ABSTRACT
A column flow-through radioimmune assay for serum myoglobin has been developed. The assay uses antibodies to human or rhesus monkey myoglobin coupled to Sepharose by cyanogen bromide activation and myoglobin labeled by the Chloramine-T method. The labeled myoglobin is stable over two to three half-lives. Serum myoglobin levels were elevated when serum was taken up to 12 hours after myocardial infarction and generally elevated up to 24 hours. A continued or repeated elevation of serum myoglobin probably indicates continued myocardial damage or new attacks. In the cases of myocardial infarction, levels up to 1340 ng per ml were found.
Subject(s)
Myoglobin/blood , Animals , Antibody Specificity , Binding Sites, Antibody , Chromatography , Haplorhini , Humans , Myocardial Infarction/blood , Myoglobin/immunology , Myoglobinuria , Radioimmunoassay , Species SpecificityABSTRACT
Seven synthetic analogues of somatostatin helped clarify structural requirements for suppression of growth hormone secretion in rats. Size of the ring is not critical; deletions of serine-13, lysine-4 or asparagine-5 result in peptides which retain an appreciable fraction of the activity. The analogue des-Ala1, Gly2, Asn5-somatostatin lowers plasma growth hormone and insulin levels without affecting plasma glucagon levels significantly.
Subject(s)
Somatostatin/analogs & derivatives , Amino Acid Sequence , Animals , Glucagon/blood , Growth Hormone/blood , Insulin/blood , Methods , Rats , Somatostatin/chemical synthesis , Somatostatin/pharmacology , Structure-Activity RelationshipABSTRACT
PIP: The effects of oral contraceptives on serum lipids were investigated in humans and monkeys at the Temple University Health Sciences Center. The compositions of the pills were: 1) 2.0 mg chlormadinone acetate, .08 mg mestranol; 2) 10.0 mg medroxyprogesterone acetate, .05 mg ethinyl estradiol; 3) 1.0 mg ethynodiol diacetate, .1 mg mestranol; 4) 1.0 mg norethindrone acetate, .05 mg ethinyl estradiol, and 5) .5 mg norgestrel, .05 mg ethinyl estradiol. 238 immediately puerperal black women, healthy and aged 20-30, were divided into 6 groups for study purposes; each of 5 groups used a separate oral therapy, while the sixth group (control) was fitted with a plastic intrauterine device. 75 women were still participating in the study at 54 weeks. All of the drugs significantly increased the concentrations of serum cholesterol, serum triglycerides, total phospholipids, and fatty acids. The influence of the drugs on lipoproteins was variable. The higher its progestogen/estrogen ratio, the greater influence a drug had on lipid concentrations. Some of the same effects were seen in 14 mature female squirrel monkeys (Cebus albifrons), which were studied in a similar manner for about 30 cycles; however, only drugs 1, 2, and 3 were used in this part of the study. Clinical observations of the patients in this study, showed no relationship between serum phosphatides participating in the coagulation mechanism and the production of thromboembolic phenomena. The researchers found no justification for the delay of oral contraceptive prescription after childbirth.^ieng
