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1.
Cell Microbiol ; 22(1): e13129, 2020 01.
Article in English | MEDLINE | ID: mdl-31652367

ABSTRACT

Phagocytic cells ingest bacteria by phagocytosis and kill them efficiently inside phagolysosomes. The molecular mechanisms involved in intracellular killing and their regulation are complex and still incompletely understood. Dictyostelium discoideum has been used as a model to discover and to study new gene products involved in intracellular killing of ingested bacteria. In this study, we performed random mutagenesis of Dictyostelium cells and isolated a mutant defective for growth on bacteria. This mutant is characterized by the genetic inactivation of the lrrkA gene, which encodes a protein with a kinase domain and leucine-rich repeats. LrrkA knockout (KO) cells kill ingested Klebsiella pneumoniae bacteria inefficiently. This defect is not additive to the killing defect observed in kil2 KO cells, suggesting that the function of Kil2 is partially controlled by LrrkA. Indeed, lrrkA KO cells exhibit a phenotype similar to that of kil2 KO cells: Intraphagosomal proteolysis is inefficient, and both intraphagosomal killing and proteolysis are restored upon exogenous supplementation with magnesium ions. Bacterially secreted folate stimulates intracellular killing in Dictyostelium cells, but this stimulation is lost in cells with genetic inactivation of kil2, lrrkA, or far1. Together, these results indicate that the stimulation of intracellular killing by folate involves Far1 (the cell surface receptor for folate), LrrkA, and Kil2. This study is the first identification of a signalling pathway regulating intraphagosomal bacterial killing in Dictyostelium cells.


Subject(s)
Dictyostelium/enzymology , Folic Acid/metabolism , Phagosomes/microbiology , Phosphotransferases/metabolism , Protozoan Proteins/metabolism , Signal Transduction , Dictyostelium/genetics , Dictyostelium/microbiology , Gene Expression Regulation, Bacterial , Intracellular Space/microbiology , Klebsiella pneumoniae/metabolism , Leucine/chemistry , Phagocytosis , Phosphotransferases/genetics , Protein Domains , Protozoan Proteins/genetics
2.
Proc Natl Acad Sci U S A ; 114(31): 8277-8282, 2017 08 01.
Article in English | MEDLINE | ID: mdl-28716905

ABSTRACT

MitoNEET (mNEET) is a dimeric mitochondrial outer membrane protein implicated in many facets of human pathophysiology, notably diabetes and cancer, but its molecular function remains poorly characterized. In this study, we generated and analyzed mNEET KO cells and found that in these cells the mitochondrial network was disturbed. Analysis of 3D-EM reconstructions and of thin sections revealed that genetic inactivation of mNEET did not affect the size of mitochondria but that the frequency of intermitochondrial junctions was reduced. Loss of mNEET decreased cellular respiration, because of a reduction in the total cellular mitochondrial volume, suggesting that intermitochondrial contacts stabilize individual mitochondria. Reexpression of mNEET in mNEET KO cells restored the WT morphology of the mitochondrial network, and reexpression of a mutant mNEET resistant to oxidative stress increased in addition the resistance of the mitochondrial network to H2O2-induced fragmentation. Finally, overexpression of mNEET increased strongly intermitochondrial contacts and resulted in the clustering of mitochondria. Our results suggest that mNEET plays a specific role in the formation of intermitochondrial junctions and thus participates in the adaptation of cells to physiological changes and to the control of mitochondrial homeostasis.


Subject(s)
Cell Respiration/genetics , Iron-Binding Proteins/genetics , Iron-Binding Proteins/metabolism , Membrane Proteins/genetics , Membrane Proteins/metabolism , Mitochondria/metabolism , Animals , CRISPR-Cas Systems , Cells, Cultured , Gene Knockout Techniques , Hydrogen Peroxide/pharmacology , Mice , Mitochondria/genetics , Mitochondria/pathology , Oxidative Stress/genetics
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