ABSTRACT
Allele frequencies and forensic parameters for 15 autosomal loci included in the AmpFlSTR® Identifiler kit were estimated in a sample of 57 unrelated Afro-descendants from Nor Yungas (Bolivia). Buccal swabs samples were obtained from voluntary donors, after consent was given. All loci were in Hardy-Weinberg equilibrium after Bonferroni correction. D21S11 was the most informative locus, while the least discriminating locus was D3S1358. The combined power of discrimination and the combined probability of exclusion were >0.99999999 and >0.99997, respectively. The multidimensional scaling (MDS) plot generated by Rst matrix supported that Afro-Bolivians of Nor Yungas preserved a stronger African descent compared to other admixed Latin American populations. These results amplified the Bolivian databases of autosomal STR loci and may provide a useful tool for human identification tests and population genetic studies.
Subject(s)
Black People/genetics , Chromosomes, Human/genetics , DNA Fingerprinting/methods , Ethnicity/genetics , Gene Frequency/genetics , Genetics, Population , Microsatellite Repeats/genetics , Bolivia , Female , Forensic Genetics , Genetic Carrier Screening , Genetic Loci , Humans , Male , PedigreeABSTRACT
Allele frequencies for the 15 short tandem repeats (STRs) loci included in the AmpFlSTR® Identifiler kit were estimated in a sample of unrelated individuals from Mar del Plata (MDQ; N = 180) and Bahia Blanca (BB; N = 85) (Buenos Aires, Argentina). Biological samples were obtained from voluntary donors and forensic cases. Both populations were in Hardy-Weinberg equilibrium after Bonferroni correction, except for locus vWA in MDQ and D2S1338 in BB. FGA was the most informative locus, and the least discriminating locus was TPOX in both samples. The combined power of discrimination (PDc) and the combined probability of exclusion (PEc) were similar in MDQ and BB samples (0.999999998 < PDc < 0.999999999 and 0.999999979 < PEc < 0.999999989). The multidimentional scaling plot from Rst genetic distance matrix and the interethnic admixture estimation supported a higher European contribution in populations of the central region compared with populations from other regions of Argentina with higher Amerindian composition. These results enlarge the Argentine databases of autosomal STR loci, revealed as an excellent tool for human identification tests and population genetic analysis.
