ABSTRACT
Matrix metalloproteinase-9 (GELB) as a member of gelatinases plays key role in the destruction of blood-brain barrier (BBB), T cells migration into the CNS, and demyelination induction. Considering remyelination induction in response to tart cherry extract and pure p-coumaric acid ingestion via tracking MMP9 gene expression in the cuprizone mouse model. Firstly, predicting the chemical interaction between p-coumaric acid and MMP9 protein was conducted through PASS and Swiss dock web services. Next, the content of p-coumaric acid in the tart cherry extract was analyzed by HPLC. Later, mice (male, female) were categorized into two groups: standard, cuprizone. After the demyelination period, mice classified into four groups: standard, natural chow, tart cherry extract, p-coumaric acid. Finally, brains were extracted from the skull, and MMP9 gene expression was evaluated by real time RT-PCR. Bioinformatics analysis displayed p-coumaric acid has potent inhibitory effect on MMP9 gene expression (Pa=0.818) with estimated ΔG (kcal/mol) -8.10. In addition, during the demyelination period, MMP9 expression was increased significantly in the male group that is related to myelin destruction. However, MMP9 was declined throughout remyelination in both male and female. It's remarkable that pure p-coumaric acid and tart cherry extract ingestion could decrease the gene expression ratio more than natural chow. According to the results, it's deduced the male mouse is more appropriate gender for demyelination induction via cuprizone. In addition, tart cherry extract and pure p-coumaric acid ingestion could decrease MMP9 gene expression level considerably during remyelination.
Subject(s)
Matrix Metalloproteinase 9/genetics , Myelin Sheath/metabolism , Propionates/pharmacology , Animals , Brain/metabolism , Coumaric Acids , Cuprizone/administration & dosage , Demyelinating Diseases , Disease Models, Animal , Female , Male , Mice , Mice, Inbred C57BL , RemyelinationABSTRACT
Leishmania spp. are vector-borne flagellates transferred by sand flies. They cause cutaneous, mucocutaneous and visceral infections in mammals, especially in humans and dogs. A mature male boxer with ulcerative nodules around his eyes and snout was referred to Small Animal Hospital, Faculty of Veterinary Medicine, University of Tehran. Multiple cutaneous lesions were seen in physical examination. Mild leukocytosis, neutrophilia, left shift, lymphopenia, and thrombocytopenia were reported by the laboratory. Diagnosis was confirmed by the observation of amastigotes in blood samples and inside tissue macrophages. The infection was treated using pentavalent antimonial drug for four weeks.