence , Atrial Natriuretic Factor/chemistry , Magnetic Resonance Spectroscopy , Models, Molecular , Molecular Conformation , ProtonsABSTRACT
Multi-histidine peptide fragments of zebra-fish prion protein are effective ligands for Zn(II) ions. Moreover the formation of a dinuclear complex species with a longer peptide can suggest the existence of the cooperative effect in the metal ion binding.
Subject(s)
Prions/metabolism , Tandem Repeat Sequences , Zinc/metabolism , Animals , Cations, Divalent/metabolism , Potentiometry , Spectrometry, Mass, Electrospray Ionization , Zebrafish/metabolismABSTRACT
Alzheimer's disease is a fatal neurodegenerative disorder involving the abnormal accumulation and deposition of peptides (amyloid-beta, Abeta) derived from the amyloid precursor protein. Here, we present the structure and the Zn2+ binding sites of human and rat Abeta(1-28) fragments in water/sodium dodecyl sulfate (SDS) micelles by using 1H NMR spectroscopy. The chemical shift variations measured after Zn2+ addition at T>310 K allowed us to assign the binding donor atoms in both rat and human zinc complexes. The Asp-1 amine, His-6 Ndelta, Glu-11 COO-, and His-13 Nepsilon of rat Abeta28 all enter the metal coordination sphere, while His-6 Ndelta, His-13, His-14 Nepsilon, Asp-1 amine, and/or Glu-11 COO- are all bound to Zn2+ in the case of human Abeta28. Finally, a comparison between the rat and human binding abilities was discussed.