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Anal Biochem ; 146(1): 180-3, 1985 Apr.
Article in English | MEDLINE | ID: mdl-3887985

ABSTRACT

A simple assay of lysine decarboxylase is described. It is based on measuring the rate of titration of OH- ions, released during decarboxylation with the aid of a pH-stat apparatus. The continuous recording of the reaction progress may be easily performed. Using the pH-stat method the Km for lysine decarboxylase from E. coli is 2.0 mM.


Subject(s)
Carboxy-Lyases/analysis , Chemical Phenomena , Chemistry , Escherichia coli/enzymology , Hydrogen-Ion Concentration , Indicator Dilution Techniques
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