1.
Anal Biochem
; 146(1): 180-3, 1985 Apr.
Article
in English
| MEDLINE
| ID: mdl-3887985
ABSTRACT
A simple assay of lysine decarboxylase is described. It is based on measuring the rate of titration of OH- ions, released during decarboxylation with the aid of a pH-stat apparatus. The continuous recording of the reaction progress may be easily performed. Using the pH-stat method the Km for lysine decarboxylase from E. coli is 2.0 mM.