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1.
J Biol Chem ; 275(15): 11026-33, 2000 Apr 14.
Article in English | MEDLINE | ID: mdl-10753905

ABSTRACT

The pH dependence of matrix metalloproteinase (MMP) catalysis is described by a broad bell-shaped curve, indicating the involvement of two unspecified ionizable groups in proteolysis. Stromelysin-1 has a third pK(a) near 6, resulting in a uniquely sharp acidic catalytic optimum, which has recently been attributed to His(224). This suggests the presence of a critical, but unidentified, S1' substructure. Integrating biochemical characterizations of inhibitor-enzyme interactions with active site topography from corresponding crystal structures, we isolated contributions to the pH dependence of catalysis and inhibition of active site residues Glu(202) and His(224). The acidic pK(a) 5.6 is attributed to the Glu(202).zinc.H(2)O complex, consistent with a role for the invariant active site Glu as a general base in MMP catalysis. The His(224)-dependent substructure is identified as a tripeptide (Pro(221)-Leu(222)-Tyr(223)) that forms the substrate cleft lower wall. Substrate binding induces a beta-conformation in this sequence, which extends and anchors the larger beta-sheet of the enzyme. substrate complex and appears to be essential for productive substrate binding. Because the PXY tripeptide is strictly conserved among MMPs, this "beta-anchor" may represent a common motif required for macromolecular substrate hydrolysis. The striking acidic profile of stromelysin-1 defined by the combined ionization of Glu(202) and His(224) allows the design of highly selective inhibitors.


Subject(s)
Matrix Metalloproteinase 3/metabolism , Binding Sites , Catalytic Domain , Humans , Hydrogen-Ion Concentration , Matrix Metalloproteinase 3/chemistry , Matrix Metalloproteinase Inhibitors , Protein Structure, Secondary
2.
J Biol Chem ; 274(35): 24881-7, 1999 Aug 27.
Article in English | MEDLINE | ID: mdl-10455161

ABSTRACT

For an animal model to predict a compound's potential for treating human disease, inhibitor interactions with the cognate enzymes of separate species must be comparable. Rabbit and human isoforms of stromelysin-1 are highly homologous, yet there are clear and significant compound-specific differences in inhibitor potencies between these two enzymes. Using crystal structures of discordant inhibitors complexed with the human enzyme, we generated a rabbit enzyme homology model that was used to identify two unmatched residues near the active site that could explain the observed disparities. To test these observations, we designed and synthesized three chimeric mutants of the human enzyme containing the single (H224N and L226F) and double (H224N/L226F) mutations. A comparison of inhibitor potencies among the mutant and wild-type enzymes shows that the mutation of a single amino acid in the human enzyme, histidine 224 to asparagine, is sufficient to change the selectivity profile of the mutant to that of the rabbit isoform. These studies emphasize the importance of considering species differences, which can result from even minor protein sequence variations, for the critical enzymes in an animal disease model. Homology modeling provides a tool to identify key differences in isoforms that can significantly affect native enzyme activity.


Subject(s)
Enzyme Inhibitors/pharmacology , Matrix Metalloproteinase Inhibitors , Amino Acid Sequence , Animals , Binding Sites , Computer Simulation , Enzyme Inhibitors/chemistry , Humans , Kinetics , Models, Molecular , Molecular Sequence Data , Molecular Structure , Mutagenesis, Site-Directed , Protein Isoforms/antagonists & inhibitors , Rabbits , Recombinant Fusion Proteins/genetics , Species Specificity
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