Facile Modification of Medical-Grade Silicone for Antimicrobial Effectiveness and Biocompatibility: A Potential Therapeutic Strategy against Bacterial Biofilms.

A one-step modification of biomedical silicone tubing with N,N-dimethyltetradecylamine, C14, results in a composition designated WinGard-1 (WG-1, 1.1 wt % C14). A surface-active silicon-amine phase (SAP) is proposed to account for increased wettability and increased surface charge. To understand the mechanism of antimicrobial effectiveness, several procedures were employed to detect whether C14 leaching occurred. An immersion-growth (IG) test was developed that required knowing the bacterial Minimum Inhibitory Concentrations (MICs) and Minimum Biocidal Concentrations (MBCs). The C14 MIC and MBC for Gm- uropathogenic E. coli (UPEC), commonly associated with catheter-associated urinary tract infections (CAUTI), were 10 and 20 µg/mL, respectively. After prior immersion of WG-1 silicone segments in a growth medium from 1 to 28 d, the IG test for the medium showed normal growth for UPEC over 24 h, indicating that the concentration of C14 must be less than the MIC, 10 µg/mL. GC-MS and studies of the medium inside and outside a dialysis bag containing WG-1 silicone segments supported de minimis leaching. Consequently, a 5 log UPEC reduction (99.999% kill) in 24 h using the shake flask test (ASTM E2149) cannot be due to leaching and is ascribed to contact kill. Interestingly, although the MBC was greater than 100 µg/mL for Pseudomonas aeruginosa, WG-1 silicone affected an 80% reduction via a 24 h shake flask test. For other bacteria and Candida albicans, greater than 99.9% shake flask kill may be understood by proposing increased wettability and concentration of charge illustrated in the TOC. De minimis leaching places WG-1 silicone at an advantage over conventional anti-infectives that rely on leaching of an antibiotic or heavy metals such as silver. The facile process for preparation of WG-1 silicone combined with biocidal effectiveness comprises progress toward the goals of device designation from the FDA for WG-1 and clearance.

Icephobic, Pt-Cured, Polydimethylsiloxane Nanocomposite Coatings.

To explore novel coatings with potential for easy release of ice (icephobicity), a series of platinum-cured silicone coatings was prepared incorporating SYL-OFF 7210, designated MQ-R, as a nanoscale reinforcing component. These optically transparent coatings are designated according to cure temperature and MQ-R wt %, for example, Pt-PDMS(25)-20 for 25 °C cure and 20 wt % MQ-R. Surface characterization included dynamic contact angles and morphology by atomic force microscopy. Bulk characterization was accomplished with stress-strain measurements at 25 °C and dynamic mechanical analysis from -110 to 150 °C. Ice adhesion tests at -10 °C showed modulus had a dominant effect in increasing τice, the peak removal force. At -30 °C, storage modulus was greater for coatings cured at 100 °C compared to 25 °C, but ice removal tests at -30 °C (-22 °F) consistently showed τice for Pt-PDMS(100) MQ-R compositions was less than τice for corresponding Pt-PDMS(25) coatings. This unexpected result was explained by proposing that supercooled water at hydrophilic interfacial sites (-10 °C) does not impede ice removal but frozen water pins ice at -30 °C. Interestingly, MQ-R was found to be a reactive filler that increased modulus after 100 °C cure especially for Pt-PDMS(100)-30 (3 MPa) and Pt-PDMS(100)-40 (5 MPa). In summary, by virtue of resistance to ice adhesion Pt(PDMS) coatings with low MQ-R content have potential for conferring energy savings and safety while high MQ-R content results in noteworthy mechanical properties.