ABSTRACT
AIMS: The aim of this study was to analyze the tobacco-related genotoxic effects in individual with habit of smoking and chewing tobacco. MATERIALS AND METHODS: The present study sample consisted of 120 individuals attending the outpatient department of D. J. College of Dental Sciences and Research, Modinagar, Uttar Pradesh (UP). The sample was divided into four groups as follows: Group I (individuals with habit of smoking tobacco), Group II (individuals with habit of chewing tobacco), Group III (individuals with habit of smoking and chewing tobacco), and Group IV control group (nontobacco-exposed individuals). Patients were asked to rinse their mouth gently with water. The exfoliated cells were obtained by scraping the buccal mucosa of individuals with a wooden spatula. The scraped cells were placed on the precleaned slides. The smears were then stained with RAPID-PAP™ and analyzed under the microscope. Data were analyzed using SPSS statistical software. RESULTS: In the present study, an arbitrary unit was obtained using frequency/day multiplied by the duration of years (risk multiplication factor [RMF], a positive and significant correlation were observed between the RMF and the mean percentage of micronucleated cell count in smokers, chewers, and in individuals with both smoking and chewing habit, respectively. A weak positive and nonsignificant correlation were observed between age and mean percentage of micronucleated cells in smokers and smokers + chewers, respectively, while it was weak negative and nonsignificant in chewers. In control group, correlation between age and percentage of micronucleated cells was weak positive and nonsignificant at 5% level of significance. CONCLUSION: The micronuclei in exfoliated mucosal cells from buccal mucosa can be used as a biomarker of genotoxicity in predicting the effects of carcinogens.
ABSTRACT
Maxillary lateral incisors are most common teeth to be found missing. They also are the most common teeth that need esthetic replacement. A 23-year-old female patient with missing maxillary lateral incisors was treated orthodontically: laterals were replaced with implants. Challenges while doing this case are discussed in the following case report.
ABSTRACT
Aims: The aim of this study was to analyze the tobacco-related genotoxic effects in individual with habit of smoking and chewing tobacco. Materials and Methods: The present study sample consisted of 120 individuals attending the outpatient department of D. J. College of Dental Sciences and Research, Modinagar, Uttar Pradesh (UP). The sample was divided into four groups as follows: Group I (individuals with habit of smoking tobacco), Group II (individuals with habit of chewing tobacco), Group III (individuals with habit of smoking and chewing tobacco), and Group IV control group (nontobacco-exposed individuals). Patients were asked to rinse their mouth gently with water. The exfoliated cells were obtained by scraping the buccal mucosa of individuals with a wooden spatula. The scraped cells were placed on the precleaned slides. The smears were then stained with RAPID-PAP⢠and analyzed under the microscope. Data were analyzed using SPSS statistical software. Results: In the present study, an arbitrary unit was obtained using frequency/day multiplied by the duration of years (risk multiplication factor [RMF], a positive and significant correlation were observed between the RMF and the mean percentage of micronucleated cell count in smokers, chewers, and in individuals with both smoking and chewing habit, respectively. A weak positive and nonsignificant correlation were observed between age and mean percentage of micronucleated cells in smokers and smokers + chewers, respectively, while it was weak negative and nonsignificant in chewers. In control group, correlation between age and percentage of micronucleated cells was weak positive and nonsignificant at 5% level of significance. Conclusion: The micronuclei in exfoliated mucosal cells from buccal mucosa can be used as a biomarker of genotoxicity in predicting the effects of carcinogens
Subject(s)
India , Mouth Neoplasms , RiskABSTRACT
AIM: The high incidence of nickel (Ni) allergy and the increasing use of Ni-containing dental biomaterials have been of growing concern. The purpose of this investigation was to analyze and evaluate the rate of Ni ion release from different types arch wires used in orthodontics. MATERIALS AND METHODS: Four groups of arch wires (nickel titanium [NiTi], SS, Cu NiTi and ion implanted NiTi) with twelve samples were stored in artificial saliva with a pH 5.6-7.0 thermostated at (36.5°C) and tested at different intervals i.e., 7(th) day, 14(th) day, and 21(st) day. The amount of Ni and Ti ions released from the sample were evaluated using an atomic adsorption spectrophotometer. The solution was replaced with a fresh bottle to avoid sediments. RESULTS: Statistical analysis was performed by nonparametric tests (Student's paired t-test, one-way analysis of variance and multiple comparison test by Tukey "Honestly significant difference"). The statistical package SPSS PC plus (version 4.0.1) was used for data processing and statistical analysis. Results showed significantly statistical influence on the release amount of Ni and Ti ions. Large variation in concentration of Ni released from brackets and bands combined. However, the amount of Ni ions released in all test solutions diminished with time and was below the critical value necessary to induce allergy and below daily dietary intake level. CONCLUSIONS: The daily release of NiTi, SS, Cu NiTi and ion implanted NiTi by an orthodontic appliance in acid pH, particularly favorable to corrosion, was well below that ingested with a normal daily diet. It is therefore concluded that the quantities of metal ions released in our experimental conditions should not be cause for concern in utilizing the appliance.
