ABSTRACT
As part of an effort to develop a model system for somatic gene therapy, a human argininosuccinate synthetase (AS) cDNA has been introduced into two gag+ retroviral vectors, and high titer clones were obtained for both constructs. The presence of proviral DNA sequences was detected in individual spleen colonies after infection of primary murine marrow cells with each virus. Mice were reconstituted for long-term survival with marrow infected with one virus, and they demonstrated elevated levels of AS expression in peripheral blood for up to eight weeks posttransplantation.
Subject(s)
Argininosuccinate Synthase/biosynthesis , Bone Marrow/microbiology , Cloning, Molecular , Ligases/biosynthesis , Animals , Argininosuccinate Synthase/blood , Argininosuccinate Synthase/genetics , Blotting, Southern , Bone Marrow Cells , Bone Marrow Transplantation , Cells, Cultured , Gene Products, gag , Genetic Vectors , Humans , Mice , Nucleotide Mapping , Retroviridae/genetics , Retroviridae Proteins/genetics , Ribonuclease, Pancreatic , Spleen/cytology , Spleen/microbiologyABSTRACT
A prior study (1970) of parasites in fecal samples of 846 dogs recovered from an area in the Edgewater and Rogers Park areas on the far North side of Chicago showed 13.7% of the fecal samples to be positive for one or more parasites. A pilot study of 200 fecal samples in the same area in 1979 revealed that 21.5% were positive. An additional 606 fecal samples were then observed for a total of 806 samples in 1979-1980. A prevalence of 23.4% was shown in the specimens collected in 1979. A study of 110 fecal samples from the Chicago dog pound revealed a prevalence of 71.8%.
Subject(s)
Animals, Domestic/parasitology , Dog Diseases/parasitology , Intestinal Diseases, Parasitic/epidemiology , Suburban Population , Animals , Chicago , Dog Diseases/epidemiology , Dogs , Feces/parasitology , Helminthiasis/epidemiology , Intestinal Diseases, Parasitic/veterinary , Socioeconomic FactorsABSTRACT
The transient colonization of the female genital tract with an unusual multiflagellated protozoan is reported. The presence of active organisms in large numbers in the mucus of the endocervical canal and their absence from the gastrointestinal tract and other areas indicates true colonization rather than contamination. The colonization resolved spontaneously. The organism has been classified as a Polymastigidan.