Sustainable utilization of unavoidable food waste into nutritional media for the isolation of bacterial culture for the removal of heavy metals.

This study aims to reuse food waste (FW) as growth media for bacterial cultures for bioremediation of heavy metal. The best natural medium was selected based on the carbon, nitrogen, and other elements. The batch culture of Comamonas terrae showed growth stability for 16 days in the pig bone medium. C. terrae showed the best growth at pH of 7.4, temperature of 35 °C, and medium concentration of 10 g/L. The C. terrae showed heavy metal (HM) removal efficiencies of Cd (52 %) Cr (63 %) Pb (62 %) and Zn (55 %). In addition, the Fourier transform infrared spectroscopy results revealed the bioadsorption of HM in C. terrae. The study suggests the C. terrae can efficiently remove HM and C. terrae may be used for bioremediation of HM. Therefore, pig bone waste is a cost-effective medium and a good solution for the valorization and reuse of FW in line with the circular economy.

Detection methods for sub-nanogram level of emerging pollutants - Per and polyfluoroalkyl substances.

Per- and polyfluoroalkyl substances (PFAS) are organofluorine compounds has been manufactured for more than five decades and used in different purposes. Among persistent organic pollutants, PFAS are toxic, bioaccumulative in humans, wildlife, and global environment. As per environmental protection agency (EPA) guidelines, the perfluorooctanoate and perfluorooctane sulfonate permissible limit was 0.07 ng/L in drinking water. When the concentration exceeds the acceptable limit, it has negative consequences for humans. In such a case, PFAS monitoring is critical, and a quick detection technique are highly needed. Health departments and regulatory agencies have interests in monitoring of PFAS presences and exposures. For the detection of PFAS, numerous highly precise and sensitive chromatographic methods are available. However, the drawbacks of analytical techniques include timely sample preparations and the lack of on-site applicability. As a result, there is an increasing demand for simple sensor systems for monitoring of PFAS in real field samples. In this review, we first describe the sample pre-treatment and analytical techniques for the detection of PFAS. Second, we broadly discussed available sensor system for the quantification of PFAS in different filed samples. Finally, future trends in PFASs sensor are also presented.

Influence of salinity on biofilm formation and COD removal efficiency in anaerobic moving bed biofilm reactors.

Anaerobic digestion is widely used for wastewater treatment, but this approach often relies on microbial communities that are adversely affected by high-salinity conditions. This study investigated the applicability of an anaerobic moving bed biofilm reactor (AMBBR) to treating high-salinity wastewater. The removal performance and microbial community were examined under salinity conditions of 1000-3000 mg/L, and a soluble chemical oxygen demand (sCOD) removal efficiency of up to 8% ± 2.74% was achieved at high-salinity. Scanning electron microscopy showed that microorganisms successfully attached onto the polyvinyl alcohol gel carrier, and the extracellular polymeric substances on the biofilm increased at higher salt concentrations. The AMBBR also maintained traditionally accepted levels of total alkalinity and volatile fatty acids for stable wastewater processing under these operating conditions. High-throughput sequencing indicated that Desulfomicrobium and three methanogenic groups were the dominant contributors to sCOD removal. Overall, the results showed that the AMBBR can successfully treat fish factory wastewater under varying salinity conditions.

Effect of biofilm formation on different types of plastic shopping bags: Structural and physicochemical properties.

Plastics and biofilms have a complicated relationship that has great interest. Bacterial cell attachment and biofilm formation is considered to cause health and environmental risks from plastic waste accumulation. In water, plastic waste could serve as a new substrate for bacteria. In our study, the attachment of Escherichia coli K12, to four types of plastic shopping bags (biodegradable polylactic acid and the non-biodegradable polypropylene, polyethylene and polyvinyl chloride) was investigated. The change in physicochemical phenomena of each plastic, such as reduced hydrophobicity and higher exopolysaccharide concentrations (total extractable protein and carbohydrate) resulted in increased biofilm content on the plastic surfaces. The bacterial colonization of different plastic surfaces controls the ionic strength of the nutrition sources. The adhesion of Escherichia coli K12 cells on the surfaces were revealed by SEM images. The finding shows that increases surface roughness, besides favor for adhesion of bacterial cells due to hydrophobicity leading to a rapid attachment of Escherichia coli K12 on the surfaces. In addition, we used Derjaguin-Landau-Verwey-Overbeek theory to predict the attachment of Escherichia coli K12, which gave result of adhesion due to the high energy barrier. This present study added to our knowledge of the possible consequences of plastics acting as a new habitat for microbes in different aquatic condition.

Mechanisms of chloride and sulfate removal from municipal-solid-waste-incineration fly ash (MSWI FA): Effect of acid-base solutions.

A general approach to managing municipal solid waste is by incineration. Unfortunately, large amounts of municipal-solid-waste-incineration fly ash (MSWI FA) is produced in the process, with their heavy metals content posing further problems to the environment. One fundamental treatment of MSWI FA heavy metals is called solidification-stabilization, where MSWI FA is solidified in cement-based materials to cap hazardous elements from being released into the environment. Mortar formed from this cement mixed with MSWI FA suffer from decreased compressive strength due to their chloride and sulfate contents. Thus, pre-treatment of MSWI FA to remove these salts before producing mortar is desirable. This study investigated treating MSWI FA with deionized water, 0.01 M and 0.1 M nitric acid, and 0.1 M and 0.25 M sodium carbonate to remove chloride and sulfate. Physical and chemical structures of treated and untreated MSWI FA was studied to understand the chloride and sulfate removal mechanisms. Treated MSWI FA was used as cement replacement in mortar, and the compressive strength was tested. Results suggest that all of the treatment solutions tested in this study can equally remove chloride (around 250,000 mg/kg), but sodium carbonate can remove sulfate at the highest extent (15,821 mg/kg). In addition, mortar with deionized-water-treated MSWI FA gave the highest compressive strength. Heavy metals leaching was tested by the Toxicity Characterization Leaching Procedure (TCLP) method, with results passing the standard.

The performance of vetivers (Chrysopogon zizaniodes and Chrysopogon nemoralis) on heavy metals phytoremediation: laboratory investigation.

Phytoremediation with vetiver was investigated in relation to heavy metal contaminated soil in Thailand. The work compared the performance of two species of vetiver named Songkhla 3 (Chrysopogon zizaniodes) and Prachuap Khiri Khan (Chrysopogon nemoralis) in absorbing lead, zinc, and cadmium in contaminated soils. Toxicity Characteristic Leaching Procedure (TCLP), and Allium tests were conducted to determine toxicity of treated soil. Ethylenediaminetetraacetic acid (EDTA) was also used to increase heavy metals concentration in solution in soil, which led to an increase in translocation and bioaccumulation factors. In general, results showed that concentration of heavy metals decreased in soil and increased in both the shoots and roots of vetivers during a 4-month treatment period. TCLP results indicated that the concentration of zinc and cadmium in contaminated soil was reduced over treatment time, and significantly increased after EDTA was applied. To confirm vetiver performance in phytoremediation, Allium testing showed that remained heavy metals in treated soils had no effect on nucleus aberration. Songkhla 3 and Prachuap Khiri Khan showed similar trends in their ability to remediate lead, zinc, and cadmium from contaminated soil. Both species could accumulate higher concentrations of heavy metals in their shoots and roots over time, and with EDTA application.

Leaching mechanisms of heavy metals from fly ash stabilised soils.

Fly ash is an industrial waste material that is repurposed as a soil stabiliser worldwide. In Thailand, many ground improvement projects utilise mixtures of cement and fly ash to stabilise weak soils. In this study, leaching mechanisms of arsenic, chromium, lead, and zinc from cement and fly ash stabilised soils were investigated in the laboratory. Leaching tests were performed, with different leachants and pH conditions, on cement and fly ash stabilised soils used for soil improvement in road embankment construction projects in Northern Thailand. The results suggested that chemical compounds (CaO and MgO) on fly ash surfaces can control the pH of the fly ash and soil leachant. The dissolution of chromium and zinc was found to be amphoteric and controlled by oxide minerals at a high or low pH. Arsenic leaching was found to be oxyanionic where AsO43- prevented the adsorption of arsenic onto the negatively charged fly ash surface. Different types of leachant also leached out in different amounts of heavy metals.

Response of Simulated Drinking Water Biofilm Mechanical and Structural Properties to Long-Term Disinfectant Exposure.

Mechanical and structural properties of biofilms influence the accumulation and release of pathogens in drinking water distribution systems (DWDS). Thus, understanding how long-term residual disinfectants exposure affects biofilm mechanical and structural properties is a necessary aspect for pathogen risk assessment and control. In this study, elastic modulus and structure of groundwater biofilms was monitored by atomic force microscopy (AFM) and optical coherence tomography (OCT) during three months of exposure to monochloramine or free chlorine. After the first month of disinfectant exposure, the mean stiffness of monochloramine- or free-chlorine-treated biofilms was 4 to 9 times higher than those before treatment. Meanwhile, the biofilm thickness decreased from 120 ± 8 µm to 93 ± 6-107 ± 11 µm. The increased surface stiffness and decreased biofilm thickness within the first month of disinfectant exposure was presumably due to the consumption of biomass. However, by the second to third month during disinfectant exposure, the biofilm mean stiffness showed a 2- to 4-fold decrease, and the biofilm thickness increased to 110 ± 7-129 ± 8 µm, suggesting that the biofilms adapted to disinfectant exposure. After three months of the disinfectant exposure process, the disinfected biofilms showed 2-5 times higher mean stiffness (as determined by AFM) and 6-13-fold higher ratios of protein over polysaccharide, as determined by differential staining and confocal laser scanning microscopy (CLSM), than the nondisinfected groundwater biofilms. However, the disinfected biofilms and nondisinfected biofilms showed statistically similar thicknesses (t test, p > 0.05), suggesting that long-term disinfection may not significantly remove net biomass. This study showed how biofilm mechanical and structural properties vary in response to a complex DWDS environment, which will contribute to further research on the risk assessment and control of biofilm-associated-pathogens in DWDS.

Role of biofilm roughness and hydrodynamic conditions in Legionella pneumophila adhesion to and detachment from simulated drinking water biofilms.

Biofilms in drinking water distribution systems (DWDS) could exacerbate the persistence and associated risks of pathogenic Legionella pneumophila (L. pneumophila), thus raising human health concerns. However, mechanisms controlling adhesion and subsequent detachment of L. pneumophila associated with biofilms remain unclear. We determined the connection between L. pneumophila adhesion and subsequent detachment with biofilm physical structure characterization using optical coherence tomography (OCT) imaging technique. Analysis of the OCT images of multispecies biofilms grown under low nutrient condition up to 34 weeks revealed the lack of biofilm deformation even when these biofilms were exposed to flow velocity of 0.7 m/s, typical flow for DWDS. L. pneumophila adhesion on these biofilm under low flow velocity (0.007 m/s) positively correlated with biofilm roughness due to enlarged biofilm surface area and local flow conditions created by roughness asperities. The preadhered L. pneumophila on selected rough and smooth biofilms were found to detach when these biofilms were subjected to higher flow velocity. At the flow velocity of 0.1 and 0.3 m/s, the ratio of detached cell from the smooth biofilm surface was from 1.3 to 1.4 times higher than that from the rough biofilm surface, presumably because of the low shear stress zones near roughness asperities. This study determined that physical structure and local hydrodynamics control L. pneumophila adhesion to and detachment from simulated drinking water biofilm, thus it is the first step toward reducing the risk of L. pneumophila exposure and subsequent infections.

Roles of ionic strength and biofilm roughness on adhesion kinetics of Escherichia coli onto groundwater biofilm grown on PVC surfaces.

Mechanisms of Escherichia coli attachment on biofilms grown on PVC coupons were investigated. Biofilms were grown in CDC reactors using groundwater as feed solution over a period up to 27 weeks. Biofilm physical structure was characterized at the micro- and meso-scales using Scanning Electron Microscopy (SEM) and Optical Coherence Tomography (OCT), respectively. Microbial community diversity was analyzed with Terminal Restricted Fragment Length Polymorphism (T-RFLP). Both physical structure and microbial community diversity of the biofilms were shown to be changing from 2 weeks to 14 weeks, and became relatively stable after 16 weeks. A parallel plate flow chamber coupled with an inverted fluorescent microscope was also used to monitor the attachment of fluorescent microspheres and E. coli on clean PVC surfaces and biofilms grown on PVC surfaces for different ages. Two mechanisms of E. coli attachment were identified. The adhesion rate coefficients (kd) of E. coli on nascent PVC surfaces and 2-week biofilms increased with ionic strength. However, after biofilms grew for 8 weeks, the adhesion was found to be independent of solution chemistry. Instead, a positive correlation between kd and biofilm roughness as determined by OCT was obtained, indicating that the physical structure of biofilms could play an important role in facilitating the adhesion of E. coli cells.

Role of divalent cations on deposition of Cryptosporidium parvum oocysts on natural organic matter surfaces.

A Radial Stagnation Point Flow (RSPF) system coupled with a microscope was used to study deposition of Cryptosporidium parvum oocysts on quartz and Suwannee River Natural Organic Matter (SRNOM)-coated surfaces in solutions with different Ca(2+) or Mg(2+) concentrations. Both untreated and proteinase K-treated oocysts were used. Deposition of oocysts on a SRNOM surface in Ca(2+) solution was higher than in Mg(2+) solution, even though the energy barriers calculated from Derjaguin-Landau-Verwey-Overbeek (DLVO) theory for Ca(2+) solution were higher than for Mg(2+) solution. On the other hand, the attachment of oocysts on a quartz surface was the same in both Ca(2+) and Mg(2+) solution and in qualitative agreement with the DLVO energy profiles. Inductive coupled plasma (ICP) was employed to measure the free divalent cation concentration in solutions containing oocysts. ICP data showed more Ca(2+) bound to oocyst surface than Mg(2+). Moreover, proteinase K treatment of oocysts led to a significant decrease in deposition rate due to less binding of Ca(2+) to the surface of the treated oocysts as shown by the ICP data. The deposition and ICP results suggested that inner-sphere complexation of Ca(2+) with carboxylate groups on both SRNOM and oocyst surfaces enhanced deposition of oocysts on a SRNOM surface.

Deposition of Cryptosporidium parvum oocysts on natural organic matter surfaces: microscopic evidence for secondary minimum deposition in a radial stagnation point flow cell.

A radial stagnation point flow (RSPF) system combined with a microscope was used to determine the deposition kinetics of Cryptosporidium parvum oocysts on quartz surfaces and silica surfaces coated with Suwannee River natural organic matter (SRNOM) in solutions with different ionic strengths. Microscopic evidence of C. parvum oocysts entrapped in the secondary minimum energy well was presented to show that among the entrapped C. parvum oocysts some were washed away by the radial flow and some were able to transfer to deep primary minima and become irreversibly deposited. Experimental data were compared with simulation results obtained by the convective-diffusion equation and Derjaguin-Landau-Verwey-Overbeek (DLVO) theory. The experimental results suggested that surface charge heterogeneity led to a higher attachment efficiency at low ionic strength. In addition, the maximum attachment efficiency was less than 1 at high ionic strength due to steric interaction.