ABSTRACT
A field-portable anion exchange resin method (often cited as the Ficklin method (1983)) has been extensively used to distinguish between dissolved arsenite (As(III)) and arsenate (As(V)) species in natural waters. As(III) occurs largely as As(OH)(3), which is uncharged at ca. pH 7, while As(V) is negatively charged and will sorb to the resin. However, we show that negatively charged As(III)-sulfide (thioarsenite) species, important at sulfide concentrations >10 microM, also bind to the anion exchange resins, and therefore might be interpreted incorrectly as As(V). Furthermore, we show that nitrogen-purging, which results in a conversion of As(III)-sulfides to arsenite, can be used to obtain accurate arsenic speciation when resins are used on sulfidic water samples.
Subject(s)
Anion Exchange Resins/chemistry , Arsenic/chemistry , Arsenic/isolation & purification , Water Purification/methods , Adsorption , Hydrogen-Ion Concentration , Sulfides/chemistry , Water/chemistryABSTRACT
The extracellular speciation of mercury may control bacterial uptake and methylation. Mercury-polysulfide complexes have recently been shown to be prevalent in sulfidic waters containing zero-valent sulfur. Despite substantial increases in total dissolved mercury concentration, methylation rates in cultures of Desulfovibrio desulfuricans ND132 equilibrated with cinnabar did not increase in the presence of polysulfides, as expected due to the large size and charged nature of most of the complexes. In natural waters not at saturation with cinnabar, mercury-polysulfide complexes would be expected to shift the speciation of mercury from HgS(0)((aq)) toward charged complexes, thereby decreasing methylation rates.
