ABSTRACT
Bovine tropical theileriosis caused by Theileria annulata, is a serious constraint to Indian dairy industry with more fatal infections in exotic cattle and substantial losses to cross-bred and indigenous zebu cattle. The present communication is to place on record the first report of molecular based confirmed case of cerebral theileriosis caused by T. annulata coupled with its morphological detection, clinical manifestations, haematological alterations and therapeutic management in a cross bred cattle calf from India. After preparation of peripheral thin blood smear from cross bred cattle calf at the site of collection and fixation with methanol, blood sample brought to Department of Veterinary Parasitology, College of Veterinary Science and A.H, Jabalpur and stained by standard protocol for Giemsa staining. Genomic DNA was isolated from the collected blood sample using QIAamp® DNA blood mini kit following the manufacturer's recommendations and PCR was performed. The cross bred cow calf revealed high rise in temperature (105.5°F), increased heart rate, labored breathing with seromucous nasal discharge, enlargement of prescapular lymph node and animal exhibited tonic clonic convulsions in response to any sudden noise. Giemsa stained thin blood smear revealed intraerythrocytic piroplasm and Koch'sblue bodies of T. annulata within the cytoplasm of lymphocytes. The species of Theileria was confirmed by molecular amplification of genomic DNA as T. annulata.
ABSTRACT
Gastrointestinal (GI) parasitism in animals is considered as one of the major problems in India causing recurring economic losses to livestock production system owing to morbidity and mortality. But data pertaining to epidemiology of GI nematodes in goats is meager from Malwa region of Madhya Pradesh. Therefore, the present investigation was carried out to study the incidence of GI nematodes in goats in and around Mhow and accordingly a total of 900 faecal samples of goats were collected from Mhow and its adjoining areas and faecal sample examination revealed the incidence of strongyle, Strongyloides papillosus, Trichuris spp. and mixed infection from the study area. Significantly higher infection of GI nematodes was evidenced in the monsoon season (50.67%) when compared with summer (41.33%) and winter (38.33%) seasons. Significantly higher infection rate was documented in >1 year-old-goats (50.43%) than <1 year-old-goats (19.31%). Non-significantly higher infection was noted in females (46.04%) than their male counterparts (39.50%).
